EVALUATION OF GRAPEVINE RESISTANCE TO DOWNY AND POWDERY MILDEW IN ‘REGENT’ × ‘BOĞAZKERE’ HYBRID POPULATION SEGREGATING FOR RESISTANCE GENES
Mina ShidfarAnkara University, Horticulture Department, 06110 Ankara, Turkey
Murat AkkurtAnkara University, Horticulture Department, 06110 Ankara, Turkey
Arif AtakAtaturk Horticulture Central Research Institute, 77102 Yalova, Turkey
Ali ErgülAnkara University, Biotechnology Institute, 06110 Ankara, Turkey
Gökhan SöylemezoğluAnkara University, Horticulture Department, 06110 Ankara, Turkey
Downy (Plasmopara viticola) and powdery mildew (Erysipha necator) is known as one of the most mischievous diseases for viticulture in Turkey as well as in the world. Therefore breeding studies play an important role for development of new cultivars resistant against fungal diseases. The aim of this research was to develop new F1 population and evaluate the resistance of hybrids to powdery and downy mildew via marker assisted selection (MAS). Resistant ‘Regent’ and susceptible ‘Boğazkere’ varieties were used to obtain hybrid population. A total of 6 markers belonging to 3 genomic regions were used for DNA based selection. Four SSR (UDV15, VMCNG2f12, VMC7F2 and UDV305) and two SCAR (ScORNA7-760 and ScORN3-R) markers which were developed to the resistance loci of Rpv3 and Ren3 were used for DNA based selection. The results were evaluated together with powdery mildew inoculation observations. When inoculation observations and MAS were evaluated, genotypes resistant, tolerant and susceptible to powdery and downy mildew were identified. Especially 16 genotypes identified as resistant to powdery mildew, can be used in future breeding programs.
Keywords:breeding, marker-assisted selection, grapevine, ‘Boğazkere’, ‘Regent’, powdery mildew
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