PHYSIOLOGICAL AND BIOCHEMICALS CHANGES MODULATED BY SEEDS’ PRIMING OF LENTIL (Lens culinaris L.) UNDER SALT STRESS AT GERMINATION STAGE
Amal Bouallègue
Laboratoire des Légumineuses, Centre de Biotechnologie de Borj Cedria (CBBC), PB 901, 2050, Hammam Lif, TunisieFatma Souissi
Laboratoire des Légumineuses, Centre de Biotechnologie de Borj Cedria (CBBC), PB 901, 2050, Hammam Lif, TunisieIssam Nouairi
Laboratoire des Légumineuses, Centre de Biotechnologie de Borj Cedria (CBBC), PB 901, 2050, Hammam Lif, TunisieMonia Souibgui
Laboratoire des Légumineuses, Centre de Biotechnologie de Borj Cedria (CBBC), PB 901, 2050, Hammam Lif, TunisieZouhaier Abbes
Laboratoire des Grandes Cultures, Institut National de la Recherche Agronomique de Tunisie (INRAT), Université de Carthage, Rue Hédi Karray, 2080 Ariana, TunisieHaythem Mhadhbi
Laboratoire des Légumineuses, Centre de Biotechnologie de Borj Cedria (CBBC), PB 901, 2050, Hammam Lif, TunisieAbstract
Seed priming is one of the potential physiological approaches to enhance seed germination under salt stress. The present study examined the role of two seed priming molecules, salicylic acid (SA) and hydrogen peroxide (H2O2), to enhance the salt tolerance in lentil seeds at germination stage. Salt stress caused significant decrease in germination percentage and primary root elongation. This decrease was associated with significant increase in lipid peroxidation and total lipid (TL) contents in embryonic axis. The catalase (CAT), guaiacol peroxydase (GPOX) and superoxide dismutase (SOD) activities remained unchanged or decreased significantly under the influence of salt stress, in both embryonic axis and cotyledons. Starch mobilization was not affected by salt stress. The two priming treatments effectively alleviated the negative effects of salt stress. SA and H2O2 applications after dose optimization resulted in significant enhancement of germination percentage and primary root elongation. No significant changes in starch, soluble sugars contents and SOD activity were detected following SA and H2O2 treatments. Seed priming treatments triggered the activities of GPOX and CAT and caused the reduction of lipid peroxidation especially in embryonic axis. TL content and especially the fatty acid C18:3 increased after SA applications. The better performance under salt stress of primed lentil seeds was associated with lower lipid peroxidation, and activation of enzymatic antioxidative defense system. Obtained results confirm the potential for using SA and H2O2 to improve germination and plant growth under salt stress conditions.
Keywords:
salt, seeds priming, Lens culinaris, antioxidant, fatty acids, germination, starchReferences
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Laboratoire des Légumineuses, Centre de Biotechnologie de Borj Cedria (CBBC), PB 901, 2050, Hammam Lif, Tunisie
Laboratoire des Légumineuses, Centre de Biotechnologie de Borj Cedria (CBBC), PB 901, 2050, Hammam Lif, Tunisie
Laboratoire des Légumineuses, Centre de Biotechnologie de Borj Cedria (CBBC), PB 901, 2050, Hammam Lif, Tunisie
Laboratoire des Légumineuses, Centre de Biotechnologie de Borj Cedria (CBBC), PB 901, 2050, Hammam Lif, Tunisie
Laboratoire des Grandes Cultures, Institut National de la Recherche Agronomique de Tunisie (INRAT), Université de Carthage, Rue Hédi Karray, 2080 Ariana, Tunisie
Laboratoire des Légumineuses, Centre de Biotechnologie de Borj Cedria (CBBC), PB 901, 2050, Hammam Lif, Tunisie
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