Skip to main navigation menu Skip to main content Skip to site footer

Vol. 18 No. 1 (2019)

Articles

EVALUATION OF GRAPEVINE RESISTANCE TO DOWNY AND POWDERY MILDEW IN ‘REGENT’ × ‘BOĞAZKERE’ HYBRID POPULATION SEGREGATING FOR RESISTANCE GENES

DOI: https://doi.org/10.24326/asphc.2019.1.18
Submitted: February 22, 2019
Published: 2019-02-22

Abstract

Downy (Plasmopara viticola) and powdery mildew (Erysipha necator) is known as one of the most mischievous diseases for viticulture in Turkey as well as in the world. Therefore breeding studies play an important role for development of new cultivars resistant against fungal diseases. The aim of this research was to develop new F1 population and evaluate the resistance of hybrids to powdery and downy mildew via marker assisted selection (MAS). Resistant ‘Regent’ and susceptible ‘Boğazkere’ varieties were used to obtain hybrid population. A total of 6 markers belonging to 3 genomic regions were used for DNA based selection. Four SSR (UDV15, VMCNG2f12, VMC7F2 and UDV305) and two SCAR (ScORNA7-760 and ScORN3-R) markers which were developed to the resistance loci of Rpv3 and Ren3 were used for DNA based selection. The results were evaluated together with powdery mildew inoculation observations. When inoculation observations and MAS were evaluated, genotypes resistant, tolerant and susceptible to powdery and downy mildew were identified. Especially 16 genotypes identified as resistant to powdery mildew, can be used in future breeding programs.

References

  1. Akkurt, M., Welter, L., Töpfer, R., Zyprian, E. (2007). Development of SCAR markers linked to downy mildew (Plasmopora viticola) resistance in grapevine (Vitis vinifera L.). Mol. Breed., 19, 103–111.
  2. Alleweldt, G., Dettweiler-Munch, E. (1992). The genetic resources of Vitis. Genetic and geographic origin of grape cultivars, their prime names and synonyms, 3th ed. Institute for Grapevine Breeding Geilweilerhof, Siebeldingen, Germany.
  3. Bellin, D., Peressotti, E., Merdinoglu, D., Wiedemann-Merdinoglu, S., Adam-Blondon, A.F., Cipriani, G., Morgante, M., Testolin, R., Di Gaspero, G. (2009). Resistance to Plasmopara viticola in grapevine ‘Bianca’ is controlled by a major dominant gene causing localised necrosis at the infection site. Theor. Appl. Genet., 120, 163–176
  4. Bertrand, C., Collar, Y., Mackill, D.J. (2007). Marker-assisted selection: an approach for precision plant breeding in the twenty-first century. Philos. Trans. R. Soc. B., 363, 557–572.
  5. Blanc, S., Wiedemann-Merdinoglu, S., Dumas, V., Mestre, P., Merdinoglu, D. (2012). A reference genetic map of Muscadinia rotundifolia and identification of Ren5, a new major locus for resistance to grapevine powdery mildew. Theor. Appl. Genet., 125, 1663–1675.
  6. Bouquet, A., Pauquet, J., Adam-Blondon, A.F., Torregrosa, L., Merdinoglu, D., Wiedemann-Merdinoglu, S. (2000). Vers l’obtention de variétés de vigne résistantes à l’oidium et au mildiou par les méthodes conventionnelles et biotechnologiques. Prog. Agric. Vitic., 117, 383–389.
  7. Cadle-Davidson, L., Mahanil, S., Gadoury, D.M., Kozma, P., Reisch, B.I. (2011). Natural infection of Run1-positive vines by native genotypes of Erysiphe necator. Vitis, 50(4), 173–175.
  8. Calonnec, A., Cartolaro, P., Poupot, C., Dubourdieu, D., Darriet, P. (2004). Effects of Uncinula necator on the yield and quality of grapes (Vitis vinifera) and wine. Plant Pathol., 53, 434–445.
  9. Di Gaspero, G., Cattonaro, F. (2010). Application of genomics to grapevine improvement. Aust. J. Grape Wine Res., 16, 122–130. DOI: 10.1111/j.1755-0238.2009.00072.x.
  10. Di Gaspero, G., Copetti, D., Coleman, C., Castellarin, S.D., Eibach, R., Kozma, P., Lacombe, T., Gambetta, G., Zvyagin, A., Cindric, P., Kovács, L., Morgante, M., Testolin, R. (2012). Selective sweep at the Rpv3 locus during grapevine breeding for downy mildew resistance. Theor. Appl. Genet., 124, 277–286.
  11. Eibach, R., Töpfer, R. (2003). Success in resistance breeding: “Regent” and its steps into the market. Acta Hortic., 603, 687–691. DOI: 10.17660/ActaHortic.2003.603.95.
  12. Eibach, R., Zyprian, E., Welter, L., Töpfer, R. (2007). The use of molecular markers for pyramiding resistance genes in grapevine breeding. Vitis, 46(2), 120–124.
  13. Fischer, B.M., Slakhutdinov, I., Akkurt, M., Eibach, R., Edwards, K.J., Töpfer, R., Zyprian, E.M. (2004). Quantitative trait locus analysis of fungal disease resistance factors on a molecular map of grapevine. Theor. Appl. Genet., 108, 501–515.
  14. Furuya, S., Mochizuki, M., Saito, S., Kobayashi, H., Takayanagi, T., Suzuki, S. (2010). Monitoring of QoI fungicide resistance in Plasmopara viticola populations in Japan. Pest Manag. Sci., 66, 1268–1272.
  15. GENRES-081 (1997). Descriptors for grapevines (Vitis spp.). International Union for the Protection of New Varieties of Plants, Geneva, Switzerland/Office International de la Vigne et du Vin, Paris, France/International Plant Genetic Resources Institute, Rome, Italy.
  16. Guillaumie, S., Ilg, A., Réty, S., Brette, M., Trossat-Magnin, C., Decroocq, S., Léon, C., Keime, C., Ye, T., Baltenweck-Guyot, R., Claudel, P., Bordenave, L., Vanbrabant, S., Duchêne, E., Delrot, S., Darriet, P., Hugueney, P., Gomès, E. (2013). Genetic analysis of the biosynthesis of 2-methoxy-3-isobutylpyrazine, a major grape-derived aroma compound impacting wine quality. Plant Physiol., 162, 604–15.
  17. Kono, A., Sato, A., Reisch, B., Cadle-Davidson, L. (2015) Effect of detergent on the quantification of grapevine downy mildew sporangia from leaf discs. HortScience, 50, 656–660.
  18. Lefort, F., Lally, M., Thompson, D., Douglas, G.C. (1998). Morfolojical traits microsatellite fingerprinting and genetic relatedness of a stand of elite oaks (Q. robur L.) at Tullynally, Ireland. Silvae Genet. 47, 257–262.
  19. McGovern, P.E. (2003). Ancient wine: the search for the origins of viniculture. NJ Princeton University Press, Princeton.
  20. Riaz, S., Tenscher, A.C., Ramming, D.W., Walker, M.A. (2011). Using a limited mapping strategy to identify major QTLs for resistance to grapevine powdery mildew (Erysiphe necator) and their use in marker-assisted breeding. Theor. Appl. Genet. 122, 1059–1073
  21. Wan, Y., Schwaniniger, H., He, P., Wang, Y. (2007). Comparison of resistance to powdery mildew and downy mildew in Chinese wild grapes. Vitis, 46, 132–136.
  22. Welter, L.J., Göktürk-Baydar, N., Akkurt, M., Maul, E., Eibach, R., Töpfer, R., Zyprian, M.E. (2007). Genetic mapping and localization of quantitative trait loci affecting fungal disease resistance and leaf morphology in grapevine (Vitis vinifera L.). Mol. Breed., 20, 359–374
  23. Zini, E., Raffeiner, M., Di Gaspero, G., Eibach, R., Grando, M.S., Letschka, T. (2015). Applying a defined set of molecular markers to improve selection of resistant grapevine accessions. Acta Hortic., 1082, 73–78. DOI: 10.17660/ActaHortic.2015.1082.9

Downloads

Download data is not yet available.

Similar Articles

<< < 1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.