Skip to main navigation menu Skip to main content Skip to site footer
ASPHC Baner

Vol. 23 No. 2 (2024)

Articles

Regenerative potential and its variability in different topophysical zones of Kalanchoe daigremontiana leaves in in vitro culture conditions

DOI: https://doi.org/10.24326/asphc.2023.5280
Submitted: September 29, 2023
Published: 2024-04-30

Abstract

Plants of the genus Kalanchoe are the most frequently purchased potted plants because of their decorative qualities and easy cultivation. Modern commercial plants have unique shapes that are usually not genetically determined; hence, in vitro cultures are used to propagate such specimens. The efficiency of regeneration is significantly affected by the type of explant used when that explant was previously located on one of several parts of a complex plant organ. The study aimed to investigate the influence of the topophysical location of collected explants on the regeneration efficiency of adventitious shoots. Various regeneration pathways were observed in the in vitro micropropagation of Kalanchoe daigremontiana leaf fragments, i.e., direct and indirect organogenesis from the callus stage. The results show a dependent relationship between the location of the collected explant and its regenerative potential. The most remarkable regenerative capacity of K. daigremontiana was found in leaf blade fragments taken from the middle and proximal zones. In addition, the explants collected from the marginal zone of the leaf blade were notably more valuable than those from the part located near the midrib. The research results indicate that the growth and development of new seedlings propagated vegetatively with the in vitro method depends on their location on the mother plant.

References

  1. Bidabadi, S.S., Jain, S.M. (2020). Cellular, molecular, and physiological aspects of in vitro plant regeneration. Plants (Basel), 9(6), 702. https://doi.org/10.3390/plants9060702. PMID: 32492786; PMCID: PMC7356144. DOI: https://doi.org/10.3390/plants9060702
  2. Buyun, L., Ivannikov, R., Batsmanova, L., Taran, N., Kovalska, L., Gurnenko, I., Maryniuk, M. (2021). Structural adaptation and antioxidant response of Guarianthe bowringiana (O’Brien) Dressler & WE Higgins (Orchidaceae Juss.) seedlings during ex vitro acclimatization. Acta Agrobot., 74(1), 7422. https://doi.org/10.5586/aa.7422 DOI: https://doi.org/10.5586/aa.7422
  3. Cantrill, L.C., Overall, R.L., Goodwin, P.B. (2001). Changes in symplastic permeability during adventitious shoot regeneration in tobacco thin cell layers. Planta, 214, 206–214. https://doi.org/10.1007/s004250100620 DOI: https://doi.org/10.1007/s004250100620
  4. De Ruiter, H.A. (1996). Development of chrysanthemum cuttings: the influence of age and position of the axillary buds. Ann. Bot., 77(1), 99–104. DOI: https://doi.org/10.1006/anbo.1996.0012
  5. Dimitrova, N., Nacheva, L. (2021). An optimized micropropagation protocol by ex vitro rooting of pear rootstock OHF 333 (Pyrus communis L.). Acta Agrobot., 74(1), 744. https://doi.org/10.5586/aa.744 DOI: https://doi.org/10.5586/aa.744
  6. Frello, S., Venerus E., Serek M. (2002). Regeneration of various species of Crassulaceae, with special reference to Kalanchoe. J. Hortic. Sci. Biotechnol., 77(2), 204–208. https://doi.org/10.1080/14620316.2002.11511480 DOI: https://doi.org/10.1080/14620316.2002.11511480
  7. Garcês, H., Sinha, N. (2009). The ‘mother of thousands’(Kalanchoë daigremontiana): a plant model for asexual reproduction and CAM studies. Cold Spring Harb. Protoc., 10. https://doi.org/10.1101/pdb.emo133 DOI: https://doi.org/10.1101/pdb.emo133
  8. Izumikawa, Y., Nakamura, I., Mii, M. (2006). Interspecific hybridization between Kalanchoe blossfeldiana and several wild Kalanchoe species with ornamental value. Acta Hort., 743, pp. 59–65. https://doi.org/10.17660/ActaHortic.2007.743.7 DOI: https://doi.org/10.17660/ActaHortic.2007.743.7
  9. Kato, M. (1996). Somatic embryogenesis from immature leaves of in vitro grown tea shoots. Plant Cell Rep., 15, 920–923. https://doi.org/10.1007/BF00231588 DOI: https://doi.org/10.1007/BF00231588
  10. Kertrung, T., Junkasiraporn, S. (2018). In vitro propagation of Kalanchoe rhombopilosa (Crassulaceae). NU Int. J. Sci., 15(1), 37–48.
  11. Kuang, Y., Lu, C.H., Hsu, F.C. (2021). Restoring fer¬tility for novel interspecific hybrids between Kalanchoe garambiensis and K. nyikae using colchicine treatment. Plants, 10, 209, 16 pp. https://doi.org/10.3390/plants10020209 DOI: https://doi.org/10.3390/plants10020209
  12. Le Bris, M., Michaux-Ferrière, N., Jacob, Y., Poupet, A., Barthe, P., Guigonis, J.M., Le Page-Degivry, M.T. (1999). Regulation of bud dormancy by manipulation of ABA in isolated buds of Rosa hybrida cultured in vitro. Funct. Plant Biol., 26(3), 273–281. https://doi.org/10.1071/pp98133 DOI: https://doi.org/10.1071/PP98133
  13. Ludvová, A., Ostrolucká, M.G. (1998). Morphogenic processes in callus tissue cultures and de novo regeneration of plants in Actinidia chinensis Planch. Acta Soc. Bot. Pol., 67(3–4), 217–222. https://doi.org/10.5586/asbp.1998.025 DOI: https://doi.org/10.5586/asbp.1998.025
  14. Miler, N., Zalewska, M. (2014). Somaclonal variation of chrysanthemum propagated in vitro from different explants types. Acta Sci. Pol. Hortorum Cultus, 13(2), 69–82.
  15. Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Phys. Plant., 15(3), 473–497. DOI: https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  16. Pacholczak, A., Nowakowska, K. (2020). The effect of biostimulators and indole-3-butyric acid on rooting of stem cuttings of two ground cover roses. Acta Agrobot., 73(1), 7314. https://doi.org/10.5586/aa.7314 DOI: https://doi.org/10.5586/aa.7314
  17. Peer, K.R., Greenwood, M.S. (2001). Maturation, topophysis and other factors in relation to rooting in Larix. Tree Physiol., 21(4), 267–272. https://doi.org/10.1093/treephys/21.4.267 DOI: https://doi.org/10.1093/treephys/21.4.267
  18. Previati, A., Benelli, C., Da Re, F., Giannini, M. (2008). In vitro production of virus-free chrysanthemum stock plants for cut flowers. Propag. Ornam. Plant, 8, 167–169.
  19. Radomir, A.M., Stan, R., Pandelea, M.L., Vizitiu, D.E. (2022). In vitro multiplication of Mentha piperita L. and comparative evaluation of some biochemical compounds in plants regenerated by micropropagation and conventional method. Acta Sci. Pol. Hortorum Cultus, 21(4), 45–52. https://doi.org/10.24326/asphc.2022.4.5 DOI: https://doi.org/10.24326/asphc.2022.4.5
  20. Ruzin, S.E. (1999). Plant microtechnique and microscopy. Vol. 198. Oxford University Press, New York, p. 322.
  21. Sanikhani, M., Frello, S., Serek, M. (2006). TDZ induces shoot regeneration in various Kalanchoe blossfeldiana Poelln. cultivars in the absence of auxin. Plant Cell Tiss. Organ Cult., 85, 75–82. https://doi.org/10.1007/s11240-005-9050-6 DOI: https://doi.org/10.1007/s11240-005-9050-6
  22. Solís, J. J., Reyna, M., da Feria, M., Cardona, M. A., Rojas, D. (2013). In vitro propagation of Echeveria elegans, a species of the flora endangered Mexican. J. Environ. Sci. Engineer. B, 2(9B), 555–558.
  23. Smith, G.F. (2020). Plant smart. The big easy. Gardening with Kalanchoe. Veld Flora, 106(3), 40–43.
  24. Smith, G.F., Shtein, R. (2022). A review of horticulturally desirable characters in Kalanchoe (Crassulaceae subfam. Kalanchooideae): variable and deviating vegetative and reproductive morphologies useful in breeding programmes. Haseltonia, 28(1), 106–119. https://doi.org/10.2985/026.028.0110 DOI: https://doi.org/10.2985/026.028.0110
  25. Smith, R.H., Nightingale, A.E. (1979). In vitro propagation of Kalanchoe. HortScience, 14, 20. DOI: https://doi.org/10.21273/HORTSCI.14.1.20
  26. Su, Y.H., Liu, Y. B., Zhang, X.S. (2011). Auxin–cytokinin interaction regulates meristem development. Mol. Plant, 4(4), 616–625. https://doi.org/10.1093/mp/ssr007 DOI: https://doi.org/10.1093/mp/ssr007
  27. Schwaiger, G., Horn, W. (1987). Somaclonal variations in micropropagated Kalanchoe hybrids. Acta Hort., 226, 695–698. https://doi.org/10.17660/ActaHortic.1988.226.98 DOI: https://doi.org/10.17660/ActaHortic.1988.226.98
  28. Wendling, I., Brooks, P.R., Trueman, S.J. (2015). Topophysis in Corymbia torelliana× C. citriodora seedlings: adventitious rooting capacity, stem anatomy, and auxin and abscisic acid concentrations. New For., 46, 107–120. https://doi.org/10.1007/s11056-014-9451-7 DOI: https://doi.org/10.1007/s11056-014-9451-7
  29. Zalewska, M., Miler, N. (2010). Topophysis in adventitious shoots regeneration in vitro in chrysanthemum. Biotechnologia, 2(89), 89–95.

Downloads

Download data is not yet available.

Most read articles by the same author(s)

Similar Articles

1 2 3 4 5 6 7 8 9 10 > >> 

You may also start an advanced similarity search for this article.