The influence of exogenous gibberellic acid (GA3) and 24-epibrassinolide (24-EpiBL) on seed germination and the expression of genes involved in GA and BR synthesis/signalling in pepper (Capsicum annuum L.)
Selin Çayan
Ankara University, Faculty of Agriculture, Department of Horticulture, Ankara, TurkeyGölge Sarıkamış
Ankara University Faculty of Agriculture Department of HorticultureCanan Yüksel Özmen
Ankara University, Biotechnology Institute, Ankara, TurkeyUmut Kibar
Republic of Turkey, Ministry of Agriculture and Forestry, Agriculture and Rural Development Support Institution, Ankara, TurkeyEren Özden
Iğdır University, Faculty of Agriculture, Department of Horticulture, Iğdır, TurkeyAli Ergül
Ankara University, Biotechnology Institute, Ankara, TurkeyAbstract
Gibberellins (GAs) and brassinosteroids (BRs) are the plant hormones involved in various physiological processes including seed germination. In this study, the effects of exogenous gibberellic acid (GA3) and 24-epibrassinolide (24-EpiBL) treatments on the expression of key genes involved in GA and BR syntheis/signalling during seed germination were investigated in pepper (Capsicum annuum L).
The expressions of BES1 and BRI1 involved in BR synthesis/signalling pathway as well as GA3OX1 and GA20OX1 associated with gibberellic acid biosynthesis in plants were determined. Exogenous GA3 treatments increased BES1 expression and the highest increase was determined with 10⁻⁸ M BR + 100 µM GA3 (P<0.05). On the contrary, the expression of BRI1 gene was significantly decreased by 10-8 M BR + 100 µM GA3 (P<0.05). The expression of GA3OX1 gene was induced with BR and GA3 treatments (P<0.05). GA20OX1 gene expression was generally higher compared to the expression of GA3OX1 and significantly increased by the GA3 treatments. Our findings are expected to bring an insight to the influence of BRs during seed germination together with the expression of associated genes.
Keywords:
brassinosteroids, Capsicum annuum, expression, germination, gibberellins, pepperReferences
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Ankara University, Faculty of Agriculture, Department of Horticulture, Ankara, Turkey
Ankara University Faculty of Agriculture Department of Horticulture
Ankara University, Biotechnology Institute, Ankara, Turkey
Republic of Turkey, Ministry of Agriculture and Forestry, Agriculture and Rural Development Support Institution, Ankara, Turkey
Iğdır University, Faculty of Agriculture, Department of Horticulture, Iğdır, Turkey
Ankara University, Biotechnology Institute, Ankara, Turkey
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