FROM POLLINATION TO DH LINES – VERIFICATION AND OPTIMIZATION OF PROTOCOL FOR PRODUCTION OF DOUBLED HAPLOIDS IN CUCUMBER
Joanna Gałązka
Warsaw University of Life Sciences – SGGWRenata Słomnicka
Warsaw University of Life Sciences – SGGWKatarzyna Góral-Radziszewska
Warsaw University of Life Sciences – SGGWKatarzyna Niemirowicz-Szczytt
Warsaw University of Life Sciences – SGGWAbstract
A complete set of procedures comprising of the induction of haploid embryo development by pollination with irradiated pollen, embryo isolation and in vitro culture, haploid plant development, chromosome doubling by regeneration from leaf explants, acclimatization, self-pollination of DH plants and assessment of DH lines was performed in cucumber. Haploid and doubled haploid plants were obtained from all eight genotypes used in the study. The donor plant genotypes were evaluated by comparing the number of haploid embryos and DH plants obtained from each genotype. The influence of the donor plant genotype and its age on the regeneration and the yield of DH plants were evaluated. In order to optimize the method of chromosome doubling, the composition of medium was modified and the explants were divided into four groups with regard to the leaf sector used for regeneration. Acclimatization of DH plants to the greenhouse conditions was improved. Uniform, useful in breeding, DH lines were obtained.
Keywords:
acclimatization, Cucumis sativus L., haploid induction, pollen irradiation, embryo rescue, flow cytometryReferences
Çağlar, G., Abak, K. (1996a). The effects of season and irradiation doses on haploid embryo production in cucumber (C. sativus L.). Proc. of the VIth Eucarpia meeting on Cucurbit genetics
and breeding, Malaga, Spain, 25 – 30.
Çağlar, G., Abak, K. (1996b). Efficiency of haploid production in cucumber. Cuc. Gen. Coop. Rep., 19, 36–37.
Diao, W.-P., Jia, Y.-Y., Song, H., Zhang, X.-Q., Lou, Q.-F., Chen, J.-F. (2009). Efficient embryo induction in cucumber ovary culture and homozygous identification of the regenerants using
SSR markers. Sci. Hortic. Amsterdam, 119(3), 246 – 251.
Dirks, R. (1996). Method for the production of double-haploid cucumbers. United States Patent No. 5, 492, 827.
Faris, N.M. (1999). Selected aspects of biotechnology in cucumber (Cucumis sativus L.) haploids. PhD thesis, Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture and Landscape Architecture, WULS-SGGW.
Faris, N.M., Rakoczy-Trojanowska, M., Malepszy, S., Niemirowicz-Szczytt, K. (2000). Diploidization of cucumber (Cucumis sativus L.) haploids by in vitro culture of leaf explant. In: Food
Biotechnology, Bielecki, S., Tramper, J., Polak, J. (eds). Elsevier Science B.V, 49–54.
Fila, G., Ghashghaie, J., Hoarau, J., Cornic, G. (1998). Photosynthesis, leaf conductance and water relations of in vitro cultured grapevine rootstock in relation to acclimatization. Physiol.
Plant., 102(3), 411– 418.
Gałązka, J., Niemirowicz-Szczytt, K. (2013). Review of research on haploid production in cucumber and other cucurbits. Folia Horticult., 25, 1, 67–78.
Gémes Juhász, A., Balogh, P., Ferenczy, A., Kristóf, Z. (2002). Effect of optimal stage of female gametophyte and heat treatment on in vitro gynogenesis induction in cucumber (Cucumis sativus L.). Plant Cell Rep., 21, 105–111.
Kwack, S.N., Fujieda, K. (1988). Somatic embryogenesis in cultured unfertilized ovules of Cucurbita moschata. J. Jpn. Soc. Hortic. Sci., 57, 34–42.
Lim, W., Earle, E.D. (2009). Enhanced recovery of doubled haploid lines from parthenogenetic plants of melon (Cucumis melo L.). Plant Cell Tiss. Org. Cult., 98, 351–356.
Machakova, I., Zazimalova, E., George, E.F. (2008). Plant growth regulators I: Introduction; Auxins, their analogues and inhibitors. In: Plant propagation by tissue culture, 3rd ed., George,
E.F., Hall, M.A., De Klerk, D.-J. (eds). Springer, 175–204.
Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15, 473–497.
Niemirowicz-Szczytt, K., Faris, N.M., Rucińska, M., Nikolova, V. (2000). Conservation and storage of haploid cucumber (Cucumis sativus L.) collection under in vitro conditions. Plant
Cell Rep., 19, 311–314.
Nikolova, V., Niemirowicz-Szczytt, K. (1996). Diploidization of cucumber (Cucumis sativus L.) haploids by colchicine treatment. Acta Soc. Bot. Pol., 65, 311–317.
Przyborowski, J.A., Niemirowicz-Szczytt, K. (1994). Main factors affecting cucumber (Cucumis sativus L.) haploid embryo development and haploid plant characteristics. Plant Breed., 112, 70–75.
Sauton, A., Dumas De Vaulx, R. (1987). Production of haploid plants in melon (Cucumis melo L.) as a result of gynogenesis induced by irradiated pollen. Agronomie, 7, 141–147.
Sztangret-Wiśniewska, J., Gałecka, T., Korzeniewska, A., Marzec, L., Kołakowska, G., Piskurewicz, U., Śmiech, M., Niemirowicz-Szczytt, K. (2006). Characteristics of double-haploid cucumber
(Cucumis sativus L.) lines resistant to downy mildew (Pseudoperonospora cubensis [Berk. et Curt.] Rostovzev). Proc. Cucurbitaceae, G.J. Holmes (ed.). Raleigh, North Carolina, USA, 515–526.
Usman, M., Hussain, Z., Fatima, B. (2011). Somatic embryogenesis and shoot regeneration induced in cucumber leaves. Pakistan J. Bot., 43 (2), 1283–1293.
Yesitir, H., Sari, N. (2003). A new method for haploid muskmelon (Cucumis melo L.) dihaploidization. Sci. Hort., 98, 277–283.
Warsaw University of Life Sciences – SGGW
Warsaw University of Life Sciences – SGGW
Warsaw University of Life Sciences – SGGW
Warsaw University of Life Sciences – SGGW
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