TESTING OF SELF-(IN)COMPATIBILITY IN APRICOT CULTIVARS USING FLUORESCENCE MICROSCOPY
Dragan Milatović
University of Belgrade, SerbiaDragan Nikolić
University of Belgrade, SerbiaMilica Fotirić-Akšić
University of Belgrade, SerbiaAleksandar Radović
University of Belgrade, SerbiaAbstrakt
Self-incompatibility is common in apricot (Prunus armeniaca L.) cultivars of Central Asian and Irano-Caucasian ecogeographical groups, while cultivars of European
group are traditionally considered as self-compatible. However, the number of known self-incompatible cultivars of the European group has increased rapidly over the last two decades. This can be explained by using Asian or North American self-incompatible cultivars in breeding programs that aim to create new genotypes with the traits including: Plum Pox Virus resistance, frost tolerance, increase of the sugar content or extending the harvest time. In this work self-(in)compatibility was tested in 38 apricot cultivars. Pollentube growth in pistils pollinated in laboratory was analysed using fluorescence microscopy. Cultivars were considered self-compatible if at least one pollen tube reached the ovary in the majority of pistils. In self-incompatible cultivars growth of pollen tubes in the style of pistil stopped along with formation of characteristic swellings. Of the examined cultivars, 17 were self-compatible, and 21 were self-incompatible.
Słowa kluczowe:
Prunus armeniaca, pollination, pollen tube growth, pistilBibliografia
Alburquerque N., Egea J., Pérez-Tornero O., Burgos L., 2002. Genotyping apricot cultivars for self-(in)compatibility by means of RNases associated with S alleles. Plant Breed. 121, 343–347.
Andrés M.V., Durán J.M., 1998. Self-incompatibility in Spanish clones of apricot (Prunus armeniaca L.) tree. Euphytica 101, 349–355.
Audergon J.M., Guerriero R., Monteleone P., Viti R., 1999. Contribution to the study of inheritance of the character self-incompatibility in apricot. Acta Hortic. 488, 275–280.
Badenes M.L., Llácer G., 2006. Breeding for resistance: breeding for Plum pox virus resistant apricots (Prunus armeniaca L.) in Spain. EPPO Bulletin 36, 323–326.
Badenes M.L., Hurtado M.A., Sanz F., Archelos D.M., Burgos L., Egea J., Llácer G., 2000. Searching for molecular markers linked to male sterility and self-compatibility in apricot. Plant Breed. 119, 157–160.
Benediková D., 2006. Apricot breeding in Slovak Republic for fruit quality and resistance to disease – New Slovak apricot cultivars created by RBS Veselé. Acta Hortic. 701, 377–380.
Burgos L., Egea J., Guerriero R., Viti R., Monteleone P., Audergon J.M., 1997. The self-compatibility trait of the main apricot cultivars and new selections from breeding programmes. J. Hortic. Sci. 72, 147–154.
Burgos L., Pérez-Tornero O., Ballester J., Olmos E., 1998. Detection and inheritance of stylar ribonucleases associated with incompatibility alleles in apricot. Sex. Plant Reprod. 11, 153–158.
De Nettancourt D., 1977. Incompatibility in angiosperms. Springer-Verlag, Berlin, Heidelberg, New York.
Drén G., Szabó Z., Thurzó S., Racskó J., Soltész M., Nyéki J., 2007. Experiences on the fertilization of foreign apricot cultivars. Intern. J. Hortic. Sci. 13(3), 143–145.
Egea J., Burgos L., 1996. Detecting cross-incompatibility of three North American apricot cultivars and establishing the first incompatibility group in apricot. J. Am. Soc. Hortic. Sci. 121, 1002–1005.
Egea J., Garcia J.E., Egea L., Berenguer T., 1991. Self-incompatibility in apricot cultivars. Acta Hortic. 293, 285–293.
Entani T., Iwano M., Shiba H., Che F.S., Isogai A., Takayama S., 2003. Comparative analysis of the self-incompatibility (S-) locus region of Prunus mume: identification of a pollen-expressed F-box gene with allelic diversity. Genes Cells 8, 203–213.
Gharesheikhbayat R., Dondini L., Sansavini S., 2011. Identification of self-incompatibility allels in apricot (Prunus armeniaca L.) using multi-level approaches. Seed Plant Impr. J. 27, 411–426.
Guerriero R., Bartolini S., 1995., Flower biology in apricot: main aspects and problems. Acta Hortic. 384, 261–271.
Hajilou J., Grigorian V., Mohammadi S.A., Nazemmieh A., Romero C., Vilanova S., Burgos L., 2006. Self- and cross-(in)compatibility between important apricot cultivars in northwest Iran. J. Hortic. Sci. Biotech. 81, 513–517.
Halász J., Hegedűs A., Hermán R., Stefanovits-Bányai É., Pedryc A., 2005. New selfincompatibility alleles in apricot (Prunus armeniaca L.) revealed by stylar ribonuclease assay and S-PCR analysis. Euphytica 145, 57–66.
Halász J., Pedryc A., Hegedűs A., 2007. Origin and dissemination of the pollen-part mutated SChaplotype that confers self-compatibility in apricot (Prunus armeniaca). New Phytol. 176, 792–803.
Halász J., Pedryc A., Ercisli S., Yilmaz K.U., Hegedűs A., 2010. S-genotyping supports the genetic relationships between Turkish and Hungarian apricot germplasm. J. Am. Soc. Hortic. Sci. 135, 410–417.
Jie Q., Shupeng G., Jixiang Z., Manru G., Huairui S., 2005. Identification of self-incompatibility genotypes of apricot (Prunus armeniaca L.) by S-allele-specific PCR analysis. Biotechnol. Lett. 27, 1205–1209.
Karayiannis I., 2006. Breeding for resistance: conventional breeding for Plum pox virus resistant apricots (Prunus armeniaca L.) in Greece. EPPO Bull. 36, 319–322.
Karayiannis I., Thomidis T., Tsaftaris A., 2008. Inheritance of resistance to Plum pox virus in apricot (Prunus armeniaca L.). Tree Genet. Genom. 4, 143–148.
Kostina K.F., 1970. Issledovanija po samoopyleniju abrikosa. Trudy Gosud. Nikit. Bot. Sada 45, 7–17.
Krška B., Salava J., Polák J., 2006a. Breeding for resistance: breeding for Plum pox virus resistant apricots (Prunus armeniaca L.) in the Czech Republic. EPPO Bull. 36, 330–331.
Krška B., Vachůn Z., Nečas T., 2006b. The apricot breeding programme at the Horticulture Faculty in Lednice. Acta Hortic. 717, 145–148.
Layne R.E.C., Bailey C.H., Hough L.F., 1996. Apricots. In: Fruit breeding. Vol. I: Tree and tropical fruits, Janick J., Moore, J.N. (eds.). John Wiley, Inc., New York, 79–111.
Ledbetter C.A., Ramming D.W., 1997. Apricot Cv. Robada. USA Plant Patent No. 9, 890.
Ledbetter C., Peterson S., Jenner J., 2006. Modification of sugar profiles in California adapted apricots (Prunus armeniaca L.) through breeding with Central Asian germplasm. Euphytica 148, 251–259.
Martin F.W., 1959. Staining and observing pollen tubes in the style by means of fluorescence. Stain Tech. 34, 125–128.
McClure B.A., Haring V., Ebert P.R., Anderson M.A., Simpson R.J., Sakiyama F., Clarke A.E., 1989. Style self-incompatibility gene products of Nicotiana alata are ribonucleases. Nature 342, 955–957.
Milatović D., Nikolić D., 2007. Analysis of self-(in)compatibility in apricot cultivars using fluorescence microscopy. J. Hortic. Sci. Biotech. 82, 170–174.
Milatović D., Nikolić D., Rakonjac V., Fotirić Akšić M., 2010. Cross-incompatibility in apricot cultivars. J. Hort. Sci. Biotech. 85, 394–398.
Newbigin, E., Anderson, M.A., Clark, A.E., 1993. Gametophytic self-incompatibility systems. Plant Cell. 5, 1315–1324.
Nyújtó F., Brózik S. Jr., Nyéki J., 1985. Fruit set in apricot varieties. Acta Agron. Acad. Sci. Hung. 34, 65–72.
Paydas S., Eti S., Gülcan R., Derin K., Yilmaz K.U., 2006. In vitro investigations on pollen quality, production and self incompatibility of some apricot varieties in Malatya – Turkey. Acta Hortic. 701, 75–80.
Pedryc A., Kerek M.M., 1999. New apricot varieties in Hungary. Mitteil. Klostern. 49, 230–232.
Schultz J.H., 1948. Self-incompatibility in apricots. Proc. Am. Soc. Hortic. Sci. 51, 171–174.
Szabó Z., Nyéki J., 1991. Blossoming, fructification and combination of apricot varieties. Acta Hortic. 293, 295–302.
Topor E., Vasilescu R., Balan V., Tudor V., 2010. Apricot breeding programme for late and very late ripening period in Romania. Acta Hortic. 862, 137–142.
Viti R., Monteleone P., Guerriero R., 1997. Incompatibility in apricot (Prunus armeniaca L.): Methodological considerations. J. Hortic. Sci. 72, 961–970.
Zhang L., Chen X., Chen X., Zhang C., Liu X., Ci Z., Zhang H., Wu C., Liu C., 2008. Identification of self-incompatibility (S-) genotypes of Chinese apricot cultivars. Euphytica 160, 241–248.
Andrés M.V., Durán J.M., 1998. Self-incompatibility in Spanish clones of apricot (Prunus armeniaca L.) tree. Euphytica 101, 349–355.
Audergon J.M., Guerriero R., Monteleone P., Viti R., 1999. Contribution to the study of inheritance of the character self-incompatibility in apricot. Acta Hortic. 488, 275–280.
Badenes M.L., Llácer G., 2006. Breeding for resistance: breeding for Plum pox virus resistant apricots (Prunus armeniaca L.) in Spain. EPPO Bulletin 36, 323–326.
Badenes M.L., Hurtado M.A., Sanz F., Archelos D.M., Burgos L., Egea J., Llácer G., 2000. Searching for molecular markers linked to male sterility and self-compatibility in apricot. Plant Breed. 119, 157–160.
Benediková D., 2006. Apricot breeding in Slovak Republic for fruit quality and resistance to disease – New Slovak apricot cultivars created by RBS Veselé. Acta Hortic. 701, 377–380.
Burgos L., Egea J., Guerriero R., Viti R., Monteleone P., Audergon J.M., 1997. The self-compatibility trait of the main apricot cultivars and new selections from breeding programmes. J. Hortic. Sci. 72, 147–154.
Burgos L., Pérez-Tornero O., Ballester J., Olmos E., 1998. Detection and inheritance of stylar ribonucleases associated with incompatibility alleles in apricot. Sex. Plant Reprod. 11, 153–158.
De Nettancourt D., 1977. Incompatibility in angiosperms. Springer-Verlag, Berlin, Heidelberg, New York.
Drén G., Szabó Z., Thurzó S., Racskó J., Soltész M., Nyéki J., 2007. Experiences on the fertilization of foreign apricot cultivars. Intern. J. Hortic. Sci. 13(3), 143–145.
Egea J., Burgos L., 1996. Detecting cross-incompatibility of three North American apricot cultivars and establishing the first incompatibility group in apricot. J. Am. Soc. Hortic. Sci. 121, 1002–1005.
Egea J., Garcia J.E., Egea L., Berenguer T., 1991. Self-incompatibility in apricot cultivars. Acta Hortic. 293, 285–293.
Entani T., Iwano M., Shiba H., Che F.S., Isogai A., Takayama S., 2003. Comparative analysis of the self-incompatibility (S-) locus region of Prunus mume: identification of a pollen-expressed F-box gene with allelic diversity. Genes Cells 8, 203–213.
Gharesheikhbayat R., Dondini L., Sansavini S., 2011. Identification of self-incompatibility allels in apricot (Prunus armeniaca L.) using multi-level approaches. Seed Plant Impr. J. 27, 411–426.
Guerriero R., Bartolini S., 1995., Flower biology in apricot: main aspects and problems. Acta Hortic. 384, 261–271.
Hajilou J., Grigorian V., Mohammadi S.A., Nazemmieh A., Romero C., Vilanova S., Burgos L., 2006. Self- and cross-(in)compatibility between important apricot cultivars in northwest Iran. J. Hortic. Sci. Biotech. 81, 513–517.
Halász J., Hegedűs A., Hermán R., Stefanovits-Bányai É., Pedryc A., 2005. New selfincompatibility alleles in apricot (Prunus armeniaca L.) revealed by stylar ribonuclease assay and S-PCR analysis. Euphytica 145, 57–66.
Halász J., Pedryc A., Hegedűs A., 2007. Origin and dissemination of the pollen-part mutated SChaplotype that confers self-compatibility in apricot (Prunus armeniaca). New Phytol. 176, 792–803.
Halász J., Pedryc A., Ercisli S., Yilmaz K.U., Hegedűs A., 2010. S-genotyping supports the genetic relationships between Turkish and Hungarian apricot germplasm. J. Am. Soc. Hortic. Sci. 135, 410–417.
Jie Q., Shupeng G., Jixiang Z., Manru G., Huairui S., 2005. Identification of self-incompatibility genotypes of apricot (Prunus armeniaca L.) by S-allele-specific PCR analysis. Biotechnol. Lett. 27, 1205–1209.
Karayiannis I., 2006. Breeding for resistance: conventional breeding for Plum pox virus resistant apricots (Prunus armeniaca L.) in Greece. EPPO Bull. 36, 319–322.
Karayiannis I., Thomidis T., Tsaftaris A., 2008. Inheritance of resistance to Plum pox virus in apricot (Prunus armeniaca L.). Tree Genet. Genom. 4, 143–148.
Kostina K.F., 1970. Issledovanija po samoopyleniju abrikosa. Trudy Gosud. Nikit. Bot. Sada 45, 7–17.
Krška B., Salava J., Polák J., 2006a. Breeding for resistance: breeding for Plum pox virus resistant apricots (Prunus armeniaca L.) in the Czech Republic. EPPO Bull. 36, 330–331.
Krška B., Vachůn Z., Nečas T., 2006b. The apricot breeding programme at the Horticulture Faculty in Lednice. Acta Hortic. 717, 145–148.
Layne R.E.C., Bailey C.H., Hough L.F., 1996. Apricots. In: Fruit breeding. Vol. I: Tree and tropical fruits, Janick J., Moore, J.N. (eds.). John Wiley, Inc., New York, 79–111.
Ledbetter C.A., Ramming D.W., 1997. Apricot Cv. Robada. USA Plant Patent No. 9, 890.
Ledbetter C., Peterson S., Jenner J., 2006. Modification of sugar profiles in California adapted apricots (Prunus armeniaca L.) through breeding with Central Asian germplasm. Euphytica 148, 251–259.
Martin F.W., 1959. Staining and observing pollen tubes in the style by means of fluorescence. Stain Tech. 34, 125–128.
McClure B.A., Haring V., Ebert P.R., Anderson M.A., Simpson R.J., Sakiyama F., Clarke A.E., 1989. Style self-incompatibility gene products of Nicotiana alata are ribonucleases. Nature 342, 955–957.
Milatović D., Nikolić D., 2007. Analysis of self-(in)compatibility in apricot cultivars using fluorescence microscopy. J. Hortic. Sci. Biotech. 82, 170–174.
Milatović D., Nikolić D., Rakonjac V., Fotirić Akšić M., 2010. Cross-incompatibility in apricot cultivars. J. Hort. Sci. Biotech. 85, 394–398.
Newbigin, E., Anderson, M.A., Clark, A.E., 1993. Gametophytic self-incompatibility systems. Plant Cell. 5, 1315–1324.
Nyújtó F., Brózik S. Jr., Nyéki J., 1985. Fruit set in apricot varieties. Acta Agron. Acad. Sci. Hung. 34, 65–72.
Paydas S., Eti S., Gülcan R., Derin K., Yilmaz K.U., 2006. In vitro investigations on pollen quality, production and self incompatibility of some apricot varieties in Malatya – Turkey. Acta Hortic. 701, 75–80.
Pedryc A., Kerek M.M., 1999. New apricot varieties in Hungary. Mitteil. Klostern. 49, 230–232.
Schultz J.H., 1948. Self-incompatibility in apricots. Proc. Am. Soc. Hortic. Sci. 51, 171–174.
Szabó Z., Nyéki J., 1991. Blossoming, fructification and combination of apricot varieties. Acta Hortic. 293, 295–302.
Topor E., Vasilescu R., Balan V., Tudor V., 2010. Apricot breeding programme for late and very late ripening period in Romania. Acta Hortic. 862, 137–142.
Viti R., Monteleone P., Guerriero R., 1997. Incompatibility in apricot (Prunus armeniaca L.): Methodological considerations. J. Hortic. Sci. 72, 961–970.
Zhang L., Chen X., Chen X., Zhang C., Liu X., Ci Z., Zhang H., Wu C., Liu C., 2008. Identification of self-incompatibility (S-) genotypes of Chinese apricot cultivars. Euphytica 160, 241–248.
Dragan Milatović
University of Belgrade, Serbia
University of Belgrade, Serbia
Dragan Nikolić
University of Belgrade, Serbia
University of Belgrade, Serbia
Milica Fotirić-Akšić
University of Belgrade, Serbia
University of Belgrade, Serbia
Aleksandar Radović
University of Belgrade, Serbia
University of Belgrade, Serbia
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Autor podpisuje oświadczenie o oryginalności dzieła, wkładzie poszczególnych osób i źródle finansowania.
