Induction of adventitious buds and establishment of an efficient regeneration system in Polygonatum cyrtonema Hua
Yin-Kai Xi
Yunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Chinese Medicinal Materials, Yunnan University of Chinese Medicine, Kunming, China, 650000; Chemistry Department, Guizhou University of Traditional Chinese Medicine, Guiyang, China, 550000https://orcid.org/0000-0002-7515-5096
Min Yang
QingHong Meng
Hengyu Huang
Yunnan University of Chinese MedicineAbstract
Polygonatum cyrtonema Hua is not only a widely used traditional Chinese medicine, but also processed into food and cosmetics. However, its large-scale cultivation and application are limited by the low reproduction rate for traditional artificial propagation technology. Therefore, the aim of this study was to establish an efficient and stable adventitious buds induction system for P. cyrtonema by using one bud rhizome segment as initial explants. The results showed that the optimal medium for the rhizome growth, adventitious buds occurrence and proliferation was MS medium supplemented with 3.0 mg·L–1 BA, 1.0 mg·L–1 NAA and 1.0 mg·L–1 KT, with a proliferation coefficient of 6.5. The best rooting medium was found to be half strength of MS medium with 0.5–1.0 mg·L–1 NAA. The survival rate was higher than 95%. Finally, an asexual rapid propagation system of P. cyrtonema was established, which can provide the technical basis for the development of nursery culture.
Keywords:
adventitious buds, orthogonal experiment, Polygonatum cyrtonema, proliferation coefficient, rhizomeReferences
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Yunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Chinese Medicinal Materials, Yunnan University of Chinese Medicine, Kunming, China, 650000; Chemistry Department, Guizhou University of Traditional Chinese Medicine, Guiyang, China, 550000 https://orcid.org/0000-0002-7515-5096
Yunnan University of Chinese Medicine
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