In order to achieve a high yield and quality of apple fruit, more effective ways of fertilization are required in the modern, high density apple orchards. The objective of this research was to determine the efficiency (partial nutrient balance, PNB) of different methods (foliar and fertrigation) and forms (chelates and salts) of microelements application in relation to the levels of N fertilization in apple orchard cultivar (‘Golden Delicious’). The combined effects of these fertilizers on the number of apple fruits per tree and on the yield per tree were also studied. Foliar application of Mn, Zn and Fe had significantly higher partial nutrient balance values compared to the soil application in both years of the experiment. However, most of the PNB values were below 10% indicating relatively low efficiency of the applied fertilizers with microelements.


foliar; fertigation; micronutrients; nitrogen; partial nutrient balance

Borg, S., Brinch-Pedersen, H., Tauris, B., Holm, P.B. (2009). Iron transport, deposition and bioavailability in the wheat and barley grain. Plant Soil, 325, 15–24, doi.org/10.1007/s11104-009-0046-6
Borowski, E., Michalek, S. (2011). The effect of foliar fertilization of French bean with iron salts and urea on some physiological processes in plants relative to iron uptake and translocation in leaves. Acta Sci. Pol. HortorumCultus., 10(2), 183–193.
Cakmak, I., Pfeiffer, W.H., McClafferty, B. (2010). Biofortification of durum wheat with zinc and iron. Cereal Chem., 87, 10–20, doi.org/10.1094/CCHEM-87-1-0010
Chaplin, H.M., Martin, L.W. (2008). The effect of nitrogen and boron fertilizer applications on leaf levels, yield and fruit size of the red raspberry. Commun. Soil Sci. Plant Anal., 11(6), 547–556, doi.org/10.1080/00103628009367062
Dimkpa, C.O., Bindraban, P.S. (2016). Fortification of micronutrients for efficient agronomic production: a review. Agron. Sustain. Dev., 36 (1), 7, doi.org/10.1007/s13593-015-0346-6
Doolette, C.L., Read, T.L., Li, C., Scheckel, K.G., Donner, E., Kopittke, P.M., Schjoerring, J.K., Lombi, E. (2018). Foliar application of zinc sulphate and zinc EDTA to wheat leaves: differences in mobility, distribution, and speciation. J. Exp. Bot., 69(18), 4469–4481. DOI: 10.1093/jxb/ery236
Fixen, P., Brentrup, F., Bruulsema, T., Garcia, F., Norton, R., Zingore, S. (2015). Nutrient/fertilizer use efficiency: measurement, current situation and trends. In: Managing water and fertilizer for sustainable agricultural intensification, Drechsel, P., Heffer, P., Magen, H., Mikkelsen, R., Wichelns, D. (eds). 1st ed. IFA, IWMI, IPNI, IPI, Paris, 8–37.
Hanson, E. (1996). Fertilizing fruit crops. Ext. Bull., E-852, 1–20.
Hoying, S., Fargione, M., Iungerman, K. (2004). Diagnosing apple tree nutritional status: leaf analysis interpretation and deficiency symptoms. Fruit Q., 12(1), 16–19.
ISO 14870:2001. Soil quality – Extraction of trace elements by buffered DTPA solution.
ISO 14235:2005. Soil quality – Determination of organic carbon by sulfochromic oxidation.
Jivan, C., Sala, F. (2014). Relationship between tree nutritional status and apple quality. Hortic. Sci., 41, 1–9.
Khoshgoftarmanesh, A.H., Schulin, R., Chaney, R.L., Daneshbakhsh, B., Afyuni, M. (2010). Micronutrient-efficient genotypes for crop yield and nutritional quality in sustainable agriculture. A review. Agron. Sustain. Dev. 30, 83–107. DOI: 10.1016/j.jplph.2013.08.008.
Lindsay, W.L., Norvell, W.A. (1978). Development of a DTPA soil test for zinc, iron, manganese and copper. Soil Sci. Soc. Am. J., 42, 421–428. DOI:10.2136/sssaj1978.03615995004200030009x
Marschner, H. (1988). Mechanisms of manganese acquisition by roots from soils. In: Manganese in soils and plants, Graham, R.D., Hannam, R.J., Uren, N.C. (eds.). Springer, Dordrecht.
Mengel, K. (1994). Iron availability in plant tissues-iron chlorosis on calcareous soil. Plant Soil., 165, 275–283, doi.org/10.1007/BF00008070
Milic, B., Cabilovski, R., Keserovic, Z., Manojlovic, M., Magazin, N., Doric, M. (2012). Nitrogen fertilization and chemical thinning with 6-benzyladenine affect fruit set and quality of golden delicious apples. Scientia Horticulturae, 140, 81–86. DOI: 10.1016/j.scienta.2012.03.029
Modaihsh, A.S. (1997). Foliar application of chelated and non-chelated metals for supplying micronutrients to wheat grown on calcareous soil. Exp. Agric., 33(2), 237–245. DOI: 10.1017/S001447979700001X
Morgan, B., Lahav, O. (2007). The effect of pH on the kinetics of spontaneous Fe (II) oxidation by O2 in aqueous solution – basic principles and a simple heuristic description. Chemosphere, 68(11), 2080–2084. DOI: 10.1016/j.chemosphere.2007.02.015
Neilsen, D., Neilsen, G.H. (2002). Efficient use of nitrogen and water in high-density apple orchards. Hort. Technol., 12, 19–25.
Neilsen, G.H., Hoyt, P. B. (1990). A comparison of methods to raise zinc concentration of apple leaves. Can.
J. Plant Sci., 70, 599–603. DOI: 10.4141/cjps90-075
Palmer, C.M., Guerinot, M.L. (2009). Facing the challenges of Cu, Fe and Zn homeostasis in plants. Nature Chem. Biol., 5, 333–340. DOI: 10.1038/nchembio.166.
Papadakis, I.E., Protopapadakis, E., Therios, I.N., Tsirakoglou, V. (2005). Foliar treatment of Mn deficient ‘Washington Navel’ orange trees with two Mn sources. Sci. Hortic., 106, 70–75, doi.org/10.1016/j.scienta.2005.02.015
Patel, N.M., Sadaria, S.G., Kaneria, B.B., Khanpara, V.D. (1995). Effect of nitrogen, potassium, and zinc on growth and yield of wheat (Triticum aestivum). Ind. J. Agron., 40(2), 290–92.
Porter, G.S., Bajita-Locke, J.B., Hue, N.V., Strand, D. (2004). Manganese solubility and phytotoxicity affected by soil moisture, oxygen levels, and green manure additions. Commun. Soil Sci. Plant Anal., 35(1–2), 99–116, doi.org/10.1081/CSS-120027637
Post-Beittenmiller, D. (1996). Biochemistry and molecular biology of wax production in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol., 47, 405–430, doi.org/10.1146/annurev.arplant.47.1.405
Raese, J.T., Drake, R.S., Curry, A.E., 2007. Nitrogen fertilizer influences fruit quality, soil nutrients and cover crops, leaf color and nitrogen content, biennial bearing and cold hardiness of ‘Golden Delicious’. J. Plant Nutr., 30, 1585–1604. doi.org/10.1080/01904160701615483
Shivay, Y., Prasad, R., Singh, M.P. (2014). Genetic variability for zinc use efficiency in chickpea as influenced by zinc fertilization. Int. J. Bio-res. Stress Manag., 5, 31–36.
Snyder, C.S., Bruulsema, T.W. (2007). Nutrient use efficiency and effectiveness in North America. Indices of agronomic and environmental benefits. International Plant Nutrition Institute.
Suzuki, M., Tsukamato, T., Inoue, H., Watanabe, S., Matsuhashi, S., Takahashi, M., Nakanishi, H., Mori, S., Nishizawa, N.K. (2008). Deoxymugineic acid increases Zn translocation in Zn deficienct rice plants. Plant Mol. Biol., 66, 609–617, doi: 10.1007/s11103-008-9292-x
Thalheimer, M., Paoli, N. (2002). Foliar absorption of Mn and Mg: effects of product formulation, period of application and mutual interaction of apple. Acta Hortic., 54, 157–164, doi: 10.17660/ActaHortic.2002.594.15
Wargo, M.J., Merwin, A.I., Watkins, B.C. (2003). Fruit size, yield and market value of ‘Gold Rush’ apple are affected by amount, timing and method of nitrogen fertilization. Hort. Technol., 13, 153–161.
Waters, B.M., Chu, H.H, DiDonato, R.J., Roberts, L.A., Eisley, R.B., Lahner, B., Salt, D.E., Walker, E.L. (2006). Mutations in Arabidopsis yellow stripe-like1 and yellow stripe-like3 reveal their roles in metal ion homeostasis and loading of metal ions in seeds. Plant Physiol., 141, 1446-1458. DOI: 10.1104/pp.106.082586

Published : 2019-10-30

Brayek, A., Čabilovski, R., Petković, K., Magazin, N., Čakmak, D., & Manojlovic, M. (2019). EFFICIENCY OF DIFFERENT METHODS AND FORMS OF MICROELEMENTS APPLICATION IN FUNCTION OF N FERTILIZER IN APPLE TREES. Acta Scientiarum Polonorum Hortorum Cultus, 18(5), 201-211. https://doi.org/10.24326/asphc.2019.5.20

Aboubaker H. Brayek 
Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21 000 Novi Sad, Serbia  Serbia
Ranko R. Čabilovski  ranko@polj.uns.ac.rs
Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21 000 Novi Sad, Serbia  Serbia
Klara M. Petković 
Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21 000 Novi Sad, Serbia  Serbia
Nenad P. Magazin 
Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21 000 Novi Sad, Serbia  South Africa
Dragan B. Čakmak 
Institute for Biological Research, University of Belgrade, Bulevar Despota Stefana 142, 11 060 Belgrade, Serbia  Serbia
Maja S. Manojlovic 
Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovića 8, 21 000 Novi Sad, Serbia  Serbia


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