THE EFFECTIVENESS OF ANTAGONISTIC FUNGI AND THEIR POST-CULTURE LIQUIDS IN PEA (Pisum sativum L.) PROTECTION AGAINST DISEASES
Danuta Pięta
University of Life Sciences in LublinAlina Pastucha
University of Life Sciences in LublinAbstract
The aim of research was to determine the protective effect of the microbiological material Trichoderma harzianum G 220 and Gliocladium fimbriatum S 151 and their post-culture liquids against soil-borne pathogenic fungi. The use of biological control improved the emergences, healthiness of pea plants and their yielding. Despite using the microbiological material for seed dressing, pea plants were infected by Altenaria alternata, Ascochyta pisi, Botrytis cinerea, Fusarium culmorum, Fusarium oxysporum f. sp. pisi, Fusarium solani, Phoma exigua, Pythium irregulare, Rhizoctonia solani and Sclerotinia sclerotiorum.
Keywords:
pea, Trichoderma harzianum, Gliocladium fimbriatum, post-culture liquidsReferences
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Benhamou N., Chet I., 1996. Parasitism of sclerotia of Sclerotium rolfsii by Trichoderma harzianum: Ultrastructural and cytochemical aspects of the interaction. Phytopathol., 86, 405–416.
Berg G., Roskot N., Steidle A., Eberl L., Zock A., Smalla K., 2002. Plant-diseases phenotypic diversity of antagonistic rhizobacteria isolated from different Verticillium dahliae Kleb. Appl. Environ. Microbiol., 68, 3328–3338.
Dubay S. C., 2002. Bio-agent based integrated management of collar rot of french bean. Indian Phytopathol., 55, 2, 230–231.
Elad J., 2000. Trichoderma harzianum T 39 preparation for biocontrol of plant diseases – control of Botrytis cinerea, Sclerotinia sclerotiorum and Cladosporium fulvum. Biocontrol Sci. and Technology, 10, 4, 499–507.
El-Kafrawy A. A., 2002. Biological control of bean damping of caused by Rhizoctonia solani Egiptian J. Agric. Res., 80, 1, 57–70.
Filipowicz A., 1983. Grzyby zasiedlające więdnący groch siewny oraz podatność na nie różnych odmian tej rośliny. Zesz. Prob. Post. Nauk Rol. 275, 107–120.
Filipowicz A., 1993. Fungi colonizing and decaying plants of pea (Pisum sativum L.). Phytopath. Pol., (XVIII), 6, 73.
Gherbawy Y. A. M. H., Yaser M., 2003. Fungicides and some biological controller agents effect on the growth of Fusarium oxysporum causing paprika wilt. Rev. Plant Pathol., 83 (5), 585.
Gurha S. N., 2001. Effect of some Trichoderma spp. on the growth of different isolates of Fusarium oxysporum f. sp. ciceris, in vitro. Ann. Plant Protect. Sci., 9, 2, 332–334.
Hagedorn D. J., 1989. Compedium of pea diseases. The Amerian Phytopath. Soc., USA.
Harman G. E., Hayes C. K., Lorito M., Di Pietro A., Brodway R. M., Tronsmo A., 1993. Chitinolytic enzymes produced by Trichoderma harzianum, purification of chitobiosidase. Phytopathology, 83, 313–318.
Haran S., Schickler H., Oppenheim A., Chet I., 1995. New components of the chitinolytic system of Trichoderma harzianum. Mycol. Res., 99, 441–446.
Hazarika D. K., Das K. K., 1998. Biological management of root rot of french bean (Phaseolus vulgaris L.) calised by Rhizoctonia solani. Plant Dis. Res., 13, 2, 101–105.
Indra N., Thribuvanamala G., 2002. Antagonisms of Trichoderma spp. against Macrophomina phaseolina causing root rot of bleck gram. Plant Dis. Res., 17, 1, 142–144.
Kumar S., Sharma S., Sharma B. K., Thakur D. P., 2005. Effect of culture filtrate of antagonists on Sclerospora graminicola sporangial formation and germination, India. Environ. Ecology, 23 (Special 4), 832–837.
Kücük C., Kivanc M., 2003. Isolation of Trichoderma spp. and Determination of Their Antifungal. Bioch. Physiol. Features. Turk. J. Biol., 27, 247–253.
Lin A., Lee T. M., Dern J. C., 1994. Tricholin, a new antifungal agent from Trichoderma viride and its action in biological control of Rhizoctonia solani. Antibiots, 47, 7, 799–805.
Liu H. Y., 1988. Hyperparasitism of Gliocladium deliquescens and Penicillium vermiculatum on Sclerotinia sclerotiorum. Plant Protect. Bull., Tajwan, 31, 175–184.
Łacicowa B., Pięta D., 1990. Skuteczność zaprawiania nasion fasoli Trichoderma koningii i Gliocladium catenulatum w warunkach zagrożenia chorobowego przez niektóre grzyby przeżywające w glebie. Phytopatologia Polonica, XI, 16–24.
Łacicowa B., Pięta D., 1994a. Ochronne działanie mikrobiologicznego zaprawiania nasion grochu (Pisum sativum L.) przeciwko chorobotwórczym grzybom odglebowym. Annales UMCS, Horticultura, s. EEE, II, 165–171.
Łacicowa B., Pięta D., 1994b. Skuteczność mikrobiologicznego zaprawiania nasion fasoli wielokwiatowej (Phaseoli coccineus L.) w warunkach zagrożenia chorobowego przez grzyby przeżywające w glebie. Annales UMCS, Horticultura, s. EEE, II, 157–164.
Matcalf D. A., Wilson C. R., 2001. The process of antagonisms of Sclerotium cepivorum in white rot affected onion roots by Trichoderma koningii. Plant Pathology, 50 (2), 249–257.
Mesta R. K., Amaresh Y. S., 2000. Biological control of Sclerotium wilt of sunflower. Plant Dis. Res., 15 (2), 202–203.
Mishra C. B. P., Behr L., 1976. Der Enfluss von Kulturfiltraten von Fusarium culmorum (W. G. Sm.) Sacc. Fusarium avenaceum (Fr.) Sacc. und Fusarium nivale (Fr.) Ces. (Griphosphaeria nivalis Müller et v. Arx) auf die Keimung des Weizen. Arch. Phytopathol., Pflanzenschutz., 12, 373–377.
Mukherjee P. K., Mukhopadhyay A. N., Sarmah D. K., Shrestha S. M., 1995. Comparative antagonistic properties of Gliocladium virens and Trichoderma harzianum on Sclerotium rolfsii and Rhizoctonia solani – it's relevance to understanding the mechanisms of biocontrol. J. Phytopathol., 143, 275–279.
Oktaba W., 1987. Metody statystyki matematycznej w doświadczalnictwie. PWN, Warszawa, 488 pp.
Papavizas G. S., 1985. Trichoderma and Gliocladium. Biology, ecology and potential for biocontrol. Ann. Rev. Phytopath., 23, 23–54.
Pastucha A., 1999. Ochronne działanie mikroorganizmów antagonistycznych w ograniczaniu występowania chorób soi (Glycine max (L.) Merrill). Annales UMCS, Horticultura, s. EEE, VII, 119–135.
Pastucha A., Patkowska E., 2005. Wpływ płynów pohodowlanych grzybów antagonistycznych na zdrowotność i plonowanie soi. Acta Agrobotanica, 58, 2, 111–124.
Pięta D., 1997. Niektóre aspekty wykorzystania mikroorganizmów antagonistycznych do zwalczania chorób roślin. Annales UMCS, Horticultura, s. EEE, V, 1–4.
Pięta D., 1998. Rola mikroorganizmów antagonistycznych w zwalczaniu chorób roślin. Rocz. AR w Poznaniu, CCCIV, Ogr., 27, 221–227.
Pięta D., Pastucha A., 2004. Biological methods of protecting common bean (Phaseolus vulgaris L.). Folia Univ. Agric. Stein. Agricultura, 239 (95), 301–306.
Pięta D., Patkowska E., Pastucha A., 1998. The efficiency of microbiological dressing of pea (Pisum sativum L.) against pathogenic soilborne fungi. Ann. Agric. Sci., E, Plant Protect., 27, ½, 81–89.
Pięta D., Patkowska E., Pastucha A., Bełkot M., 2002. Wpływ mikroorganizmów antagonistycznych na ograniczenie porażenia soi przez grzyby chorobotwórcze przeżywające w glebie. Acta Sci. Pol., Hortorum Cultus, 1 (1), 23–30.
Pięta D., Pastucha A., Patkowska E., 2003. The use of antagonistic microorganisms in biological control of bean diseases. Sodininkystè ir Darzininkystè Horticulture and Vegetable Growing, 22, 3, 401–406.
Prasad R. D., Rangeshwaran R., 2000. Effect of soil application of agranular formulation of Trichoderma harzianum on Rhizoctonia solani incited seed rot and damping-off of chickpea. J. Mycol. Plant Pathol., 30, 2, 216–220.
Rasami, Aggarwal, Srivastowa K. D., Singh D. V., 2001. Biological control of loose smuto f wheat: seed treatment with Trichoderma viride and its influence on plant growth. Ann. Plant Prot. Sci., 9, 1, 63–67.
Roberti R., de Vero L., Pisi A., Elad Y. (ed.), Freeman S. (ed), Monte E., 2001. Biological control of wheat root rot by antagonistic fungi and their modes of action. IOBC-WPRS Working Group “Biological Control of Fungal and Bacterial Plant Pathogens”. Proceeding of the sixth other Means of Control Sevilla Spain, November 30, December 3. Biull. OILB-SROP, 2001, 24, 3, 13–16.
Roberti R., Zakrisson E., Flaming F., de Vero L., 2002. Antagonistic fungi producing hydrolytic enzymes, active in degrading the cell wall of some root rot pathogens (Fusarium spp.) of wheat. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz, 109, 101–108.
Roznay Z. D., Kavesi M., Klement Z., Kajana L., 1991. Biological cancer and dieback disease of abricots. Acta Horticulture, 293, 525–532.
Shalini V., Dohroo N. P., 2003. Morphological, cultural and pathogenic variability in Fusarium oxysporum isolates form pea. Plant Dis. Res., 18 (1), 5–8.
Sharma M., Sharma S. K., 2001. Biological of Dematophora necatrix causing white root rot of apple. Plant Dis. Res., 16, 1, 40–45.
Singh Y., 1998. Biological control of Sclerotinia sclerotiorum . Plant Dis. Res., 13, 2, 144–146.
Stefanowa M., Leiva A., Larrinaga L., Coronado M. F., 1999. Metabolic activity of Trichoderma spp. isolates for a control of soilborne phytopathogenic fungi. Revista de la Facultad de Agronomia, Universiclad del Zulia, 16, 5, 509–516.
Weller D. M., 1998. Biological control of soilborne plant pathogens in the rhizosphere. Phytopathology, 26, 379–407.
Weller D. M., Raaijmakers J. M., Gardener B. B., Thomashow L. S., 2002. Responsible for specific soil suppression to plant pathogens. Ann. Rev. Phytopathology, 27, 502–509.
Benhamou N., Chet I., 1996. Parasitism of sclerotia of Sclerotium rolfsii by Trichoderma harzianum: Ultrastructural and cytochemical aspects of the interaction. Phytopathol., 86, 405–416.
Berg G., Roskot N., Steidle A., Eberl L., Zock A., Smalla K., 2002. Plant-diseases phenotypic diversity of antagonistic rhizobacteria isolated from different Verticillium dahliae Kleb. Appl. Environ. Microbiol., 68, 3328–3338.
Dubay S. C., 2002. Bio-agent based integrated management of collar rot of french bean. Indian Phytopathol., 55, 2, 230–231.
Elad J., 2000. Trichoderma harzianum T 39 preparation for biocontrol of plant diseases – control of Botrytis cinerea, Sclerotinia sclerotiorum and Cladosporium fulvum. Biocontrol Sci. and Technology, 10, 4, 499–507.
El-Kafrawy A. A., 2002. Biological control of bean damping of caused by Rhizoctonia solani Egiptian J. Agric. Res., 80, 1, 57–70.
Filipowicz A., 1983. Grzyby zasiedlające więdnący groch siewny oraz podatność na nie różnych odmian tej rośliny. Zesz. Prob. Post. Nauk Rol. 275, 107–120.
Filipowicz A., 1993. Fungi colonizing and decaying plants of pea (Pisum sativum L.). Phytopath. Pol., (XVIII), 6, 73.
Gherbawy Y. A. M. H., Yaser M., 2003. Fungicides and some biological controller agents effect on the growth of Fusarium oxysporum causing paprika wilt. Rev. Plant Pathol., 83 (5), 585.
Gurha S. N., 2001. Effect of some Trichoderma spp. on the growth of different isolates of Fusarium oxysporum f. sp. ciceris, in vitro. Ann. Plant Protect. Sci., 9, 2, 332–334.
Hagedorn D. J., 1989. Compedium of pea diseases. The Amerian Phytopath. Soc., USA.
Harman G. E., Hayes C. K., Lorito M., Di Pietro A., Brodway R. M., Tronsmo A., 1993. Chitinolytic enzymes produced by Trichoderma harzianum, purification of chitobiosidase. Phytopathology, 83, 313–318.
Haran S., Schickler H., Oppenheim A., Chet I., 1995. New components of the chitinolytic system of Trichoderma harzianum. Mycol. Res., 99, 441–446.
Hazarika D. K., Das K. K., 1998. Biological management of root rot of french bean (Phaseolus vulgaris L.) calised by Rhizoctonia solani. Plant Dis. Res., 13, 2, 101–105.
Indra N., Thribuvanamala G., 2002. Antagonisms of Trichoderma spp. against Macrophomina phaseolina causing root rot of bleck gram. Plant Dis. Res., 17, 1, 142–144.
Kumar S., Sharma S., Sharma B. K., Thakur D. P., 2005. Effect of culture filtrate of antagonists on Sclerospora graminicola sporangial formation and germination, India. Environ. Ecology, 23 (Special 4), 832–837.
Kücük C., Kivanc M., 2003. Isolation of Trichoderma spp. and Determination of Their Antifungal. Bioch. Physiol. Features. Turk. J. Biol., 27, 247–253.
Lin A., Lee T. M., Dern J. C., 1994. Tricholin, a new antifungal agent from Trichoderma viride and its action in biological control of Rhizoctonia solani. Antibiots, 47, 7, 799–805.
Liu H. Y., 1988. Hyperparasitism of Gliocladium deliquescens and Penicillium vermiculatum on Sclerotinia sclerotiorum. Plant Protect. Bull., Tajwan, 31, 175–184.
Łacicowa B., Pięta D., 1990. Skuteczność zaprawiania nasion fasoli Trichoderma koningii i Gliocladium catenulatum w warunkach zagrożenia chorobowego przez niektóre grzyby przeżywające w glebie. Phytopatologia Polonica, XI, 16–24.
Łacicowa B., Pięta D., 1994a. Ochronne działanie mikrobiologicznego zaprawiania nasion grochu (Pisum sativum L.) przeciwko chorobotwórczym grzybom odglebowym. Annales UMCS, Horticultura, s. EEE, II, 165–171.
Łacicowa B., Pięta D., 1994b. Skuteczność mikrobiologicznego zaprawiania nasion fasoli wielokwiatowej (Phaseoli coccineus L.) w warunkach zagrożenia chorobowego przez grzyby przeżywające w glebie. Annales UMCS, Horticultura, s. EEE, II, 157–164.
Matcalf D. A., Wilson C. R., 2001. The process of antagonisms of Sclerotium cepivorum in white rot affected onion roots by Trichoderma koningii. Plant Pathology, 50 (2), 249–257.
Mesta R. K., Amaresh Y. S., 2000. Biological control of Sclerotium wilt of sunflower. Plant Dis. Res., 15 (2), 202–203.
Mishra C. B. P., Behr L., 1976. Der Enfluss von Kulturfiltraten von Fusarium culmorum (W. G. Sm.) Sacc. Fusarium avenaceum (Fr.) Sacc. und Fusarium nivale (Fr.) Ces. (Griphosphaeria nivalis Müller et v. Arx) auf die Keimung des Weizen. Arch. Phytopathol., Pflanzenschutz., 12, 373–377.
Mukherjee P. K., Mukhopadhyay A. N., Sarmah D. K., Shrestha S. M., 1995. Comparative antagonistic properties of Gliocladium virens and Trichoderma harzianum on Sclerotium rolfsii and Rhizoctonia solani – it's relevance to understanding the mechanisms of biocontrol. J. Phytopathol., 143, 275–279.
Oktaba W., 1987. Metody statystyki matematycznej w doświadczalnictwie. PWN, Warszawa, 488 pp.
Papavizas G. S., 1985. Trichoderma and Gliocladium. Biology, ecology and potential for biocontrol. Ann. Rev. Phytopath., 23, 23–54.
Pastucha A., 1999. Ochronne działanie mikroorganizmów antagonistycznych w ograniczaniu występowania chorób soi (Glycine max (L.) Merrill). Annales UMCS, Horticultura, s. EEE, VII, 119–135.
Pastucha A., Patkowska E., 2005. Wpływ płynów pohodowlanych grzybów antagonistycznych na zdrowotność i plonowanie soi. Acta Agrobotanica, 58, 2, 111–124.
Pięta D., 1997. Niektóre aspekty wykorzystania mikroorganizmów antagonistycznych do zwalczania chorób roślin. Annales UMCS, Horticultura, s. EEE, V, 1–4.
Pięta D., 1998. Rola mikroorganizmów antagonistycznych w zwalczaniu chorób roślin. Rocz. AR w Poznaniu, CCCIV, Ogr., 27, 221–227.
Pięta D., Pastucha A., 2004. Biological methods of protecting common bean (Phaseolus vulgaris L.). Folia Univ. Agric. Stein. Agricultura, 239 (95), 301–306.
Pięta D., Patkowska E., Pastucha A., 1998. The efficiency of microbiological dressing of pea (Pisum sativum L.) against pathogenic soilborne fungi. Ann. Agric. Sci., E, Plant Protect., 27, ½, 81–89.
Pięta D., Patkowska E., Pastucha A., Bełkot M., 2002. Wpływ mikroorganizmów antagonistycznych na ograniczenie porażenia soi przez grzyby chorobotwórcze przeżywające w glebie. Acta Sci. Pol., Hortorum Cultus, 1 (1), 23–30.
Pięta D., Pastucha A., Patkowska E., 2003. The use of antagonistic microorganisms in biological control of bean diseases. Sodininkystè ir Darzininkystè Horticulture and Vegetable Growing, 22, 3, 401–406.
Prasad R. D., Rangeshwaran R., 2000. Effect of soil application of agranular formulation of Trichoderma harzianum on Rhizoctonia solani incited seed rot and damping-off of chickpea. J. Mycol. Plant Pathol., 30, 2, 216–220.
Rasami, Aggarwal, Srivastowa K. D., Singh D. V., 2001. Biological control of loose smuto f wheat: seed treatment with Trichoderma viride and its influence on plant growth. Ann. Plant Prot. Sci., 9, 1, 63–67.
Roberti R., de Vero L., Pisi A., Elad Y. (ed.), Freeman S. (ed), Monte E., 2001. Biological control of wheat root rot by antagonistic fungi and their modes of action. IOBC-WPRS Working Group “Biological Control of Fungal and Bacterial Plant Pathogens”. Proceeding of the sixth other Means of Control Sevilla Spain, November 30, December 3. Biull. OILB-SROP, 2001, 24, 3, 13–16.
Roberti R., Zakrisson E., Flaming F., de Vero L., 2002. Antagonistic fungi producing hydrolytic enzymes, active in degrading the cell wall of some root rot pathogens (Fusarium spp.) of wheat. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz, 109, 101–108.
Roznay Z. D., Kavesi M., Klement Z., Kajana L., 1991. Biological cancer and dieback disease of abricots. Acta Horticulture, 293, 525–532.
Shalini V., Dohroo N. P., 2003. Morphological, cultural and pathogenic variability in Fusarium oxysporum isolates form pea. Plant Dis. Res., 18 (1), 5–8.
Sharma M., Sharma S. K., 2001. Biological of Dematophora necatrix causing white root rot of apple. Plant Dis. Res., 16, 1, 40–45.
Singh Y., 1998. Biological control of Sclerotinia sclerotiorum . Plant Dis. Res., 13, 2, 144–146.
Stefanowa M., Leiva A., Larrinaga L., Coronado M. F., 1999. Metabolic activity of Trichoderma spp. isolates for a control of soilborne phytopathogenic fungi. Revista de la Facultad de Agronomia, Universiclad del Zulia, 16, 5, 509–516.
Weller D. M., 1998. Biological control of soilborne plant pathogens in the rhizosphere. Phytopathology, 26, 379–407.
Weller D. M., Raaijmakers J. M., Gardener B. B., Thomashow L. S., 2002. Responsible for specific soil suppression to plant pathogens. Ann. Rev. Phytopathology, 27, 502–509.
Danuta Pięta
University of Life Sciences in Lublin
University of Life Sciences in Lublin
Alina Pastucha
University of Life Sciences in Lublin
University of Life Sciences in Lublin
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