POSSIBILITY OF ACHIEVING ORGANIC YIELDS FOR MEDICINAL AND AROMATIC PLANTS BY BIOFERTILIZATION WITH Azotobacter chroococcum


Abstract

The aim of this study was to examine the effects of management practices and biofertilization on microbial
activity in rhizosphere and yield of medicinal and aromatic plants. Field experiment was performed using
four plant species: peppermint (Mentha × piperita L.), pot marigold (Calendula officinalis L.), sweet basil
(Ocimum basilicum L.), and dill (Anethum graveolens L.). Treatments were arranged in a split-plot layout in
four replicates using basic plots under conventional and organic management, and subplots with and without
biofertilizer (Azotobacter chroococcum). Organic management positively affected the microbial number and
activity. Biofertilization increased the total microbial number (13–21%), number of ammonifiers (13–60%),
nitrogen-fixing bacteria (7–36%), actinomycetes (36–50%), fungi (60–100%), cellulolytic microorganisms
(57–217%), dehydrogenase (28–52%) and ß-glucosidase activity (15–39%). The effects of management
practices and biofertilization were highly significant for the yield of examined plants. The yields were higher
on inoculated treatments both in conventional (5–26%) and organic (7–15%) growing system.


Keywords

Azotobacter chroococcum; dill; peppermint; pot marigold; sweet basil

El-Hadi, N.I.M.A., El-Ala, H.K.A., El-Azim, W.M.A. (2009). Response of some Mentha species to plant growth promoting bacteria (PGPB) isolated from soil rhizosphere. Aust. J. Basic Appl. Sci., 3, 4437–4448.

Béguin, P., Aubert, J.P. (1994). The biological degradation of cellulose. FEMS Microbiol. Rev., 13, 25–58.

Berg, G., Smalla, K. (2009). Plant species and soil type co¬operatively shape the structure and function of microbial communities in the rhizosphere. FEMS Microbiol. Ecol., 68, 1–13. DOI: 10.1111/j.1574-6941.2009.00654.x

Bouizgarne, B. (2013). Bacteria for plant growth promotion and disease management. In: Bacteria in agrobiology: disease management, Maheshwari, D. (ed.). Springer, Berlin−Heidelberg, 15–45. DOI: 10.1007/978-3-642- 33639-3_2

Briones, A.M., Reichardt, W. (1999). Estimating microbial population counts by ‘most probable number’ using Mi¬crosoft Excel. J. Microbiol. Meth., 35, 157–161.

Cardoso, E.J.B.N., Vasconcellos, R.L.F., Bini, D., Miyauu¬chi, M.Y.H., dos Santos, C.A., Alves, P.R.L., de Paula, A.M., Nakatani, A.S., de Moraes Pereira, J., Nogueira, M.A. (2013). Soil health: looking for suitable indicators. What should be considered to assess the effects of use and management on soil health? Sci. Agric., 70, 274– 289. DOI: 10.1590/S0103-90162013000400009

Casida, L.E.J., Klein, D.A., Santoro, T. (1964). Soil dehy¬drogenase activity. Soil Sci., 98, 371–376.

Darzi, M.T. (2012). Influence of organic fertilizer and bio¬stimulant on the growth and biomass of dill (Anethum graveolens). Intl. J. Agri. Crop Sci., 4, 98–102.

Gomiero, T., Pimentel, D., Paoletti, M.G. (2011). En¬vironmental impact of different agricultural man¬agement practices: conventional vs. organic ag¬riculture. Crit. Rev. Plant Sci., 30, 95–124. DOI: 10.1080/07352689.2011.554355

Hayano, K. (1973). A method for the determination of ß-glucosidase activity in soil. Soil Sci. Plant Nutr., 19, 103–108.

Heidari, G., Khosro, M., Sohrabi, Y. (2016). Responses of soil microbial biomass and enzyme activities to tillage and fertilization systems in soybean (Glycine max L.) production. Front. Plant Sci., 7, 1730. DOI: 10.3389/ fpls.2016.01730

Hosseinzadah, F., Satei, A., Ramezanpour, M.R. (2011). Effects of mycorrhiza and plant growth promoting rhi¬zobacteria on growth, nutrient uptake and physiological characteristics in Calendula officinalis L. Middle East J. Sci. Res., 8, 947–953.

IUSS Working Group WRB. (2014). World Reference Base for Soil Resources 2014. International Soil Classifica¬tion System for Naming Soils and Creating Legends for Soil Maps. World Soil Resources Reports No. 106. FAO, Rome, 181.

Jnawali, A.D., Ojha, R.B., Marahatta, M. (2015). Role of Azotobacter in soil fertility and sustainability – A re¬view. Adv. Plants Agric. Res., 2, 00069.

Kaschuk, G., Alberton, O., Hungria, M. (2010). Three de¬cades of soil microbial biomass studies in Brazilian eco¬systems: lessons learned about soil quality and indica¬tions for improving sustainability. Soil Biol. Biochem., 42, 1–13. DOI: 10.1016/j.soilbio.2009.08.020

Köberl, M., Schmidt, R., Ramadan, E.M., Bauer, R., Berg, G. (2013). The microbiome of medicinal plants: diversity and importance for plant growth, quality, and health. Front. Microbiol., 4, 400. DOI: 10.3389/ fmicb.2013.00400

Lamsal, K., Kim, S.W., Kim, Y.S., Lee, Y.S. (2013). Biocon¬trol of late blight and plant growth promotion in tomato using rhizobacterial isolates. J. Microbiol. Biotechnol., 23, 897–904. DOI: 10.4014/jmb.1209.09069

Liang, C., Balser, T.C. (2011). Microbial production of re¬calcitrant organic matter in global soils: implications for productivity and climate policy. Nat. Rev. Microbiol., 9, 75. DOI: 10.1038/nrmicro2386-c1

Mendes, R., Garbeva, P., Raaijmakers, J.M. (2013). The rhizosphere microbiome: significance of plant bene¬

Bjelić, D., Adamović, D., Marinković, J., Tintor, B., Mrkovački, N. (2019). Possibility of achieving organic yields for medicinal and aromatic plants by biofertilization with Azotobacter chroococcum. Acta Sci. Pol. Hortorum Cultus, 18(5), 3–11. DOI: 10.24326/ asphc.2019.5.1

ficial, plant pathogenic, and human pathogenic micro¬organisms. FEMS Microbiol. Rev., 37, 634–663. DOI: 10.1111/1574-6976.12028

Mrkovački, N., Milić, V. (2001). Use of Azotobacter croo¬coccum as potentially usefull in agrixultural application. Ann. Microbiol., 51, 145–159.

Ordookhani, K., Sharafzadeh, S., Zare, M. (2011). Influence of PGPR on growth, essential oil and nutrients uptake of sweet basil. Adv. Environ. Biol., 5, 672–677.

Qasim, M., Abideen, Z., Adnan, M.Y., Gulzer, S., Gul, B., Rasheed, M., Khan, M.A. (2017). Antioxidant proper-ties, phenolic composition, bioactive compounds and nutritive value of medicinal halophytes commonly used as herbal teas. S. Afr. J. Bot., 110, 240–250. DOI: 10.1016/j.sajb.2016.10.005

Qi, X., Wang, E., Xing, M., Zhao, W., Chen, X. (2012). Rhizosphere and nonrhizosphere bacterial community composition of the wild medicinal plant Rumex pati¬entia. World J. Microbiol. Biotechnol., 28, 2257–2265. DOI: 10.1007/s11274-012-1033-2

Seufert, V., Ramankutty, N., Foley, J.A. (2012). Comparing the yields of organic and conventional agriculture. Na¬ture, 485, 229–232. DOI: 10.1038/nature11069

Sharma, S., Gupta, R., Dugar, G., Srivastava A.K. (2012). Impact of application of biofertilizers on soil structure and resident microbial community structure and func¬tion. In: Bacteria in agrobiology: plant probiotics, Ma-heshwari, D. (ed.). Springer, Berlin, Heidelberg, 65–77. DOI: 10.1007/978-3-642-27515-9_4

Solaiman, Z.M., Anawar, H.Md. (2015). Rhizosphere microbes interactions in medicinal plants. In: Plant growth promoting rhizobacteria (PGPR) and medicinal plants, Egamberdieva, D., Shrivastava, S., Varma, S. (eds). Springer International Publishing, 19–41. DOI: 10.1007/978-3-319-13401-7_2

Teixeira da Silva, J.A., Egamberdieva, D. (2013). Plant-growth promoting rhizobacteria and medicinal plants. In: Recent progress in medicinal plants. Vol. 38. Es¬sential Oils III and Phytopharmacology, Govil, J.N., Bhattacharya, S. (eds). Studium Press LLC, Houston, 26–42.

Vacheron, J., Desbrosses, G., Bouffaud, M.L., Touraine, B., Loccoz, Y.M., Muller, D., Legendre, L., Wisniewski-Dyé, F., Prigent-Combaret, C. (2013). Plant growth-promot¬ing rhizobacteria and root system functioning. Front. Plant Sci., 4, 356. DOI: 10.3389/fpls.2013.00356

Wani, S.A., Chand, S., Ali, T. (2013). Potential use of Azoto¬bacter chroococcum in crop production: an overview. Curr. Agric. Res., 1, 35–38. DOI: 10.12944/CARJ.1.1.04

Wolinska, A., Stepniewska, Z. (2012). Dehydrogenase ac¬tivity in the soil environment. In: Dehydrogenases, Ca-nuto, R.A. (ed.). InTech, 183–210. DOI: 10.5772/4829411
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Published : 2019-10-28


Bjelić, D., Adamović, D., Marinković, J., Tintor, B., & Mrkovački, N. (2019). POSSIBILITY OF ACHIEVING ORGANIC YIELDS FOR MEDICINAL AND AROMATIC PLANTS BY BIOFERTILIZATION WITH Azotobacter chroococcum. Acta Scientiarum Polonorum Hortorum Cultus, 18(5), 3-11. https://doi.org/10.24326/asphc.2019.5.1

Dragana Bjelić  dragana.bjelic@ifvcns.ns.ac.rs
Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia  Serbia
Dušan Adamović 
Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia  Serbia
Jelena Marinković 
Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia  Serbia
Branislava Tintor 
Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia  Serbia
Nastasija Mrkovački 
Institute of Field and Vegetable Crops, 21000 Novi Sad, Serbia  Serbia


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