Characteristics and distribution of pigments in flowers of the new interspecific hybrid

Irena Olszewska-Kaczyńska

Warsaw University of Life Sciences

Henryk Chmiel

Warsaw University of Life Sciences


Abstract

Hippeastrum × chmielii Abstract. Five clones of a new interspecific hybrid Hippeastrum × chmielii Chm. obtained by crossing Hippeastrum hybridum with Hippeastrum pratense were compared. Content and distribution of chlorophylls, carotenoids and anthocyanins were determined in fresh perianth leaves using spectrophotometric method and calculated on dry mass basis. Separation and identification of anthocyanidins were done by TLC on cellulose plates against standards. Variations in intensity and shade of perianth leaves colour were due to differences in content and distribution as well as copigmentation in three studied classes of the pigments. 

Keywords:

Hippeastrum × chmielii, pigments, carotenoids, anthocyans, chlorophylls, TLC – thin layer chromatography

Chmiel H., Mynett K., 1997. Nowe odmiany mieszańcowe Hippeastrum otrzymane w Katedrze Roslin Ozdobnych SGGW. Biuletyn SPORC 6, 55–66.
Chmiel H., 2000. Uprawa roślin ozdobnych. PWRiL Warszawa
Elbanowska A., Napierała A., 1989. Antocyjany w owcach Aronia melanocarpa. Herba polonica 35 (4), 188–189.
Fuleki F., Francis F. J., 1968. Quantitative methods for anthocyanins 1. Extraction and determination of total anthocyanin in cranberries. J. Food Sci. 33, 72–77.
Griesbach R. J., Batdorf L., 1995. Flower Pigments within Hemerocallis fulva fm. fulva, fm. rosea and fm. disticha. Hort Science 30(2), 353–354.
Hwang Y-J., Yoshikawa K., Miyajima I., Okubo H., 1992. Flower colors and pigments in hybrids with Camellia chrysantha. Sci. Hort. 51 (3–4): 251–260
Lichtenthaler H. K., Wellburn A. R., 1983. Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochemical Societty Transaction 603 meeting Liverpool, 591–592.
Martha R., Weiss, 1995. Floral color change: A widespread functional convergence. Am. J. Bot. 82 (2), 167–185.
Saito N., Harborne J. B., 1992. Correlations between anthocyanin type, pollinator and flower colour in the Labiatae. Phytochemistry 31, 3009–3015.
The Royal Horticultural Society Colour Chart, 1966. London. Yamaguci A. A., Terahara N., Shizukuishi K., 1990. Acylated anthocyanins in Zinnia elegans flowers. Phytochemistry 29(4), 1269–1270.

Published
2002-12-31



Irena Olszewska-Kaczyńska 
Warsaw University of Life Sciences
Henryk Chmiel 
Warsaw University of Life Sciences



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