Skip to main navigation menu Skip to main content Skip to site footer

Vol. 16 No. 3 (2017)

Articles

ASSESSMENT OF GENETIC DIVERSITY BETWEEN Vaccinium corymbosum L. CULTIVARS USING RAPD AND ISSR MARKERS

Submitted: October 19, 2020
Published: 2017-06-30

Abstract

In the present study genetic diversity between 19 blueberry cultivars was evaluated using Randomly Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) markers. In total, nine selected RAPD primers produced 89% polymorphic fragments, whereas eleven ISSR primers – 82%. Resolving power value of the RAPD primers ranged from 2.40 to 7.19, whilst ISSR from 1.90 to 5.78. The similarity coefficients estimated on the basis of the two types of marker systems were very similar, on average amounting to 0.58 for RAPD and 0.60 for ISSR analysis. Cluster analysis based on RAPD markers showed that the 19 accessions can be classified into 6 groups. Taking into account corresponding levels of average genetic similarity (0.59) it is possible to identify three main clusters based on ISSR analysis. The RAPD as well as ISSR markers revealed the existence of genetic differentiation between accessions, which can be exploited in hybridization programs of this species.

References

Albert, T., Raspe, O., Jacquemart, A.L. (2003). Clonal structure in Vaccinium myrtillus L. revealed by RAPD and AFLP markers. Int. J. Plant Sci., 164, 649–655.
Albert, T., Raspe, O., Jacquemart, A.L. (2005). Diversity and spatial structure of clones in Vaccinium uliginosum populations. Can. J. Bot., 83, 211–218.
An, D., Bykova, N.V., Debnath, S.C. (2015). EST-PCR, EST-SSR and ISSR markers to identify a set of wild cranberries and evaluate their relationship. Can. J. Plant Sci., 95, 1155–1165.
Anderson, M.J., Willis, T.J. (2003). Canonical analysis of principal coordinates: a useful method of constrained ordination for ecology. Ecology, 84, 511–525.
Bjedov, I., Obratov-Petković, D., Mišić, D., Šiler, B., Aleksic, J.M. (2015). Genetic patterns in range-edge populations of Vaccinium species from the central Balkans: implications on conservation prospects and sustainable usage. Silva Fenn., 49(4), https://doi.org/10.14214/sf.1283.
Boches, P., Bassil, N.V., Rowland, L. (2006). Genetic diversity in the highbush blueberry evaluated with microsatellite markers. J. Am. Soc. Hort. Sci., 131, 674–686.
Burgher, K.L., Jamieson, A.R., Lu, X. (2002). Genetic relationship among lowbush blueberry genotypes as determined by Random Amplified Polymorphic DNA
analysis. J. Am. Soc. Hortic. Sci., 127, 98–103.
Carvalho, M., Matos, M., Carnide, V. (2014). Fingerprinting of Vaccinium corymbosum cultivars using DNA of fruits. Hort. Sci. (Prague), 41, 175–184.
COBORU (2015). Research Centre for Cultivar Testing, http://www.coboru.pl (11.12.2015).
Debnath, S.C. (2005). Differentiation of Vaccinium cultivars and wild clones using RAPD markers. J. Plant Biochem. Biotech., 14, 173–177.
Debnath, S.C. (2007). Inter simple sequence repeat (ISSR) to assess genetic diversity in a collection of wild lignonberry (Vaccinium vitis-idaea L.) clones. Can. J. Plant Sci., 87, 337–344.
Debnath, S.C. (2009). Development of ISSR markers for genetic diversity studies in Vaccinium angustifolium. Nordic J. Bot., 27, 141–148.
Debnath, S.C. (2014a). Molecular markers in biodiversity of Vaccinium and Rubus wild germplasm. Acta Hortic., 1017, 85–92.
Debnath, S.C. (2014b). Structured diversity using ESTPCR and EST-SSR markers in a set of wild blueberry clones and cultivars. Bioch. Sys. Ecol., 54, 337–347.
Debnath, S.C., Khanizadeh, S., Jamieson, A.R., Kempler, C. (2008). Inter Simple Sequence Repeat (ISSR) markers to assess genetic diversity and relatedness within strawberry genotypes. Can. J. Plant Sci., 88, 313–322.
Degani, C., Rowland, L.J., Saunders, J.A., Ogden, S.C., Golan-Goldhirsh, E.L., Galleta, G.J. (2001). A comparison of genetic relationship measures in strawberry (Fragaria × ananassa Duch.) based on AFLPs, RAPDs, and pedigree data. Euphytica, 117, 1–12.
Doyle, J.J., Doyle, J.L. (1987). A rapid DNA isolation procedure from small quantities of fresh leaf tissue. Phytochem. Bull., 19, 11–15.
Ehlenfeldt, M.K., Vorsa, N., Draper, A.D. (2000). ‘Chanticleer’ highbush blueberry. HortScience, 35, 780–782.
Garriga, M., Parra, P.A., Caligari, P.D.S., Retamales, J.B., Carrasco, B.A., Lobos, G.A., Garcia-Gonzales, R. (2013). Application of inter-simple sequence repeats relative to simple sequence repeats a molecular marker system for indexing blueberry cultivars. Can. J. Plant Sci., 93, 913–921.
Georgiev, D., Georgieva, M., Dinkova, H. (2013). Growth and development of blueberry `variety ‘Brigitta Blue’ in the region of Troyan. Acta Hort., 981, 147–150.
Giongo, I., Vendramin, E., Quarta, R., Dettori, M.T., Verde, I., Bergamini, A. (2006). Use of RAPD analysis to characterize Vaccinium accessions. Acta Hort., 715, 153–156.
Haghighi, K., Hancock, J.F. (1992). DNA restriction fragment length variability in genomes of high-bush blueberry. HortScience, 27, 44–47.
Hammer, Ø., Harper, D.A.T., Ryan, P.D. (2001). Past: paleontological statistics software package for education and data analysis. Palaeontol. Electron., 4, 1–9.
Kaczmarska, E., Gawroński, J., Dyduch-Siemińska, M., Najda, A., Marecki, W., Żebrowska, J. (2015). Genetic diversity and chemical characterization of selected Polish and Russian cultivars and clones of blue honeysuckle (Lonicera caerulea). Turk. J. Agric. For., 39, 349–402.
Kenkel, N.C. (2006). On selecting an appropriate multivariate analysis. Can. J. Plant Sci., 86, 663–676.
Kizhakkayil, J., Sasikumar, B. (2010). Genetic diversity analysis of ginger (Zingiber officinale Rosc.) germplasm based on RAPD and ISSR markers. Sci. Hortic., 125, 73–76.
Koziński, B., (2012). The evaluation of growth and fruit productivity of six high-bush blueberry cultivars propagated by two vegetative methods. In: Books of Abstract
– 10th International Symposium of Vaccinium and other Superfruits, 17–21 June 2012, van Kooten, O., Brouns, F. (eds). Maastricht.
Kraciński, P., 2014. Harvesrting and disposal of the production of strawberries, raspberries and currants in Poland. Rocz. Nauk. Ekonom. Rol. Rozw. Obsz. Wiej., 101, 132–140.
Mańkowski, D.R., Laudański, Z., Janaszek, M. (2011). The application of chosen similarity measures for binary data in multivariate analysis in molecular experiments.
Biul. Inst. Hod. Aklim. Rośl., 262, 155–172.
Mantel, N. (1967). The detection of disease clustering and a generalized regression approach. Cancer Res., 27, 209–220.
Mirek, Z., Pięknoś-Mirkowa, H., Zając, A., Zając, M. (2002). Flowering plants and pteridophytes of Poland. A checklist. W. Szafer Institute of Botany, Polish Academy of Sciences, Cracow.
Nei, M., Li, W.H. (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci., 76, 5269–5273.
Oraguzie, N.C., Gardiner, S.E., Basset, H.C.M., Stefani, M., Ball, R.D., Bus, V.G.M., White, A.G. (2001). Genetic diversity and relationship in Malus sp. germplasm collections as determined by randomly amplified polymorphic DNA. J. Am. Soc. Hortic. Sci., 126, 318–328.
Peakall, R., Smouse, P.E. (2012). GenAlEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics, 28, 2537–2539.
Pliszka, K. (2004). Highbush blueberry ‘Bonifacy’. Szkółkarstwo, 3, 65.
Pluta, S., Żurawicz, E. (2014). The high-bush blueberry (Vaccinium corymbosum L.) breeding programme in Poland. Acta Hort., 1017, 177–180.
Prevost, A., Wilkinson, M.J. (1999). A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor. Appl. Genet., 98, 107–112.
Prodorutti, D., Pertot, I., Giongo, L., Gessler, C. (2007). Highbush blueberry: cultivation, protection breeding and biotechnology. Eur. J. Plant Sci. Biotech., 1, 44–56.
Reddy, P.M., Sarla, N., Siddiq, E.A. (2002). Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica, 128, 9–17.
Rejman, A., Pliszka, K. (1991). Borówka wysoka. PWRiL, Warszawa.
Rowland, L.J., Mehra, S., Dhanaraj, A.L., Ogden, E.L., Slovin, J.P., Ehlenfeld, M.K. (2003). Development of EST-PCR markers for DNA fingerprinting and genetic relationship studies in blueberry (Vaccinium, section Cyanococcus). J. Amer. Soc. Hort. Sci., 128, 682–690.
Shigyou, M., Gushiken, A., Katsuragawa, A., Hiji, M., Yoshikoa, K., Kage, T., Kunitake, H., Komatsu, H. (2014). Evaluation of F1 progenies derived from intersectional
crossing of Vaccinium uliginosum L., a wild species native to Japan, and highbush blueberry ‘Bluecrop’ (V. corymbosum L.). Hort. Res. (Japan), 13, 97–106.
Smolarz, K., Pluta, S. (2012). History of the commercial cultivation of the high-bush blueberry in Poland. In: Books of Abstract – 10th International Symposium of Vaccinium and other Superfruits, 17–21 June 2012, van Kooten, O., Brouns, F. (eds). Maastricht, The Netherlands.
Smolarz, K., Pluta, S. (2014). Cultivation of the high-bush blueberry in Poland. Acta Hortic., 1017, 199–204.
Wach, D., Gawroński, J., Dyduch-Siemińska, M., Kaczmarska, E., Błażewicz-Woźniak, M. (2016). Phenotypic and genotypic variability of cultivars of highbush blueberry (Vaccinium corymbosum L.) grown in the Lublin region. Acta Sci. Pol. Hortorum Cultus, 15, 305–319.
Wang, Z., Weber, J.L., Zhong, G., Tanksley, S.D. (1994). Survey of plant short tandem repeats. Theor. Appl. Genet., 88, 1–6.
Yakimowski, S.B., Eckert, C.G. (2008). Populations do not become less genetically diverse or more differentiated towards the northern limit of the geographical range in
clonal Vaccinium stamineum (Ericaceae). New Phytol., 180, 534–544.
Yu, H., Gu, Y., He, S., Jiang, Y. (2009). Identification of blueberry cultivars and evaluation of their genetic relationship by ISSR technique. J. Jilin Agric. Univ., 31, 512–515.
Zheng, S., Zhang, L., Xie, L., Zhang, X., Li, T. (2014). DNA fingerprinting by ISSR markers for 17. blueberries. Fujian J. Agr. Sci., 29, 1198–1201.
Zoratti, L., Palmieri, L., Jaakola, L., Häggman, H. (2015). Genetic diversity and population structure of an important wild berry crop. AoB Plants. 7, plv117,
https://doi.org/10.1093/aobpla/plv117.

Downloads

Download data is not yet available.

Most read articles by the same author(s)

1 2 > >> 

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.