In vitro POLLEN VIABILITY, GERMINATION AND POLLEN TUBE GROWTH IN SOME POMEGRANATE (Punica granatum L.) CULTIVARS FROM CROATIA AND BOSNIA AND HERZEGOVINA
Jelena GadžeUniversity of Zagreb, Croatia
Mira RadunićInstitute for Adriatic Crops and Karst Reclamation Split, Croatia
Ivana Vladimira PetricCroatian Centre for Agriculture, Zagreb, Croatia
Sezai ErcisliAtaturk University, Erzurum, Turkey
In this study in vitro pollen viability and germination in intact pollens and pollen tube growth in germinated pollens of five common pomegranate cultivars grown in
Croatia and Bosnia and Herzegovina was investigated first time. Pollen viability varied from 36.73% (cv. Konjski Zub) to 51.80% (cv. Barski) in fluorescent diacetat (FDA) test. The average pollen germination percentages were found as the lowest 6.83% in cv. Konjski Zub and the highest 42.51% in cv Glavas. Among the germination media in general 0.2 agar + 10% sucrose+5 ppm H3BO3 gave better results to obtain higher pollen germination for all cultivars. In germinated pollens of these five cultivars, significant differences were observed on pollen tube length. Average pollen tube lengths in germinated pollens of five cultivars were measured as minimum 302 μm in cv. Konjski Zub and as maximum 344 μm in cv. Ciparski. The results showed that there were obvious differences in pollen
germinability among cultivars growing under the same environmental conditions and these differences must be considered for orchard establishment to obtain higher yield.
Keywords:pomegranate, Punica granatum, cultivars, pollen germination, pollen tube growth
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