Abstrakt
The aim of the study was to evaluate cellulolytic and amylolytic enzymes activities in substrates, regarding the time of the Pleurotus ostreatus (Mori Hiratake no 39)
B7 mycelium incubation, and the stage of physiological development. The experiment was divided into three parts which had different times of mycelium incubation before fructification: I – the time of incubation – 2 weeks; II – 4 weeks; III – 6 weeks. After the mycelium incubation, cold-shock and mechanical-shock was provided on each series. Researches proved that agents such as the time of mycelium incubation in substrate, agrotechnical treatment (cold-shock, mechanical-shock), and the type of mycelium layer had an impact on cellulolytic and amylolytic activity of mycelium in the cultivation’s substrate. The biggest influence on change of enzymatic activity had agrotechnical treatment: cold-shock and mechanical-shock.
Bibliografia
Baldrian P., Valášková V., Merhautová V., Gabriel J., 2005. Degradation of lignocellulose by Pleurotus ostreatus in the presence of copper, manganese, lead and zinc. Res Microbiol., 156, 670–676.
Carroll G.C., Wicklow D.T., 1992. The Fungal Community. Its organization and role in the ecosystem. 2nd ed Marcel Dekker. NJ New York.
De Groot P.W.J., Visser J., Van Griensver L.J.L.D., Schaap P.J., 1998. Biochemical and molecular aspects of grown and fruiting of the edible mushroom Agaricus bisporus. Mycol Res., 102, 1297–1308.
Dundar A., Acay H., Yildiz A., 2009. Effect of using different lignocellulosic wastes for cultivation of Pleurotus ostreatus (Jacq.) P. Kumm. on mushroom yield, chemical composition and nutritional value. African J. Biotechnol.. 8, 4, 662–666.
Elisashvili V., Parlar H., Kachlishvili E., Chichua D., Bakradze M., Kohreidze N., 2001. Ligninolytic activity of basidiomycetes grown under submerged and solid-state fermentation on plant raw material (sawdust of grapevine cuttings). Adv. Food Sci., 23, 117–23.
Eriksson K.E., Blanchette R.A., Ander P., 1990. Microbial and enzymatic degradation of wood and wood components. [In:] Springer Series in Wood Science., Springer-Verlag Berlin.
Galhaup C., Wagner H., Hinterstoisser B., Haltrich D., 2002. Increased production of laccase by the wood-degrading basidiomycete Trametes pubescens. Enzyme Microb Technol., 30, 529–536.
Hadar Y., Kerem Z., Gorodecki B., Ardon O., 1992. Utilization of lignocellulosic waste by the edible mushroom, Pleurotus. Biodegradation, 3, 189–205.
Hoff J.A., Klopfenstein N.B., McDonald G.I., Tonn, J.R., Kim M.S., Zambino P.J., Hessburg P.F., Rogers J.D., Peever T.L., Carris L.M., 2004. Fungal endophytes in woody roots of Douglas-fir (Pseudotsuga menziesii) and ponderosa pine (Pinus ponderosa). Forest Pathology, 34, 255–271.
Kirk T.K., Farrell R.L., 1987. Enzymatic combustion: the microbial degradation of lignin. Annu Rev Microbiol., 41, 465–505.
Manzi P., Gambelli L., Marconi S., Vivanti V., Pizzoferrato L., 1999. Nutrients in edible mushrooms: An inter-species comparative study. Food Chem. 65, 4, 477–482.
Mikiashvili N., Wasser S., Nevo E., Chichua D., Elisashvili V., 2004. Lignocellulolytic enzyme activities of medicinally important basidiomycetes from different ecological niches. Int. J. Med. Mushr., 6, 63–71.
Miller G.L., 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal Chem., 31, 426–428.
Moldes D., Lorenzo M., Sanroman M. A., 2004. Different proportion of laccase isoenzymes produced by submerged cultures of Trametes versicolor grown on lignocellulosic wastes. Biotechnol Lett., 26, 327–230.
Ohga S., Smith M., Trurston C., Wood D.A., 1999. Transcriptional regulation of laccase and cellulase genes in the mycelium of Agaricus bisporus during fruit body development on a solid substrate. Mycol. Res., 103, 1557–1560.
Ohga S., Cho N. S., Thurston C. F., Wood D.A., 2000. Transcriptional regulation of laccase and cellulase in relation to fruit body formation in the mycelium of Lentinula edodes on a sawdustbased substrate. Mycoscience, 41, 149–153.
Omoanghe I.S., Mikiashvili N.A., 2009. Lignocellulolytic enzyme activity, substrate utilization, and mushroom yield by Pleurotus ostreatus cultivated on substrate containing anaerobic digester solids. J. Ind. Microbiol. Biotechnol., 36, 1353–1362.
Singh A., Abduilah N., Vikineswary S., 2003. Optimization of extraction of bulk enzymes from spent mushroom compost. J. Chem. Technol. Biotechnol., 78, 743–752.
Tshinyangu K. K., 2006. Effect of grass hay substrate on nutritional value of Pleurotus ostreatis var. columbinus. Nahrung-Food, 40, 79–83.
Valmaseda M., Martinez M.J., Martinez A., 1991. Kinetics of wheat straw solid-state fermentation with Trametes versicolor and Pleurotus ostreatus – lignin and polysaccharide alteration and production of related enzymatic activities. Appl. Microbiol. Biotechnol., 35, 817–823.
Vikineswary S., Abdullah N., Renuvathani M., Sekaran M., Pandey A., Jones E.B.G., 2006.
Productivity of laccase in solid substrate fermentation of selected agro-residues by Pycnoporus sanguineus. Bioresour. Technol., 97, 171–177.
Wang D., Sakoda A., Suzuki M., 2001. Biological efficiency and nutritional value of Pleurotus ostreatus cultivated on spent beer grain. Bioresour. Technol., 78, 293–300.
Downloads
Download data is not yet available.
