Przejdź do głównego menu Przejdź do sekcji głównej Przejdź do stopki
ASPHC Baner

Tom 21 Nr 6 (2022)

Artykuły

In vitro propagation of Gypsophila pilulifera, an endangered endemic ornamental plant species

DOI: https://doi.org/10.24326/asphc.2022.6.2
Przesłane: 9 marca 2022
Opublikowane: 2022-12-30

Abstrakt

Gypsophila pilulifera Boiss. & Heldr., an endemic and endangered species is an economically important plant that has a potential used in medicine thank to the saponins it contains. It is also an ornamental plant. In this study, the production was carried out under in vitro conditions in order to protect and economically evaluate the species, which is in danger of extinction due to its distribution in a very narrow area open to anthropogenic effects. For this purpose, seed and shoot tip explants of G. pilulifera were cultured in Murashige and Skoog (MS) medium containing different concentrations of 6-benzylaminopurine (BAP), indole-3-acetic acid (IAA) and naphthaleneacetic acid (NAA). Explants were obtained from two different locations of the natural habitats of G. pilulifera and more plants were obtained in the tissue culture from both explant types taken from the 1st location. Propagation from seed gave better results, and 96.67% of the seeds cultured in hormone-free MS medium were germinated and rooted. The shoot tip showed lower regeneration in vitro than the seed explant. Especially the rooting percentage is quite low and the highest rooting and shoot formation was obtained in MS medium containing 2 mg/L BAP + 2 mg/L IAA. In this medium, 10.50 ±0.17 shoots were obtained per plant, and rooting (1.67 ±0.09 per plant) was also achieved only in this medium. Seedlings obtained in tissue culture continue to develop successfully in field conditions, and this method has been determined as a suitable method for ex-situ conservation and economically rapid production of the species.

Bibliografia

  1. Ahroni, A., Zuker A., Rozen, Y., Shejtman, H., Vainstein A. (1997). An efficient method for adventitious shoot regeneration from stem-segment explants of Gypsophila. Plant Cell Tissue Organ Cult., 49(2), 101–106. https://doi.org/10.1023/A:1005806627306 DOI: https://doi.org/10.1023/A:1005806627306
  2. Arslan, I., Celik, A., Chol, J.H. (2012). A cytotoxic triterpenoid saponin from under-ground parts of Gypsophila pilulifera Boiss. & Heldr. Fitoterapia, 83(4), 699–703. https://doi.org/10.1016/j.fitote.2012.02.005 DOI: https://doi.org/10.1016/j.fitote.2012.02.005
  3. Chima, N.K., Nahar, L., Majinda, R.R., Celik, S., Sarker, S.D. (2014). Assessment of free-radical scavenging activity of Gypsophila pilulifera: assay-guided isolation of verbascoside as the main active component. Rev. Bras. Farmacogn., 24(1), 38–43. https://doi.org/10.1590/0102-695X20142413391 DOI: https://doi.org/10.1590/0102-695X20142413391
  4. Copaci, C.M., Szatmari, P.M., Mladin, L., Sicora, O., Sicora, C. (2018). Gypsophila collina – an endangered species ex situ conserved in “Vasile Fatiˮ botanical garden jibou. Acta Med. Marisiensis, 64(3), 10.
  5. Delay, C., Gavin, J.A., Aumelas, A., Bonnet, P.A., Roumestand, C. (1997). Isolation and structure elucidation of a highly haemolytic saponin from the Merck saponin extract using high-field gradient-enhanced NMR techniques. Carbohydr. Res., 302, 67–78. https://doi.org/10.1016/s0008-6215(97)00101-8 DOI: https://doi.org/10.1016/S0008-6215(97)00101-8
  6. Gera, A., Weintraub, P.G., Maslenin, L., Spiegel, S., Zeidan, M. (2007). A new disease causingstunting and shoot proliferation in Gypsophila is associated with phytoplasma.Bull. Insectol., 60(2), 271–272.
  7. Gevrenova, R., Stancheva, T., Voynikov, Y., Laurain-Mattar, D., Henry, M. (2010). Root in vitro cultures of six Gypsophila species and their saponin contents. Enzyme Microb. Technol., 47(3), 97–104. https://doi.org/10.1016/j.enzmictec.2010.05.007 DOI: https://doi.org/10.1016/j.enzmictec.2010.05.007
  8. Gribble, K. (1999). The influence of relative humidity on vitrification, growth and morphology of Gypsophila paniculata L. Plant Growth Regul., 27(3), 181–190. DOI: https://doi.org/10.1023/A:1006235229848
  9. Hostettmann, K., Marston, A. (1995). Saponins. In: Chemistry and pharmacology of natural products, Phillipon, J.D., Ayres, D.C., Baxter. H. (eds). Cambridge University Press, Cambridge. https://doi.org/10.1017/CBO9780511565113 DOI: https://doi.org/10.1017/CBO9780511565113
  10. Isik, M., Korkmaz, M., Bursal, E., Gulcin, I., Koksal, E., Tohma, H. (2015). Determination of antioxidant properties of Gypsophila bitlisensis Bark. Int. J. Pharmacol., 11(4), 366–37l. https://doi.org/10.3923/ijp.2015.366.371 DOI: https://doi.org/10.3923/ijp.2015.366.371
  11. Kapchina-Toteva, V., Stoyanova, D. (2003). Effect of cytokinins and cytokinin antagonists on in vitro cultured Gypsophila paniculata L. Biol. Plant., 46(3), 337–341. https://doi.org/10.1023/A:1024357614990 DOI: https://doi.org/10.1023/A:1024357614990
  12. Kaya, A.S., Karaguzel, O., Aydinşakir, K., Kazaz, S., Ozçelik, A. (2012). Usage possibilities of some Gypsophila species naturally grown in Türkiye as ornamental plants. Derim, 29(1), 37–47.
  13. Muca, B. (2017). Türkiye Gypsophila L. (Caryophyllaceae) Cinsine Ait CapituliformesWilliams Seksiyonunun Revizyonu (in Turkish) [Türkiye Gypsophila L. – Revision of the Capilliformis Williams Section of the Genus]. PhD thesis, Suleyman Demirel University, Isparta, Türkiye.
  14. Murashige, T., Skoog, F. (1962). A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiol. Plant., 15, 473–497. DOI: https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  15. Rady, M. (2006). In vitro culture of Gypsophila paniculata L. and random amplified polymorphic DNA analysis of the propagated plants. Biol. Plant., 50, 507–513. https://doi.org/10.1007/s10535-006-0080-7 DOI: https://doi.org/10.1007/s10535-006-0080-7
  16. Rady, M., Bekheet, S. (2008). Effect of temperature, sugar and vessel closure on in vitro growth of Gypsophila paniculata. Floricult. Ornam. Biotechnol., 2(2), 73–76.
  17. Rahman, A.U. (2002). Bioactive natural products. Elsvier Publications, Amsterdam, Netherlands, Vol. 26.
  18. Rashid, S., Ilyas, S., Naz, S., Aslam, F., Ali, A. (2012). In vitro propagation of Gypsophila paniculata L. through plant tissue culture techniques. Pak. J. Sci., 64(1), 6–10.
  19. Shillo, R. (1985). Gypsophila paniculata. In: Handbook of flowering, Halevy, A.H. (ed.), vol. 3. CRC, Boca Raton, Florida, 83–87.
  20. Simeonova, R., Vitcheva, V., Kondeva-Burdina, M., Krasteva, I., Manov, V., Mitcheva, M. (2013). Hepatoprotective and antioxidant effects of saponarin, isolated from Gypsophila trichotoma Wend. on paracetamol-induced liver damage in rats. BioMed Res. Int., Article ID 757126. https://doi.org/10.1155/2013/757126 DOI: https://doi.org/10.1155/2013/757126
  21. Zuker, A., Ahroni, A., Shejtman, H., Vainstein, A. (1997). Adventitious shoot regeneration from leaf explants of Gypsophila paniculata L. Plant Cell Rep., 16(11), 775–778. https://doi.org/10.1007/s002990050318 DOI: https://doi.org/10.1007/s002990050318

Downloads

Download data is not yet available.

Podobne artykuły

<< < 4 5 6 7 8 9 10 11 12 13 > >> 

Możesz również Rozpocznij zaawansowane wyszukiwanie podobieństw dla tego artykułu.