MICROPROPAGATION OF WILD CHICORY (Cichorium intybus L. var. silvestre Bisch.) FROM LEAF EXPLANTS

Romuald Doliński

University of Life Sciences in Lublin

Anna Olek

University of Life Sciences in Lublin


Cichorium intybus is edible, medicinal and forage plant. The pharmaceutical raw materials were obtained from wild chicory (var. silvestre). Currently, farmers are increasingly assume plantations of wild chicory, and breeders are attempting to produce cultivars for medicinal purposes. In the modern breeding of chicory important feature is the ability to clonal propagation in vitro culture. The aim of our study was to assess capacity of natural population of wild chicory for plant regeneration from leaf explants. In the first was examined the effect of 16 combinations of various concentrations of IAA and 2iP on the regeneration of shoots from leaf explants (0.5 cm2). After that, 25 plants were propagated on the medium which was found as optimal. Then, their callus growth and shoots
regeneration capacities were compared. Later on, was examined the effect of various IAA concentrations on the rooting of shoots. The majority of the shoots was regenerated from callus but direct organogenesis was also observed (8%). Shoot regeneration was found to be the most efficient on MS medium containing 0.5 mg dm-3 IAA and 4 mg dm-3 2iP – 97% of the explants produced shoots, while the average number of shoots was 15.5. The amount of callus was found to be a highly heritable trait (h2 = 0.83). Lower the heritability coefficients were obtained for the number of shoots per explant (0.55) and the average shoot weight (0.40). The wild chicory shoots rooted easily. The number and weight of roots increased with the increasing concentration of IAA.


Cichorium intybus, in vitro propagation, organogenesis, heritability coefficients

Bais H.P., Ravishankar G.A., 2001. Cichorium intybus L. – cultivation, processing, utility, value addition and biotechnology, with an emphasis on current status and future prospects. J. Sci. Food Agric. 18, 467–484.
Bellettre A., Couillerot J.P., Vasseur J., 1999. Effect of glycerol on somatic embryogenesis in Cichorium leaves. Plant Cell. Rep. 19, 26–31.
Falconer D.S., 1989. Introduction to quantitative genetics. 3rd Ed. Longman Scientific and Technical, Harlow.
Genga A., Giansante L., Bernacchia G., Allavena A., 1994. Plant regeneration from Cichorium intybus L. leaf explants transformed by Agrobacterium tumefaciens. J. Genet. Breed. 48, 25–32.
Li G.D., Kemp P.D., 2005. Forage chicory (Cichorium intybus L.): A review of its agronomy and animal production. Adv. Agron. 88, 187–222.
Nandagopal S., Ranjitha Kumari B.D.R., 2006. Adenine sulphate induced high frequency shot organogenesis in callus and in vitro flowering of Cichorium intybus L. cv. Fokus – a potent medicinal plant. Acta Agricult. Slov. 87 (2), 415–425.
Pieron S., Belaizi M., Boxus P., 1993. Nodule culture, a possible morphogenetic pathway in Cichorium intybus L. propagation. Sci. Hort. 53, 1–11.
Previati A., Benelli C., Da Re F., De Carlo A., Vettori C., Lambardi M., 2005. In vitro propagation and conserwation of red chicory germplasm. The role of biotechnology. Villa Gualiano, Turin, Italy, 5–7 March, 207–208.
Rehman R.U., Israr M., Srivastava P.S., Bansal K.C., Abdin M.Z., 2003. In vitro regeneration of witloof chicory (Cichorium intybus L.) from leaf explants and accumulation of esculin. In Vitro Cell. Dev. Biol. – Plant 39, 142–146.
Rickett H.W., 1967. Wild flores of the United States. Parts 1–4. New York, McGraw-Hill Book Co.
Senderski M.E., 2009. Praktyczny poradnik o ziołach i ziołolecznictwie. Wyd. K.E. Liber, Warszawa, 102–103.
Tutin T.G., Burges N.A., Chater A.O., Edmondson J.R., Heywood V.H., Moore D.M., Valentine D.H., Walters S.M., Webb D.A., 2010. Flora Europea. Cambridge University Press. Vol. 4, 304–305.
Velayutham P., Ranjithakumari B.D., Baskaran P., 2006. An efficient in vitro plant regeneration system for Cichorium intybus L. – an important medicinal plant. J. Agric. Tech. 2(2), 287–298.
Vavilov N.L., 1951. The origin, variation, immunity and breeding of cultivated plants. Chron. Bot. 13, 1–366.
Yucesan B., Turker A.U., Gurel E., 2007. TDZ-induced high frequency plant regeneration through multiple shoot formation in witloof chicory (Cichorium intybus L.). Plant Cell Tiss. Organ Cult. 91, 243–250.


Romuald Doliński 
University of Life Sciences in Lublin
Anna Olek 
University of Life Sciences in Lublin



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