ANTIOXIDANT ACTIVITY AND CYTOTOXIC EFFECT OF CHILEAN Buddleja globosa (MATICO) AND Ribes magellanicum (ZARZAPARRILLA) FLOWER EXTRACTS
Pedro Zamorano-Aguilar
Graduate School, Faculty of Agricultural Science, Universidad Austral de Chile, Av. Carlos Ibañez del Campo S/N Valdivia, Chilehttps://orcid.org/0000-0003-0452-2239
Marcela Morales
Grupo Química de los Productos Naturales y los Alimentos, Facultad de Ciencias, Universidad Nacional de Colombia, Calle 59a N° 63-20 Medellín, ColombiaYudrum Rivillas
Grupo Biotecnología Animal, Facultad de Ciencias, Universidad Nacional de Colombia, Calle 59a N° 63-20 Medellín, ColombiaJuan López
Grupo Biotecnología Animal, Facultad de Ciencias, Universidad Nacional de Colombia, Calle 59a N° 63-20 Medellín, ColombiaBenjamín A. Rojano
Grupo Química de los Productos Naturales y los Alimentos, Facultad de Ciencias, Universidad Nacional de Colombia, Calle 59a N° 63-20 Medellín, Colombiahttps://orcid.org/0000-0003-3590-8046
Abstract
The native Chilean shrubs Buddleja globosa (matico) and Ribes magellanicum (zarzaparrilla) are used widely at a rural level, due to their medicinal properties. Nevertheless, little is known about their secondary metabolites and cytotoxic effect. The aim of this study was to evaluate the content of different compounds like catechin, epicatechin, p-coumaric acid and the antioxidant capacity by ABTS, ORAC, FRAP and DPPH methods. In addition, the cytotoxic activity of both extracts was evaluated against Chinese hamster ovary (CHO-K1) cell lines by MTT and neutral red assays. The results suggest that the most abundant constituent in Budleja globosa and Ribes magellanicum were catechin (682.43 mg/100 g DW) and epicatechin (3362.08 mg/100 g DW) respectively; while the ORAC methodology showed an elevated antioxidant activity for matico (134147.31 μmol Trolox Eq/100 g DW). On the other hand, both extracts at the assayed concentrations affect the membrane stability and cellular metabolic capacity of the CHO-K1 cell lines. These finding provide a direction for further researches, and suggest the matico and zarzaparrilla flower extracts as promising sources of antioxidants, and as research objects through the analyze of their metabolic behavior and antitumoral potential.
Keywords:
matico, zarzaparrilla, antioxidant capacity, cytotoxic activity, bioactive compounds, polyphenolsReferences
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Graduate School, Faculty of Agricultural Science, Universidad Austral de Chile, Av. Carlos Ibañez del Campo S/N Valdivia, Chile https://orcid.org/0000-0003-0452-2239
Grupo Química de los Productos Naturales y los Alimentos, Facultad de Ciencias, Universidad Nacional de Colombia, Calle 59a N° 63-20 Medellín, Colombia
Grupo Biotecnología Animal, Facultad de Ciencias, Universidad Nacional de Colombia, Calle 59a N° 63-20 Medellín, Colombia
Grupo Biotecnología Animal, Facultad de Ciencias, Universidad Nacional de Colombia, Calle 59a N° 63-20 Medellín, Colombia
Grupo Química de los Productos Naturales y los Alimentos, Facultad de Ciencias, Universidad Nacional de Colombia, Calle 59a N° 63-20 Medellín, Colombia https://orcid.org/0000-0003-3590-8046
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