In silico comparative transcriptome analysis of Papaver somniferum cultivars
Tuğba Gürkök Tan
Cankiri Karatekin University, Food and Agriculture Vocational School, Department of Field Crops 18100, Cankiri, Turkeyhttps://orcid.org/0000-0003-0599-5628
Mine Türktaş
Gazi University, Department of Biology, 06100 Ankara, Turkeyhttps://orcid.org/0000-0001-8089-3774
Gülşen Güçlü
Sivas Cumhuriyet University, Health Services Vocational School, Department of Health Programmes, Sivas, Turkeyhttps://orcid.org/0000-0002-3599-213X
Abstract
Papaver somniferum is a medicinal plant of the Papaveraceae family that has traditionally been used for diet or its therapeutic value for thousands of years. Mainly, morphine and noscapine alkaloids exhibit anti-analgesic and anti-cancer effects. However, gene expression patterns and regulatory elements, such as transcription factors between different tissues, still need to be detected. In this study, comparative in silico transcriptome analyses were conducted to examine the tissue-specificity of the benzylisoquinoline alkaloids (BIAs) biosynthetic genes and transcription factors (TFs) between morphine and noscapine cultivars. Analysis showed that BIA biosynthetic genes are expressed in a different pattern between two varieties. Results showed that some members of plant-specific secondary metabolites related to TF families, such as MYB, MADS-box, bHLH, NAC, and WRKY, are differentially expressed between tissues and varieties.
Keywords:
BIA pathway genes, Papaver somniferum L., tissue specificity, transcription factors, transcriptomeReferences
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Cankiri Karatekin University, Food and Agriculture Vocational School, Department of Field Crops 18100, Cankiri, Turkey https://orcid.org/0000-0003-0599-5628
Gazi University, Department of Biology, 06100 Ankara, Turkey https://orcid.org/0000-0001-8089-3774
Sivas Cumhuriyet University, Health Services Vocational School, Department of Health Programmes, Sivas, Turkey https://orcid.org/0000-0002-3599-213X
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