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ASPHC Baner

Vol. 19 No. 2 (2020)

Articles

IMPACT OF VARIOUS 2, 4-D CONCENTRATIONS AND DIFFERENT VITAMIN MIXTURES ON in vitro CULTURE OF COCONUT (Cocos nucifera L.) BY UTILIZING SEEDLINGS SHOOT TIP

DOI: https://doi.org/10.24326/asphc.2020.2.13
Submitted: April 10, 2019
Published: 2020-04-24

Abstract

In this experiment, seedlings as sources for shoot tips was assembled from the coconut research institute (CRI) of Chinese Academy of tropical agricultural sciences germplasm, Wenchang, Hainan, China. The shoot tips were cultured on Y3 medium to study the impact of ten concentrations of 2, 4-D and three vitamin mixtures on survival, death and development % on in vitro culture establishment stage of coconut (Cocos nucifera L.). The obtained results revealed that all 2,4-D concentrations 4,7, 75, 100, 120 and 140 mg/L + any vitamin mixture did not give any development and high concentrations of 2, 4-D increased the death %. In any case, it can be concluded that the rate of differences in survival, death and development percentages exhibited by 2, 4-D concentrations was more pronounced than the analogous ones resulted by vitamin mixtures. At any rate, utilizing of (2, 4-D at concentration 25 mg/L+ vitamin mixture 1 or 2) exhibited the greatest values of survival percentage and reduced death percentage. Just as, increased the percentages of development in in vitro culture establishment stage of coconut (Cocos nucifera L.) through seedlings shoot tip.

References

  1. Amoo, S.O., Staden, J.V. (2013). Influence of plant growth regulators on shoot proliferation and secondary metabolite production in micro propagated Huernia hystrix. Plant Cell Tissue Organ Cult., 112, 249–256.
  2. Batugal, P., Rao, R.V., Oliver, J. (2005). Coconut genetic resources. International Plant Genetic Resources Institute Regional Office for Asia, the Pacific and Oceania (IPGRI-APO). Serdang,
  3. Córdova-Lara, I., Chan-Rodríguez, J.L., Sandoval-Cancino, G., Rivera-Solis, G., Oropeza-Salín, C. (2016). Somatic Embryogenesis in Cocos nucifera L. In: Somatic embryogenesis: fundamental aspects and applications, Loyola-Vargas, V.M., Ochoa-Alejo, N. (eds.). Springer International Publishing, Switzerland, 297–318.
  4. Ebert, A., Taylor, H.F. (1990). Assessment of the changes of 2,4-dichlorophenoxyacetic acid concentrations in plant tissue culture media in the presence of activated charcoal. Plant Cell Tissue Organ Cult., 20, 165–172.
  5. Eeuwens, C.J. (1976). Mineral requirements for growth and callus initiation of tissue explants excised from mature coconut (Cocos nucifera) and date (Phoenix dactylifera) palms cultured in vitro. Physiol. Plant., 36, 23–28.
  6. Fehér, A., Pasternak, T.P., Dudits, D. (2003). Transition of somatic plant cells to an embryogenic state. Plant Cell Tissue Organ Cult., 74(3), 201–228. DOI: 10.1023/A:1024033216561
  7. Hills, J.F., Thomas, L. (1978). Statistical Methods in Agriculture Research, 3rd ed. Univ. California publishers, USA.
  8. Hornung, R., Verdeil, J.L. (1999). Somatic embryogenesis in coconut from immature inflorescence.
  9. Jemenez, V.M., Bangerth, F. (2001). Hormonal status of maize initial explants and of the embryogenic and non-embryogenic callus cultures derived from them as related to morphogenesis in vitro, Plant Sci., 160(2), 247–257.
  10. Lao, D.A. (2009). Coco-biodiesel in the Philippines. In: Coconut Philippines published by Asia.
  11. Lebon, G., Duchene, E., Brun, O., Magne, C., Clement, C. (2004). Flower abscission and inflorescence carbohydrates in sensitive and non-sensitive cultivars of grapevine. Sex Plant Reprod., 17, 71–9.
  12. Meziani, R., Jaiti, F., Mazri, M.A., Anjarne, M., Chitt, M.A., Fadile, J.E., Alem, C. (2015). Effects of plant growth regulators and light intensity on the micropropagation of date palm (Phoenix dactylifera L.) cv. Mejhoul. J. Crop Sci. Biotech., 18(5), 325–331.
  13. Michalczuk, L., Ribnicky, D.M., Cooke, T.J., Cohen, J.D. (1992). Regulation of indole-3-acetic acid biosynthetic pathways in carrot cell cultures, Plant Physiol., 100(3), 1346–1353.
  14. Ohira, K., Makoto, I., Ojima, K. (1976). Thiamine requirements of various plant cells in suspension culture. Plant Cell Physiol., 17(3), 583–590.
  15. Ozyigit, I.I. (2008). Phenolic changes during in vitro organogenesis of cotton (Gossypium hirsutum L.) shoot tips. Afr. J. Biotechnol., 7(8), 1145–1150.
  16. Pasternak, T., Miskolczi, P., Ayaydin, F., Mészáros, T., Dudits, D., Fehér, A. (2000). Exogenous auxin and cytokinin dependent activation of CDKs and cell division in leaf protoplast-derived cells of alfalfa, Plant Growth Regul., 32, 2–3, 129–141.
  17. Perera, P.I., Hocher, V., Verdeil, J.L., Doulbeau, S., Yakandawala, D.M., Weerakoon, L.K. (2007). Unfertilized ovary: A novel explant for coconut (Cocos nucifera L.) somatic embryogenesis. Plant Cell Rep., 26(1), 21–28. DOI: 10.1007/s00299-006-0216-4
  18. Roolant, L. (2014). Why coconut water is now a one billion industry. Available: https://transferwise.com/blog/2014-05/why-coconut-water-is-now-a-1-billionindustry/ [date of access: 2.11.2015].
  19. Sáenz, L., Oropeza, C., Chan, J.L., Hornungm R., Rillo, E., Verdeil, J.L. (1999). The use of plumular explants for the regeneration of coconut. In: Current advances in coconut biotechnology, Oropeza, C., Verdeil, J.L., Ashburner, G.R., Cardeña, R., Santamaría, J. (eds.). Kluwer Academic Publishers, The Netherlands, 309–319.
  20. Torres, K.C. (1989). Tissue culture techniques for horticultural crops. Chapman and Hall, New York–London.
  21. Vasil, I.K., Thorpe, T.A. (1998). Plant cell and tissue culture. Kluwer Acad. Publ., Dordrecht.
  22. Verdeil, J.L., Buffard-Morel, J., Pannetier, C. (1989). Somatic embryogenesis of coconut (Cocos nucifera L.) from leaf and inflorescence tissue. Research findings and prospects. Oleagineux, 44, 403–411.
  23. Verdeil, J.L., Huet, C., Grosdemange, F., Buffard-Morel, J. (1994). Plant regeneration from cultured immature inflorescences of coconut (Cocos nucifera L.): evidence for somatic embryogenesis. Plant Cell Rep., 13, 218–221.
  24. Vidhanaarachchi, V.R., Fernando, S.C., Perera, P.I., Weerakoon, L.K. (2013). Application of un-fertilized ovary culture to identify elite mother palms of Cocos nucifera L. with regenerative potential. J. Nation. Sci. Found. Sri Lanka, 41(1), 29–34. DOI: 10.4038/jnsfsr.v41i1.5329
  25. Weerakoon, L.K. (2004). Coconut tissue and embryo culture in Sri Lanka: current developments and future challenges. In: Proceedings of international conference Coconut Research Institut Sri Lanka. Part 1 (Review papers and guest presentations). CRI, Lunuwila, Sri Lanka, 41–61.
  26. White, P.R. (1943). Nutrient deficiency studies and improved inorganic nutrients for cultivation of excised tomato roots. Growth, 7, 53– 65.
  27. Zaman, M.A., Manjur, A.B.M.K., Ahmed, M., Islam, M.M. (2010). Effect of 2,4-D on callus induction and subsequent morphogenesis in mature chickpea (Cicerarietinum L.) embryo culture. Tissue Cult. Biotechnol., 7(9), 53–58.

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