Senescence o cut leaves of Zantedeschia aethiopica and Z. elliottiana. Part I. Chlorophyll degradation
Ewa Skutnik,Warsaw Agricultural University
Julita Rabiza-Świder,Warsaw Agricultural University
Mariusz WachowiczWarsaw Agricultural University
Aleksandra ŁukaszewskaWarsaw Agricultural University
Chlorophyll degradation occurring during leaf senescence in under control of plant hormones. Changes in the chlorophyll content and the effects of BA (benzyladenine) and GA3 (gibberellic acid) on this process were analyzed during senescence of cut leaves of Zantedeschia aethiopica Spr. and Zantedeschia elliottiana Engl., two species grown for the florists’ green. Both growth regulators were applied as 24 h pulse treatment: 0.25 mmol·dm-3 and 0.1 mmol·dm-3 for GA3 and BA, respectively. Gibberellic acid was more effective than benzyladenine in delaying senescence of both Zantedeschia species leaves by retarding chlorophyll loss. A standard preservative solution used to prolong longevity of cut flowers (8-HQC /citrate of hydroxyquinoline/ +2% S /sucrose/) radically accelerated chlorophyll loss of Z. aethiopica leaves but had no effect on pigment degradation in leaves of Z. elliottiana. However, in both species the preservative diminished the positive effect of GA3 treatment on final chlorophyll content.
Keywords:chlorophyll degradation, cut leaves, florists’ green, gibberellic acid, benzyladenine, Zantedeschia aethiopica, Zantedeschia elliottiana
Downs C. G., Somerfield S. D., Davey M. C., 1997. Cytokinin treatment delays senescence but not sucrose loss in harvested broccoli. Postharvest Biol. Technol. 11, 93–100.
Fang Z., Bouwkamp J. C., Solomos T., 1998. Chlorophyllase activities and chlorophyll degradation during leaf senescence in non-yellowing mutant and wild type of Phaseolus vulgaris L. J. Exp. Bot. 49, 503–510.
Gan S., Amasino R., 1997. Making sense of senescence. Plant Physiol. 102, 311–319.
Guak S., Fuchigami L. H., 2002. Foliar application of urea or ABA affect growth cessation, leaf senescence and abscission, cold acclimation and levels of reserve nitrogen and carbohydrates in nitrogen-treated apple nursery plants. J. Hort. Sci. Biotechn. 77, 137–142.
Halevy A. H., Mayak S., 1981. Senescence and postharvest physiology of cut flowers. Hort. Rev. 3, 59–143.
Hodges D. M., Forney C. F., 2000. The effect of ethylene, depressed oxygen and elevated carbon dioxide on antioxidant profiles of senescing spinach leaves. J. Exp. Bot. 51, 645–655.
Kappers I. F., Jordi W., Tsesmetzis N., Maas F. M., Van der Plas L. H. W., 1998. GA4 does not require conversion into GA1 to delay senescence of Alstroemeria hybrida leaves. J. Plant Growth Regul. 17, 89–93.
Lu C., Lu Q., Zhang J., Kuang T., 2001. Characterization of photosynthetic pigment composition, photosystem II photochemistry and thermal energy dissipation during leaf senescence of wheat grown in the field. J. Exp. Bot. 52, 1805–1810.
Masclaux C., Valadier M. H., Brugiere N., Morot-Gaudry J. F., Hirel B., 2000. Characterization of the sink/source transition in tobacco (Nicotiana tabacum L.) shoots in relation to nitrogen management and leaf senescence. Planta 211, 510–518.
Matile P., Hörtensteiner S., Thomas P., Kräutler B., 1996. Chlorophyll breakdown in senescent leaves. Plant Physiol. 112, 1403–1409.
Moran R., Porath D., 1980. Chlorophyll determination in intact tissues using N,N-Dimethylformamide. Plant Physiol. 65, 478–479.
Pic E., Teyssendier de la Serve B., Tardieu F., Turc O., 2002. Leaf senescence induced by mild water stress deficit follows the same sequence of macroscopic, biochemical, and molecular events as monocarpic senescence in pea. Plant Physiol. 128, 236–246.
Rabiza-wider J., Rybka Z., Skutnik E., Łukaszewska A., 2003. Proteolysis and expression of the cysteine protease gene in senescing cut leaves of Hosta ‘Undulata Erromena’ and Zantedeschia aethiopica Spr. treated with BA or GA3. Acta Physiol. Plant. 25, 319–324.
Rossato L., MacDuff J. H., Laine P., Le Deunff E., Ourry A., 2002. Nitrogen storage and remobilization in Brassica napus L. during the growth cycle: Effects of methyl jasmonate on nitrate uptake, senescence, growth, and VSP accumulation. J. Exp. Bot. 53, 1131–1141.
Scheumann V., Schoch S., Rüdiger W., 1999. Chlorophyll b reduction during senescence of barley seedlings. Planta 209, 364–370.
Skutnik E., Łukaszewska A., Tyborowska K., 1999. Retarding senescence of cut leaves of Hosta plantaginea by growth regulators. Annals of Warsaw Agricultural University – SGGW Horticulture, Landscape Architecture 20, 3–8.
Skutnik E., Łukaszewska A., 2001. Regulacja pozbiorczej trwałości gatunków uprawianych na zieleń ciętą. Post. Nauk Roln. 5, 111–124.
Skutnik E., Łukaszewska A., Serek M., Rabiza J., 2001. Effect of growth regulators on postharvest characteristics of Zantedeschia aethiopica. Postharvest Biol. Technol. 21, 241–246.
Smart C., 1994. Gene expression during leaf senescence. New Phytologist 126, 419–448.
Thomas H., Stoddart J. L., 1980. Leaf senescence. Ann. Rev. Plant Physiol. 31, 83–111.
Thomas H., Ougham H., Canter P., Donnison I., 2002. What stay-green mutants tell us about nitrogen remobilization in leaf senescence. J. Exp. Bot. 53, 801–808.
Wingler A., von Schaewen A., Leegood R. C., Lea P. J., Quick W. P., 1998. Regulation of leaf senescence by cytokinin, sugars, and light. Plant Physiol. 116, 329–335.
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