Rozmnażanie storczyków w kulturach tkankowych
PAWEŁ SZOT
Katedra Roślin Ozdobnych i Architektury Krajobrazu, Uniwersytet Przyrodniczy w Lublinie ul. Głęboka 28, 20-612 LublinMARZENA PARZYMIES
Katedra Roślin Ozdobnych i Architektury Krajobrazu, Uniwersytet Przyrodniczy w Lublinie ul. Głęboka 28, 20-612 LublinMONIKA PONIEWOZIK
Katedra Roślin Ozdobnych i Architektury Krajobrazu, Uniwersytet Przyrodniczy w Lublinie ul. Głęboka 28, 20-612 LublinAbstrakt
Storczyki to jedne z najważniejszych roślin ozdobnych. W związku z ogromną liczbą gatunków i odmian storczyków, z których większość nie może być rozmnażana generatywnie, rośliny te są rozmnażane także wegetatywnie. Jedną z metod wykorzystywaną powszechnie jest rozmnażanie in vitro. W pracy przedstawiono istniejący stan wiedzy na temat kiełkowania nasion w warunkach in vitro oraz mikrorozmnażania storczyków, z uwzględnieniem najbardziej znaczących wyników badań.
Słowa kluczowe:
mikrorozmnażanie, PLB, protokormy, orchidea, kalus, nasionaBibliografia
Aktar S., Nasiruddin K.M., Khaldun A.B.M., 2007. Organogenesis of Dendrobium Orchids Using Traditional Media and Organic Extracts. J. Agric. Rural Dev. 5, 30–35.
Asghar S., Ahmad T., Hafiz I.A., Yaseen M., 2011. In vitro propagation of orchid (Dendrobium nobile) var. Emma white [!]. Afr. J. Biotechnol. 10 (16), 3097–3103.
Avila-Diaz I., Oyama K., Gomez-Alonso C., Salgado-Gaeciglia R., 2009. In vitro propagation of endangered orchids Laelia speciosa. Plant Cell Tiss. Organ Cult. 99, 335–343.
Balilashaki K., Naderi R., Kalantari S., Soorni A., 2014. Micropropagation of Phalaenopsis amabilis cv. ‘Cool Breeze’ with using of folwer stalk nodes and leaves of sterile obtained from node cultures. Int. J. Farm. Alli. Sci. 3 (7), 823–829.
Bhadra S.K., Hossain M.M., 2003. In vitro germination and micropropagation of Geodorum densiflorum (Lam.) Schltr., an endangered orchid species. Plant Tiss. Cult. 13, 165–171.
Chang W.C., Chang J.T., Chun Chen Y., Lin Y.H., Su Y.H., Chen T.Y, Tseng M.C, Kuo H.L., Wu I.F, Chueh C.M., 2005. In Vitro Morphogenesis of Five Orchids. Acta Hortic. 692, 115–118.
Cha-um S., Ulziibat B., Kirdmanee Ch., 2010. Effects of temperature and relative humidity during in vitro acclimatization, on physiological changes and growth character of Phalaenopsis adapted to in vivo. Am. J. Cosmet. Surg. 4 (9), 750–756.
Chen T.-Y., Chen J.-T., Chang W.-C., 2004. Plant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum orchids. Plant Cell Tiss. Organ Cult. 76, 11–15.
Collins M.T., Dixon K.W., 1992. Micropropagation of an Australian terrestrial orchid Diuris longifolia R. Br. Austral. J. Exp. Agric. 32, 131–135.
Dohling S., Kumaria S., Tandon P., 2012. Multiple shoot induction from axillary bud cultures of the medicinal orchid, Dendrobium longicornu. AoB Plants, doi: 10:1093/aobpla/pls032.
Fakouri Ghaziani M.V., Baker A., Negahdar N., Kaviani B., 2014. Micropropagation of Orchis catasetum – a rare and endangered orchid. Sci. Papers. Ser. B, Horticulture 58, 317–322.
Geetha S., Shetty S.A., 2000. In vitro propagation of Vanilla planifolia a tropical orchid. J. Curr. Sci. 79, 886–889.
Giridhar P., Ravishabkar G.A., 2003. Efficient micropropagation of Vanilla planifolia Andr. under influence of thidiazuron, zeatin and coconut milk. Indian J. Biotechnol. 3, 113–118.
Gnasekaran P., Poobathy R., Mahmood M., Samian M.R., Subramaniam S., 2012. Effects of complex organic additives on improving the growth of PLBs of Vanda Kasem’s Delight. Aust. J. Crop Sci. 6 (8), 1245–1248.
Hajong S., Kumaria S., Tandon P., 2013. Effect of plant growth regulators on regeneration potential of axenic nodal segments od Dendrobium chrysanthum Wall. Ex Lindl. J. Agric. Sci. Technol. 15, 1425–1435.
Hossain M.M., 2008. Asymbiotic seed germination and in vitro seedling development of Epidendrum ibaguense Kunth. (Orchidaceae). Afr. J. Biotechnol. 7, 3614–3619.
Hossain M.M., Sharma M., Teixeira da Silva J.A., Pathak P., 2010. Seed germination and tissue culture of Cymbidium giganteum Wall. ex Lindl. Sci. Hortic. 123, 479–487.
Huang L.-Ch., Lin Ch.-J., Kuo Ch.-I., Huang B.-L., Murashige T., 2001. Paphiopedilum cloning in vitro. Sci. Hortic. 91, 111–121.
Ishii Y., Takamura T., Goi M., Tanaka M., 1998. Callus induction and somatic embriogenesis of Phalaenopsis. Plant Cell Rep. 17, 446–450.
Islam M.N., Nasiruddin K.M., Al-Amin M., 2014. In vitro growth and multiplication of a hybrid orchid (Dendrobium alba × Ascanda dongtarm) with different concentration of plant growth regulators. J. Biosci. Agric. Res. 1, 27–33.
Kalimuthu K., Senthilkumar R., Vijayakumar S., 2007. In vitro microporpagation of orchid, Oncidium sp. (Dancing Dolls). Afr. J. Biotechnol. 6, 1171–1174.
Kannan N., 2009. An in vitro study on micropropagation od Cymbidium orchids. Curr. Biot. 23, 244–250.
Khatun H., Khatun M.M., Biswas M.S., Kabir M.R., Al-Amin M., 2010. In vitro growth and development od Dendrobium hybrid orchid. Bangladesh J. Agril Res. 35 (3), 507–514.
Knudson L., 1922. Non-symbiotic germination of orchid seeds. Bot. Gaz. 73, 1–25.
Kosir P., Skof S., Luthar Z., 2004. Direct shoot regeneration from nodes of Phalaenopsis Orchids. Acta Agric. Slov. 83 (2), 233–242.
Kukułczanka K., Kromer K., 1984. Namnażanie i rozwój protokormów Phalaenopsis w kulturach in vitro. Acta Univ. Wratisl. Pr. Bot. 667 (30), 15–29.
Kukułczanka K., Paluch B., 1971. Zastosowanie peptonu Peptobak-Bacutil w hodowli merystematycznej tkanki Cymbidium Sw. Acta Agrobot. 24 (1), 53–62.
Kumar A., Nandi S.K., Bag N., Palni L.M.S., 2002. Tissue culture studies in two important orchid taxa: Rhynchostylis retusa (L.) Bl. and Cymbidium elegans Lindl. Gyanodaya Prakashan, Nainital, India, 113–124.
Lee Y-I., Hsu S-T., Yeung E.C., 2013. Orchid protocorm-like bodies are somatic embryos. Am. J. Bot. 100 (11), 1–11, DOI: 10.3732/ajb.1300193.
Liao Y.-J., Tsai Y.-Ch., Sun Y.-W., Lin R.-S., Wu F.-S., 2011. In vitro shoot induction and plant regeneration from flower buds in Paphiopedilum orchids. In Vitro Cell. Dev. Biol. Plant 47, 702–709.
Malabadi R.B., Mulgund G.S., Kallappa N., 2004. Micropropagation of Dendrobium nobile from shoot tips sections. J. Plant Physiol. 162, 473–478.
Mathews V.H., Rao P.S., 1985. In vitro Culture of Vanda Hybrid (Vanda TMA × Vanda Miss Joaquim). II. Studies on Seedling Explants. Proc. Indian Nat. Sci. Acad. 4, 496–504.
Mohapatra A., Rout G.R., 2005. In vitro micropropagation of Geoderum purpureum R. Br. Indian J. Biotechnol. 4, 568–570.
Moraes L., Faria R.T., Cuquel F.L., 2005. Activated charcoal for in vitro propagation of Brazilian orchids. Acta Hortic. 683 (3), 383–389.
Murashige T., Skoog F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–479.
Murdad R., Hwa K.S., Seng C.K., Latip M.A., Aziz Z.A., Ripin R., 2006. High frequency multiplication of Phalaenopsis gigantea using trimmed bases protocoms techinique. Sci. Hortic. 111, 73–79.
Naing A.H., Chung J.D., Lim K.B., 2011. Plant Regeneration trough Indirect Somatic Embryogenesis in Coelogyne Cristata Orchid. Am. J. Plant Sci. 2, 262–267.
Nambiar N., Tee C.S., Maziah M., 2012. Effects of organic additives and different carbohydrate sources on proliferation of protocorm-like bodies in Dendrobium Alya Pink. Plant Omics J. 5 (1), 10–18.
Nayak N.R., Patnaik S., Rath S.P., 1997. Direct shoot regeneration from foliar explants of an epiphytic orchid Acampe praemorsa (Roxb.) Blatter and McCann. Plant Cell Rep. 16, 583–586.
Nge K.L., Nwe N., Chandrkrachang S., Stevens W.F., 2006. Chitosan as a growth stimulator in orchid tissue culture. Plant Sci. 170, 1185–1190.
Niknejad A., Kadir M.A., Kadzimin S.B., 2011. In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroidae: Orchideaceae). Afr. J. Biotechnol. 10 (56), 11808–11816.
Nontachaiyapoom T.S., Sasirat S., Manoch L., 2010 Isolation and identification of Rhizoctonia-like fungi from roots of three orchid genera, Paphiopedilum, Dendrobium, and Cymbidium, collected in Chiang Rai and Chiang Mai provinces of Thailand. Mycorrhiza 20, 459–471.
Oszkinis K., 1991. Storczyki. PWRiL, Warszawa.
Pacek-Bieniek A., Dyduch-Siemińska M., Rudaś M., 2010. Influence of activated charcoal on seed germination and seedling development by asymbiotic method in Zygostates grandiflora (Lindl.) Mansf. (Orchideaceae). Folia Hortic. 22 (2), 45–50.
Pedroza-Manrique J., Fernandez-Lizarazo Ch., Suarez-Silva A., 2005. Evaluation of the effect of three growth regulators in the germination of Comparettia falcata seeds under in vitro conditions. In Vitro Cell. Dev. Biol., Plant 41, 838–843.
Pindel A., Pindel Z., 2004. Initiation of in vitro cultures of chosen endangered European species of orchids. Folia Hortic. 16 (2), 111–117.
Puchooa D., 2004. Comparsion of different culture media for the in vitro culture Dendrobium (Orchideaceae). Int. J. Agric. Biol. 6, 884–888.
Pyati A.N., Murthy H.N., Hahn E.J., Paek K.Y., 2002. In vitro propagation of Dendrobium macrostachyum Lindl. – A threatened orchid. Indian J. Exp. Biol. 40, 620–623.
Ramesh T., Sabariraj K., Nithiya R., Naveenraj D., Krubha J., Chitra B., Renganathan P., 2013. In vitro studies and conservation of endangered orchids in Phalaenopsis species. Int. J. Cur. Tr. Res. 2 (1), 97–99.
Rasmussen H., Andersen T.F., Johansen B., 1990. Light stimulation and darkness requirement for symbiotic germination of Dactylorhiza majalis (Orchideace) in vitro. Physiol. Plant. 79, 226–230.
Valle Rego-Olivera L. do, Faria R.T. de, 2005. In vitro propagation of Brazilian orchids using traditional culture media and commercial fertilizers formulations. Acta Scient. Agron. 27, 1–5.
Roy J., Banerjee N., 2003. Induction of callus and plant regeneration from shoot-tip explants of Dendrobium fimbriatum Lindl. var. oculatum Hk. F. Sci. Hortic. 97, 333–340.
Santana G.E., Chaparro K., 1999. Clonal propagation of Oncidium through the culture of floral buds. Acta Hortic. 482, 315–320.
Sharma V., 2012. Orchid micropropagation: regeneration competence of anther culture. J. Biotechnol. Biomater. 2, 149, doi: 10.4172/2155-952X.1000149.
Talukder S.K., Nasiruddin K.M., Yasmin S., Hassan L., Begum R., 2003. Shoot proliferation of Dendrobium Orchid with BAP and NAA. J. Biol. Sci. 3 (11), 1058–1062.
Tee Ch.S., Lee P.S., Kiong A.L.P., Mahmood M., 2010. Optimisation of protoplast isolation protocols using in vitro leaves of Dendrobium crumenatum (pigeon orchid). Afr. J. Agric. Res. 5 (19), 2685–2693.
Teixeira da Silva J.A., 2012. New basal media for protocorm-like body and callus induction of hybrid Cymbidium. J. Fruit Ornam. Plant Res. 20 (2), 127–133.
Teixeira da Silva J.A., Giang D.T.T., 2014. Unsuccessful in vitro regeneration from Phalaenopsis (Orchideaceae) flowers. All Res. J. Biol. 5, 18–22.
Tokuhara K., Mii M., 2003. Highly – efficient somatic embriogenesis from cell suspension cultures of Phalaenopsis orchid by adjusting carbohydrate sources. In Vitro Cell. Dev. Biol., Plant 39, 365–639.
Tonecki J., Dobrzyński P., 2008. Rozmnażanie storczyka europejskiego Platanthera bifolia (L.) L. C. Rich. w warunkach in vitro. Zesz. Probl. Postęp. Nauk Rol. 525, 419–425.
Udomdee W., Wen P.-J., Chin S.-W., Chen F.-Ch., 2012. Shoot multiplication of Paphiopedilum orchid through in vitro cutting methods. Afr. J. Biotechnol. 11 (76), 14077–14082.
Waes J.V., 1987. Effect of activated charcoal on in vitro propagation of western european orchids. Acta Hortic. 212 (1), 131–138.
Warghat A.R., Bajpai P.K., Srivastava R.B., Chaurasia O.P., Chauhan R.S., Sood H., 2014. In vitro protocorm development and mass multiplication of an endangered orchid, Dactyloriza hatagirea. Turk. J. Bot. 38, 737–746
Wróblewska W., Rudzki P., 2012. Tendencje w produkcji roślin ozdobnych metodą kultur tkankowych w Polsce i na świecie. Annales UMCS, Horticultura 22 (4), 20–27.
Wu J., Ma H., Lü M., Han S., Zhu Y., Jin H., Liang J., Liu L., Xu J., 2010. Rhizoctonia fungi enhance the growth of the endangered orchid Cymbidium goeringii. Botany 88, 20–29.
Yam T.W., Arditti J., 2009. History of orchid propagation: a mirror of the history of biotechnology. Plant Biotechnol. Rep. 3, 1–56.
Zaniecka J., Łojkowska E., 2004. Estabilishment of in vitro culture collection of endangered European orchids. Biul. Ogrod. Bot. Muz. Zbior. 13, 69–73.
Zettler L.W., McInnis T.M., 1994. Light enhancement of symbiotic seed germination and development of an endangered terrestrial orchid (Platanthera integrilabia). Plant Sci. 102, 133–138.
Asghar S., Ahmad T., Hafiz I.A., Yaseen M., 2011. In vitro propagation of orchid (Dendrobium nobile) var. Emma white [!]. Afr. J. Biotechnol. 10 (16), 3097–3103.
Avila-Diaz I., Oyama K., Gomez-Alonso C., Salgado-Gaeciglia R., 2009. In vitro propagation of endangered orchids Laelia speciosa. Plant Cell Tiss. Organ Cult. 99, 335–343.
Balilashaki K., Naderi R., Kalantari S., Soorni A., 2014. Micropropagation of Phalaenopsis amabilis cv. ‘Cool Breeze’ with using of folwer stalk nodes and leaves of sterile obtained from node cultures. Int. J. Farm. Alli. Sci. 3 (7), 823–829.
Bhadra S.K., Hossain M.M., 2003. In vitro germination and micropropagation of Geodorum densiflorum (Lam.) Schltr., an endangered orchid species. Plant Tiss. Cult. 13, 165–171.
Chang W.C., Chang J.T., Chun Chen Y., Lin Y.H., Su Y.H., Chen T.Y, Tseng M.C, Kuo H.L., Wu I.F, Chueh C.M., 2005. In Vitro Morphogenesis of Five Orchids. Acta Hortic. 692, 115–118.
Cha-um S., Ulziibat B., Kirdmanee Ch., 2010. Effects of temperature and relative humidity during in vitro acclimatization, on physiological changes and growth character of Phalaenopsis adapted to in vivo. Am. J. Cosmet. Surg. 4 (9), 750–756.
Chen T.-Y., Chen J.-T., Chang W.-C., 2004. Plant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum orchids. Plant Cell Tiss. Organ Cult. 76, 11–15.
Collins M.T., Dixon K.W., 1992. Micropropagation of an Australian terrestrial orchid Diuris longifolia R. Br. Austral. J. Exp. Agric. 32, 131–135.
Dohling S., Kumaria S., Tandon P., 2012. Multiple shoot induction from axillary bud cultures of the medicinal orchid, Dendrobium longicornu. AoB Plants, doi: 10:1093/aobpla/pls032.
Fakouri Ghaziani M.V., Baker A., Negahdar N., Kaviani B., 2014. Micropropagation of Orchis catasetum – a rare and endangered orchid. Sci. Papers. Ser. B, Horticulture 58, 317–322.
Geetha S., Shetty S.A., 2000. In vitro propagation of Vanilla planifolia a tropical orchid. J. Curr. Sci. 79, 886–889.
Giridhar P., Ravishabkar G.A., 2003. Efficient micropropagation of Vanilla planifolia Andr. under influence of thidiazuron, zeatin and coconut milk. Indian J. Biotechnol. 3, 113–118.
Gnasekaran P., Poobathy R., Mahmood M., Samian M.R., Subramaniam S., 2012. Effects of complex organic additives on improving the growth of PLBs of Vanda Kasem’s Delight. Aust. J. Crop Sci. 6 (8), 1245–1248.
Hajong S., Kumaria S., Tandon P., 2013. Effect of plant growth regulators on regeneration potential of axenic nodal segments od Dendrobium chrysanthum Wall. Ex Lindl. J. Agric. Sci. Technol. 15, 1425–1435.
Hossain M.M., 2008. Asymbiotic seed germination and in vitro seedling development of Epidendrum ibaguense Kunth. (Orchidaceae). Afr. J. Biotechnol. 7, 3614–3619.
Hossain M.M., Sharma M., Teixeira da Silva J.A., Pathak P., 2010. Seed germination and tissue culture of Cymbidium giganteum Wall. ex Lindl. Sci. Hortic. 123, 479–487.
Huang L.-Ch., Lin Ch.-J., Kuo Ch.-I., Huang B.-L., Murashige T., 2001. Paphiopedilum cloning in vitro. Sci. Hortic. 91, 111–121.
Ishii Y., Takamura T., Goi M., Tanaka M., 1998. Callus induction and somatic embriogenesis of Phalaenopsis. Plant Cell Rep. 17, 446–450.
Islam M.N., Nasiruddin K.M., Al-Amin M., 2014. In vitro growth and multiplication of a hybrid orchid (Dendrobium alba × Ascanda dongtarm) with different concentration of plant growth regulators. J. Biosci. Agric. Res. 1, 27–33.
Kalimuthu K., Senthilkumar R., Vijayakumar S., 2007. In vitro microporpagation of orchid, Oncidium sp. (Dancing Dolls). Afr. J. Biotechnol. 6, 1171–1174.
Kannan N., 2009. An in vitro study on micropropagation od Cymbidium orchids. Curr. Biot. 23, 244–250.
Khatun H., Khatun M.M., Biswas M.S., Kabir M.R., Al-Amin M., 2010. In vitro growth and development od Dendrobium hybrid orchid. Bangladesh J. Agril Res. 35 (3), 507–514.
Knudson L., 1922. Non-symbiotic germination of orchid seeds. Bot. Gaz. 73, 1–25.
Kosir P., Skof S., Luthar Z., 2004. Direct shoot regeneration from nodes of Phalaenopsis Orchids. Acta Agric. Slov. 83 (2), 233–242.
Kukułczanka K., Kromer K., 1984. Namnażanie i rozwój protokormów Phalaenopsis w kulturach in vitro. Acta Univ. Wratisl. Pr. Bot. 667 (30), 15–29.
Kukułczanka K., Paluch B., 1971. Zastosowanie peptonu Peptobak-Bacutil w hodowli merystematycznej tkanki Cymbidium Sw. Acta Agrobot. 24 (1), 53–62.
Kumar A., Nandi S.K., Bag N., Palni L.M.S., 2002. Tissue culture studies in two important orchid taxa: Rhynchostylis retusa (L.) Bl. and Cymbidium elegans Lindl. Gyanodaya Prakashan, Nainital, India, 113–124.
Lee Y-I., Hsu S-T., Yeung E.C., 2013. Orchid protocorm-like bodies are somatic embryos. Am. J. Bot. 100 (11), 1–11, DOI: 10.3732/ajb.1300193.
Liao Y.-J., Tsai Y.-Ch., Sun Y.-W., Lin R.-S., Wu F.-S., 2011. In vitro shoot induction and plant regeneration from flower buds in Paphiopedilum orchids. In Vitro Cell. Dev. Biol. Plant 47, 702–709.
Malabadi R.B., Mulgund G.S., Kallappa N., 2004. Micropropagation of Dendrobium nobile from shoot tips sections. J. Plant Physiol. 162, 473–478.
Mathews V.H., Rao P.S., 1985. In vitro Culture of Vanda Hybrid (Vanda TMA × Vanda Miss Joaquim). II. Studies on Seedling Explants. Proc. Indian Nat. Sci. Acad. 4, 496–504.
Mohapatra A., Rout G.R., 2005. In vitro micropropagation of Geoderum purpureum R. Br. Indian J. Biotechnol. 4, 568–570.
Moraes L., Faria R.T., Cuquel F.L., 2005. Activated charcoal for in vitro propagation of Brazilian orchids. Acta Hortic. 683 (3), 383–389.
Murashige T., Skoog F., 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15, 473–479.
Murdad R., Hwa K.S., Seng C.K., Latip M.A., Aziz Z.A., Ripin R., 2006. High frequency multiplication of Phalaenopsis gigantea using trimmed bases protocoms techinique. Sci. Hortic. 111, 73–79.
Naing A.H., Chung J.D., Lim K.B., 2011. Plant Regeneration trough Indirect Somatic Embryogenesis in Coelogyne Cristata Orchid. Am. J. Plant Sci. 2, 262–267.
Nambiar N., Tee C.S., Maziah M., 2012. Effects of organic additives and different carbohydrate sources on proliferation of protocorm-like bodies in Dendrobium Alya Pink. Plant Omics J. 5 (1), 10–18.
Nayak N.R., Patnaik S., Rath S.P., 1997. Direct shoot regeneration from foliar explants of an epiphytic orchid Acampe praemorsa (Roxb.) Blatter and McCann. Plant Cell Rep. 16, 583–586.
Nge K.L., Nwe N., Chandrkrachang S., Stevens W.F., 2006. Chitosan as a growth stimulator in orchid tissue culture. Plant Sci. 170, 1185–1190.
Niknejad A., Kadir M.A., Kadzimin S.B., 2011. In vitro plant regeneration from protocorms-like bodies (PLBs) and callus of Phalaenopsis gigantea (Epidendroidae: Orchideaceae). Afr. J. Biotechnol. 10 (56), 11808–11816.
Nontachaiyapoom T.S., Sasirat S., Manoch L., 2010 Isolation and identification of Rhizoctonia-like fungi from roots of three orchid genera, Paphiopedilum, Dendrobium, and Cymbidium, collected in Chiang Rai and Chiang Mai provinces of Thailand. Mycorrhiza 20, 459–471.
Oszkinis K., 1991. Storczyki. PWRiL, Warszawa.
Pacek-Bieniek A., Dyduch-Siemińska M., Rudaś M., 2010. Influence of activated charcoal on seed germination and seedling development by asymbiotic method in Zygostates grandiflora (Lindl.) Mansf. (Orchideaceae). Folia Hortic. 22 (2), 45–50.
Pedroza-Manrique J., Fernandez-Lizarazo Ch., Suarez-Silva A., 2005. Evaluation of the effect of three growth regulators in the germination of Comparettia falcata seeds under in vitro conditions. In Vitro Cell. Dev. Biol., Plant 41, 838–843.
Pindel A., Pindel Z., 2004. Initiation of in vitro cultures of chosen endangered European species of orchids. Folia Hortic. 16 (2), 111–117.
Puchooa D., 2004. Comparsion of different culture media for the in vitro culture Dendrobium (Orchideaceae). Int. J. Agric. Biol. 6, 884–888.
Pyati A.N., Murthy H.N., Hahn E.J., Paek K.Y., 2002. In vitro propagation of Dendrobium macrostachyum Lindl. – A threatened orchid. Indian J. Exp. Biol. 40, 620–623.
Ramesh T., Sabariraj K., Nithiya R., Naveenraj D., Krubha J., Chitra B., Renganathan P., 2013. In vitro studies and conservation of endangered orchids in Phalaenopsis species. Int. J. Cur. Tr. Res. 2 (1), 97–99.
Rasmussen H., Andersen T.F., Johansen B., 1990. Light stimulation and darkness requirement for symbiotic germination of Dactylorhiza majalis (Orchideace) in vitro. Physiol. Plant. 79, 226–230.
Valle Rego-Olivera L. do, Faria R.T. de, 2005. In vitro propagation of Brazilian orchids using traditional culture media and commercial fertilizers formulations. Acta Scient. Agron. 27, 1–5.
Roy J., Banerjee N., 2003. Induction of callus and plant regeneration from shoot-tip explants of Dendrobium fimbriatum Lindl. var. oculatum Hk. F. Sci. Hortic. 97, 333–340.
Santana G.E., Chaparro K., 1999. Clonal propagation of Oncidium through the culture of floral buds. Acta Hortic. 482, 315–320.
Sharma V., 2012. Orchid micropropagation: regeneration competence of anther culture. J. Biotechnol. Biomater. 2, 149, doi: 10.4172/2155-952X.1000149.
Talukder S.K., Nasiruddin K.M., Yasmin S., Hassan L., Begum R., 2003. Shoot proliferation of Dendrobium Orchid with BAP and NAA. J. Biol. Sci. 3 (11), 1058–1062.
Tee Ch.S., Lee P.S., Kiong A.L.P., Mahmood M., 2010. Optimisation of protoplast isolation protocols using in vitro leaves of Dendrobium crumenatum (pigeon orchid). Afr. J. Agric. Res. 5 (19), 2685–2693.
Teixeira da Silva J.A., 2012. New basal media for protocorm-like body and callus induction of hybrid Cymbidium. J. Fruit Ornam. Plant Res. 20 (2), 127–133.
Teixeira da Silva J.A., Giang D.T.T., 2014. Unsuccessful in vitro regeneration from Phalaenopsis (Orchideaceae) flowers. All Res. J. Biol. 5, 18–22.
Tokuhara K., Mii M., 2003. Highly – efficient somatic embriogenesis from cell suspension cultures of Phalaenopsis orchid by adjusting carbohydrate sources. In Vitro Cell. Dev. Biol., Plant 39, 365–639.
Tonecki J., Dobrzyński P., 2008. Rozmnażanie storczyka europejskiego Platanthera bifolia (L.) L. C. Rich. w warunkach in vitro. Zesz. Probl. Postęp. Nauk Rol. 525, 419–425.
Udomdee W., Wen P.-J., Chin S.-W., Chen F.-Ch., 2012. Shoot multiplication of Paphiopedilum orchid through in vitro cutting methods. Afr. J. Biotechnol. 11 (76), 14077–14082.
Waes J.V., 1987. Effect of activated charcoal on in vitro propagation of western european orchids. Acta Hortic. 212 (1), 131–138.
Warghat A.R., Bajpai P.K., Srivastava R.B., Chaurasia O.P., Chauhan R.S., Sood H., 2014. In vitro protocorm development and mass multiplication of an endangered orchid, Dactyloriza hatagirea. Turk. J. Bot. 38, 737–746
Wróblewska W., Rudzki P., 2012. Tendencje w produkcji roślin ozdobnych metodą kultur tkankowych w Polsce i na świecie. Annales UMCS, Horticultura 22 (4), 20–27.
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PAWEŁ SZOT
Katedra Roślin Ozdobnych i Architektury Krajobrazu, Uniwersytet Przyrodniczy w Lublinie ul. Głęboka 28, 20-612 Lublin
Katedra Roślin Ozdobnych i Architektury Krajobrazu, Uniwersytet Przyrodniczy w Lublinie ul. Głęboka 28, 20-612 Lublin
MARZENA PARZYMIES
Katedra Roślin Ozdobnych i Architektury Krajobrazu, Uniwersytet Przyrodniczy w Lublinie ul. Głęboka 28, 20-612 Lublin
Katedra Roślin Ozdobnych i Architektury Krajobrazu, Uniwersytet Przyrodniczy w Lublinie ul. Głęboka 28, 20-612 Lublin
MONIKA PONIEWOZIK
Katedra Roślin Ozdobnych i Architektury Krajobrazu, Uniwersytet Przyrodniczy w Lublinie ul. Głęboka 28, 20-612 Lublin
Katedra Roślin Ozdobnych i Architektury Krajobrazu, Uniwersytet Przyrodniczy w Lublinie ul. Głęboka 28, 20-612 Lublin
Licencja
Przesyłając artykuł do publikacji, autor oświadcza, że posiada pełnię autorskich praw majątkowych oraz osobistych do utworu, a jego opublikowanie nie naruszy praw osób trzecich. Autor podpisuje oświadczenie o oryginalności dzieła i wkładzie poszczególnych osób.
Przesłanie przez autora artykułu do publikacji w czasopiśmie „Annales Horticulturae” traktowane będzie jako udzielenie licencji do eksploatacji praw autorskich do artykułu na zasadach licencji Creative Commons Uznanie autorstwa - Użycie niekomercyjne - Bez utworów zależnych 4.0 (CC BY-NC-ND 4.0)
