AN EFFECTIVE PROTOCOL FOR IN VITRO GERMINATION AND SEEDLING DEVELOPMENT OF LENTISK (PISTACIA LENTISCUS L.)
Hakan Yıldırım
Malatya Turgut Ozal University, Faculty of Agriculture, Department of Horticulture, Malatya, TurkeyNazan Çalar
Dicle University, Faculty of Science, Department of Biology, 21280 Diyarbakir, TurkeyAhmet Onay
Dicle University, Faculty of Science, Department of Biology, Diyarbakir, TurkeyAbstract
Different nutrient media (MS [Murashige and Skoog 1962]; QL [Quoirin and Lepoivre 1977] and WPM [Lloyd and McCown 1980]); plant growth regulators BA (benzil adenin), GA3 (gibberellic acid), IBA (indole-3-butyric-acid), NAA (naftalen acedic acid); and sucrose concentrations were studied to determine the in vitro culture effects on healthier and faster seedling development from mature lentisk (Pistacia lentiscus L.) seeds. After 28 days of culture, the percentage of germinated seeds was the highest (70%) in the full-strength MS medium. The cytokinin BA was superior to other tested treatments in terms of its ability to promote germination of lentisk seeds. When tested at different concentrations, sucrose gave the best results obtained at concentrations of 1–4%, whereas high concentrations (6 and 8%) mainly decreased germination rate and there was no a regular pattern for elongation of the aerial parts of plants. With this described protocol, on average 76.67% seeds germinated 4 weeks after culture. Developed seedlings were satisfactorily acclimatized in sterilized peat, soil and perlite containing compost, with high percentage survival viability was obtained 9 months after transfer to in vivoconditions (93.33%). The results obtained showed that the enriched full-strength MS medium supplemented with 1 mg L–1 BA and 3% sucrose induced homogeneous and healthy seedling development in a period of 4 to 8 weeks of culture.
Keywords:
mastic tree, micropropagation, seedReferences
Abousalim, A., El Mahboul, B., Walali, L.D. (1992). Germination in vitro de graines et croissance de plantules de pistachier (Pistacia vera L.). Rev. Réseau Amélior. Product. Agric. Milieu Aride, 4(17), 23.
Ayfer, M., Serr, E.F. (1961). Effects of gibberellin and other factors and seed germination and early growth in Pistacia species. J. Am. Soc. Hortic. Sci., 77(308), 315.
Bewley, J.D., Black, M. (1994). Seeds: physiology of development and germination. Plenum Press, New York–London, 445.
Crane, J.C., Forde, H.I. (1974). Improved Pistacia seed germination. Calif. Agric., 28(9), 8–9.
Fascella, G., Airò, G., Zizzo, G.V., Ruffoni, B. (2004). Prime osservazioni sula coltivazione in vitro di Lentisco (Pistacia lentiscus L.). Italus Hortus, 11(4), 141–143.
Garcia-Fayos, P., Lolina, M.J. (1990). Estructura del Mattoral mediterraneo en relacion con un gradient de ladera. Stud. Oecol., 7, 19–31.
Garcia-Fayos, P., Verdu, M. (1998). Soil seed bank, factors controlling germination and establishment of a Mediterranean shrub: Pistacia lentiscus L. Acta Oecol., 19(4), 357–366.
Grundwag, M. (1976). Embryology and fruit development in four species of Pistacia (Anacardiaceae). Bot. J. Linn. Soc., 73, 355–370.
İsfendiyaroğlu, M., Özeker, E. (2001). The relation between phenolic compounds and seed dormancy in Pistacia spp. Cah. Options Méditterr., 56, 227–232.
Jubes, J.T., Martinez, H., Padilla, E., Oste, C.A. (1975). Effects of mechanical scarification, substrate, seed position and gibberellic acid on germination of Cherimoya. Rev. Agron. Noroeste Arg., 12, 161–172.
Karakır, M.N., İsfendiyaroğlu, M. (1999). Sakız ağacı (Pistacia lentiscus L.)’nın vegetatif yöntemlerle çoğaltılması ve kök oluşumunun anatomik-fizyolojik ıncelenmesi üzerine araştırmalar. Tübitak, TOGTAG-1511 Sonuç Raporu, Izmir, 97 s.
Koç, İ, Onay, A., Çiftçi, YÖ. (2014). In vitro regeneration and conservation of the lentisk (Pistacia lentiscus L.). Turk. J. Biol., 38, 653–663. DOI:10.3906/biy-1401-69.
Lloyd, G., McCown, B. (1980). Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Comb. Proc. Int. Plant Prop. Soc., 30, 421–427.
Maestre, F.T., Cortina, J. (2005). Remnant shrubs in Mediterranean semi-arid steppes effect of shrub size, abiotic factors and species identity on understorey richness and occurrence. Acta Oecol, 27, 161–169.
Mascarello, C., Fascella, G., Zizzo, G.V., Mantovani, E, Ruffoni, B. (2007). In vivo and in vitro propagation of Pistacia lentiscus L. ISHS Acta Hortic., 764, 299–306.
Mulas, M., Abeltino, P., Brigaglia, N. (1998). Evaluation of Pistacia lentiscus L. Genetic resources to select ecotypes having high efficiency in the colonization of Marginal Lands. Acta Hortic., 457, 279–286.
Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15, 473–479.
Kaşka, N., Ak, B.E., Nikpeyma, Y. (1992). Antep fıstıklarında tüplü fidan üretimi üzerinde bir ön araştırma. Türkiye I. Ulusal Bahçe Bitkileri Kongresi, 79–83.
Özbek, S., Ayfer, M. (1959). Türkiye’de Antep fıstığı (Pistacia vera L.) anaçları ve aşı tekniği. AÜ Zir. Fak. Yayınları, 14(4), 189–214.
Piotto, B. (1995). Influence of scarification and prechilling on the germination of seeds of Pistacia lentiscus. Seed Sci. Technol., 23, 659–663.
Quoirin, M., Lepoivre, P. (1977). Étude des milieux adaptés aux cultures in vitro de Prunus. Acta Hortic., 78, 437–442.
Ruffoni, B., Mascarello, C., Fascella, G., Airò, M., Amoretti, M. (2004). La micropropagazione del Lentisco (Pistacia lentiscus L.), Atti II Convegno nazionale “Piante mediterranee: Valorizzazione delle risorse e sviluppo sostenibile”. Agrigento, 7–8 ottobre, 277–280.
Taşkın, T., İnal, A. (2005). Sakız Ağacı (Pistacia lentiscus var chia Duhamel)’nın in vitro mikroçoğaltımı üzerine araştırmalar. Anadolu J. AARI, 15(1), 1–14.
Tombolato, A., Monet, R. (1984). Utilisation d’axes embryonnaires de pêcher pour apprécier l’effet d’un milieu nutritif minéral sur le développement des tiges ou des racines en culture in vitro. Agronomie, 4(10), 927–931.
Vargas, M.E. (1986). Efecto del ácido giberélico y 6-ben- cil-amino-purina sobre la germinación de semillas de chirimoyo (Annona cherimola Mill.) cv. Bronceada. Tesis Ingeniero Agronomo, Universidad Catolica de Valpariso, Facultad de Agronomia, Chile.
Yıldırım, H. (2012). Micropropagation of Pistacia lentiscus L. from axenic seedling-derived explants. Sci. Hortic., 137, 29–35.
Yıldırım, H., Tilkat, E., Onay, A., Ozen, H.Ç. (2007). In vitro embryo culture of apricot (Prunus armeniaca L.) cv. hacıhaliloglu. Int. J. Sci. Technol., 2(2), 99–104.
Zohary, M.A. (1952). Monographic study of the genus Pistacia. Palest. J. Bot., 5(4), 187–226.
Malatya Turgut Ozal University, Faculty of Agriculture, Department of Horticulture, Malatya, Turkey
Dicle University, Faculty of Science, Department of Biology, 21280 Diyarbakir, Turkey
Dicle University, Faculty of Science, Department of Biology, Diyarbakir, Turkey
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