ASCOCHYTA BLIGHT (Ascochyta syringae) OF LILAC (Syringa vulgaris L.)
Tomasz Kosiada
Poznań University of Life SciencesAbstract
Lilac (Syringa vulgaris L.) is a popular ornamental woody plant grown for its very decorative flowers and large, dark-green leaves. The leaves remain on the shrubs for a long time. The fungus, Ascochyta syringae, is a pathogen which deteriorates the decora-tive value of the leaves. It causes brown irregular spots on leaves. In this study, 20 fungal isolates were tested in terms of their pathogenicity towards the leaves of S. vulgaris, and mycelium growth rate, while genetic variability was determined by RAPD-PCR. It was found that some isolates do not cause the formation of brown spots on leaves. Isolates dif-fered considerably in terms of mycelium growth rate, ranging from 0.5 mm day-1 (B96 at 30°C) to 8.8 mm day-1 (B92a at 25°C). A positive dependence between mycelium growth and the capacity to cause leaf spots was observed. No close dependence was found be-tween the genetic variability of isolates and the other examined traits of the isolates.
Keywords:
disease, RAPD, genetic variabilityReferences
Bayazit, S., Imrak, B., Küden, A., Kemal Güngör, M. (2011). RAPD analysis of genetic related-ness among selected quince (Cydonia oblonga Mill.) accessions from different parts of Tur-key. Hort. Sci. (Prague), 38(4), 134–141.
Baturo-Ciesniewska, A. (2011). Genetic variability and pathogenicity among Polish isolates of Bipolaris sorokiniana (Cochliobolus sativus) derived from spring barley (Hordeum vul-gare L.). J. Plant Pathol., 93(2), 291–302.
Baturo-Ciesniewska, A., Grabowski, A., Panka, D. (2012). Diversity in the Polish isolates of Drechslera teres in spring barley as determined through morphological features, mating types, reaction to control agents and RAPD markers. J. Plant Pathol., 94(2), 339–351.
Canfield, M.L., Baca, S., Moore, L.W. (1986). Isolation of Pseudomonas syringae from 40 cultivars of diseased woody plants with tip dieback in Pacific Northwest nurseries. Plant Dis., 70(7), 647–650.
Canfield, M.L., Bateham, L.M., Moore, L.W. (1997). Resistance of Syringa vulgaris cultivars to Pseudomonas syringae infection. In: Rudolph, K., Burr, T.J., Mansfield, J., Stead, D.E., Vivi-an, A., von Kietzell, J. (eds). Pseudomonas Syringae Pathovars and Related Pathogens, Vol. 9, Developments in Plant Pathology. Springer Sci. & Business Media, 553–558.
Doyle, J.J. (1991). DNA protocols for plants. In: Molecular techniques in taxonomy, Hewitt, G., Johnson, A.W.B., Young, J.P.W. (eds). Berlin, Springer-Verlag, 283–293.
Frazzon, A.P.G., Matsumara, A.T.S., van der Sand, S.T. (2002). Morphological characterization and genetic analysis of Drechslera teres isolates. Genet. Mol. Biol., 25, 235–241.
Gutman, F., Nerd, A., Mizrahi, Y., Bar-Zvi, D., Raveh, D. (1999). Application of random amplified polymorphic DNA markers for identification of marula genotypes. HortSci., 34, 1256–1258.
Ii, Y., Uragami, A., Uno, Y., Kanechi, M., Inagaki, N., (2012) RAPD-based analysis of differences between male and female genotypes of Asparagus officinalis L. Hortic Sci., 39, 33–37.
Kosiada, T. (2012). In vitro growth of some species of Ascochyta Lib. Cent. Eur. J. Biol., 7(6), 1076–1083.
Machowicz-Stefaniak, Z., Zalewska, E., Król, E., Kowalik, B. (2012). Growth and development of Phomopsis diachenii Sacc. in different culture conditions. Acta Sci. Pol. Hortorum Cultus, 11(6), 69–80.
Melnik, V.A., Braun, U., Hagedor, G. (2000). Key to the fungi of the genus Ascochyta Lib. (Coe-lomycetes). Parey Buchverlag Berlin.
Sałata, B. (2002). Polskie gatunki grzybów mitosporowych z rodzaju Ascochyta. Wyd. UMCS, Lublin, pp. 121.
Punithalingam, E. (1979). Graminicolous Ascochyta species. Mycol. Pap. 142, CMI, Kew, U.K., pp. 214.
Weber, Z., Irzykowska, L., Bocianowski, J. (2005). Analysis of mycelial growth rates and RAPD-PCR profiles in a population of Gaeumannomyces graminis var. tritici originating from wheat plants grown from fungicide-treated seed. J. Phytopathol., 153, 318–324.
Poznań University of Life Sciences
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