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Vol. 18 No. 2 (2019)

Articles

HARDENING PRETREATMENT BY DROUGHT AND LOW TEMPERATURE ENHANCED CHILLING STRESS TOLERANCE OF CUCUMBER SEEDLINGS

DOI: https://doi.org/10.24326/asphc.2019.2.4
Submitted: April 12, 2019
Published: 2019-04-12

Abstract

Chilling stress is of major limiting factors influencing the growth and development of warm-season crops like cucumber. In this research, the possibility of chilling tolerance of cucumber seedlings was investigated through employing the drought and low-temperature pretreatments. The factorial experiment consisted of two factors including cucumber cultivars (i.e. ‘Super Dominos’ and ‘Super Star’) and hardening treatments (control, low temperatures at 10°C, and 15°C and drought simulated by 10% and 20% PEG) based on completely randomized design (CRD) in 3 replications. After applying treatments and providing them 48 h opportunity to be recovered, the seedlings were subjected to 3°C for a six-day period and 6 h for each day. All hardening treatments improved seedlings’ growth, chlorophyll content, total phenol (TP) and antioxidant enzyme activities, while reducing chilling injury index and malondialdehyde (MDA) content. Comparing to temperature hardening, the drought pretreatment showed to have a better effect on inducing the chilling tolerance into cultivars. Overall, the results of this experiment showed that employing drought and low-temperature pretreatments enabled cucumber seedlings to mitigate the harmful effects of chilling.

References

  1. Allen, D.J., Ort, D.R. (2001). Impacts of chilling temperatures on photosynthesis in warm-climate plants. Trends Plant Sci., 6(1), 36–42.
  2. Aron, D.I. (1949). Copper enzymes isolated chloroplasts, polyphenoloxidase in Beta vulgaris. Plant Physiol., 24, 1–15.
  3. Anjum, N.A. (2015). Book review: oxidative damage to plants-antioxidant networks and signaling. Front. Plant Sci., 6, 452.
  4. Ao, P.X., Li, Z.G., Fan, D.M., Gong, M. (2013). Involve-ment of antioxidant defense system in chill hardening-induced chilling tolerance in Jatropha curcas seedlings. Acta Physiol. Plant., 35(1), 153–160.
  5. Berova, M., Zlatev, Z., Stoeva, N. (2002). Effect of paclobutrazol on wheat seedlings under low temperature stress. Bulg. J. Plant Physiol., 28(1–2), 75–84.
  6. Cayuela, E., Muñoz‐Mayor, A., Vicente‐Agulló, F., Moyano, E., Garcia‐Abellan, J.O., Estañ, M.T., Bolarín, M.C. (2007). Drought pretreatment increases the salinity resistance of tomato plants. J. Plant Nut. Soil Sci., 170(4), 479–484.
  7. Dat, J., Vandenabeele, S., Vranová, E., Van Montagu, M., Inzé, D., Van Breusegem, F. (2000). Dual action of the active oxygen species during plant stress responses. Cell Mol. Life Sci., 57(5), 779–795.
  8. Dong, X., Bi, H., Wu, G., Ai, X. (2013). Drought-induced chilling tolerance in cucumber involves membrane stabilisation improved by antioxidant system. Int. J. Plant Prod., 7(1), 67–80.
  9. Gong, M., Chen, B.O., Li, Z.G., Guo, L.H. (2001). Heat-shock-induced cross adaptation to heat, chilling, drought and salt stress in maize seedlings and involvement of H2O2. J. Plant Physiol., 158(9), 1125–1130.
  10. Helmy, Y.I., Singer, S.M., El-Abd, S.O. (1997). Reducing chilling injury by short-term cold acclimation of cucumber seedlings under protected cultivation. International Symposium Greenhouse Management for Better Yield and Quality in Mild Winter Climates, 491, 177–184.
  11. Hossain, M.A., Burritt, D.J., Fujita, M. (2016). Cross-stress tolerance in plants: molecular mechanisms and possible involvement of reactive oxygen species and methylglyoxal detoxification systems. Abiotic Stress Response Plants, 323–375.
  12. Hura, K., Hura, T., Rapacz, M., Pƚażek, A. (2015). Effects of low-temperature hardening on the biochemical response of winter oilseed rape seedlings inoculated with the spores of Leptosphaeria maculans. Biologia, 70(8), 1011–1018.
  13. Kang, H.M., Saltveit, M.E. (2001). Activity of enzymatic antioxidant defense systems in chilled and heat shocked cucumber seedling radicles. Physiol. Plant., 113(4), 548–556.
  14. Korkmaz, A., Dufault, R.J. (2001). Developmental consequences of cold temperature stress at transplanting on seedling and field growth and yield. I. Watermelon. J. Am. Soc. Hortic. Sci., 126(4), 404–409.
  15. Li, H.Y., Li, C.G., Gong, M. (2011). Short-term cold-shock at 1 C induced chilling tolerance in maize seedlings. Int. Confer. Biol. Environ. Chem., 1, 346–349.
  16. Li, X., Cai, J., Liu, F., Dai, T., Cao, W., Jiang, D. (2014). Physiological, proteomic and transcriptional responses of wheat to combination of drought or waterlogging with late spring low temperature. Funct. Plant Biol., 41(7), 690–703.
  17. Maali-Amiri, R., Goldenkova-Pavlova, I.V., Yur’Eva, N.O., Pchelkin, V.P., Tsydendambaev, V.D., Veresh-chagin, A.G., Nosov, A.M. (2007). Lipid fatty acid composition of potato plants transformed with the Δ12-desaturase gene from cyanobacterium. Russ. J. Plant Physiol., 54(5), 600–606.
  18. Mahajan, S., Tuteja, N. (2005). Cold, salinity and drought stresses: an overview. Arch. Biochem. Biophys., 444(2), 139–158.
  19. Mei, Y.Q., Song, S.Q. (2010). Response to temperature stress of reactive oxygen species scavenging enzymes in the cross-tolerance of barley seed germination. J. Zhejiang Univ. Sci. B., 11(12), 965–972.
  20. Nayyar, H., Bains, T.S., Kumar, S. (2005). Chilling stressed chickpea seedlings: effect of cold acclimation, calcium and abscisic acid on cryoprotective solutes and oxidative damage. Environ. Exp. Bot., 54(3), 275–285.
  21. Pardossi, A., Tognoni, F., Lovemore, S.S. (1987). The effect of different hardening treatments on tomato seedling growth, chilling resistance and crop production in cold greenhouse. Symposium on Biological Aspects of Energy Saving in Protected Cultivation, 229, 371–378.
  22. Plewa, M.J., Smith, S.R., Wagner, E.D. (1991). Diethyl-dithiocarbamate suppresses the plant activation of aromatic amines into mutagens by inhibiting tobacco cell peroxidase. Mutat. Res. Fundam. Mol. Mech. Mutagen., 247(1), 57–64.
  23. Rice-Evans, C., Miller, N., Paganga, G. (1997). Antioxi-dant properties of phenolic compounds. Trends Plant Sci., 2(4), 152–159.
  24. Saltveit, M.E. (2001). Chilling injury is reduced in cucumber and rice seedlings and in tomato pericarp discs by heat-shocks applied after chilling. Postharvest Biol. Technol., 21(2), 169–177.
  25. Schütz, M., Fangmeier, A. (2001). Growth and yield responses of spring wheat (Triticum aestivum L. cv. Minaret) to elevated CO2 and water limitation. Environ. Pollut., 114(2), 187–194.
  26. Singleton, V.L., Rossi, J.A. (1965). Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enology Vitic., 16(3), 144–158.
  27. Song, Y., Diao, Q., Qi, H. (2014). Putrescine enhances chilling tolerance of tomato (Lycopersicon esculentum Mill.) through modulating antioxidant systems. Acta Physiol. Plant., 36(11), 3013–3027.
  28. Stewart, R.R., Bewley, J.D. (1980). Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiol., 65(2), 245–248.
  29. Streb, P., Aubert, S., Gout, E., Feierabend, J., Bligny, R. (2008). Cross tolerance to heavy-metal and cold-induced photoinhibiton in leaves of Pisum sativum acclimated to low temperature. Physiol. Mol. Biol. Plant., 14(3), 185.
  30. Szőllősi, R. (2014). Superoxide dismutase (SOD) and abiotic stress tolerance in plants: An overview. In: Oxidative Damage to Plants, Ahmad, P. (ed.). Elsevier, San Diego, 89–129.
  31. Tewari, A.K., Tripathy, B.C. (1998). Temperature-stress-induced impairment of chlorophyll biosynthetic reactions in cucumber and wheat. Plant Physiol., 117(3), 851–858.
  32. Yu, C.W., Murphy, T.M., Sung, W.W., Lin, C.H. (2002). H2O2 treatment induces glutathione accumulation and chilling tolerance in bean. Funct. Plant Biol., 29(9), 1081–1087.

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