Comparative analysis of the biotechnological potential of Knautia drymeia Heuff. and K. macedonica Griseb

Małgorzata Chrząszcz

Department of Pharmaceutical Botany, Medical University of Lublin, Poland

Katarzyna Dos Santos Szewczyk

Department of Pharmaceutical Botany, Medical University of Lublin, Poland

Agnieszka Dąbrowska

Botanical Garden of Maria Curie-Skłodowska University, Lublin, Poland

Dorota Tchórzewska

Department of Cell Biology, Institute of Biological Sciences, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland


The present study of Knautia drymeia and K. macedonica is in line with the current trend of searching for new plant species that can potentially be used as medicinal herb materials. A comparative analysis of the morphological and anatomical structure of both species was performed together with the distribution of polyphenolic compounds, which was correlated with the tissue structure of plant organs. Quantitative phytochemical analyses were performed to supplement the biophysical analyses. Both species had a similar morphological, anatomical, and histological structure. Polyphenolic compounds were accumulated in the parenchyma tissue in an organ-specific mode, mainly in the leaves. The phytochemical analyses revealed organ- and species-dependent variations in the polyphenol content. Thus, the highest polyphenol amount was observed in the leaves, with equal levels of total polyphenols and phenolic acids in the leaves of K. macedonica and K. drymeia, respectively. The present study integrates morphological/histological analyses with investigations of the biotechnological/pharmaceutical potential of the studied plants and constitutes an innovative and holistic approach to the current research problem.


Knautia, morphology, histology, phenolic compounds, flavonoids, phenolic acids

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Małgorzata Chrząszcz 
Department of Pharmaceutical Botany, Medical University of Lublin, Poland
Katarzyna Dos Santos Szewczyk 
Department of Pharmaceutical Botany, Medical University of Lublin, Poland
Agnieszka Dąbrowska 
Botanical Garden of Maria Curie-Skłodowska University, Lublin, Poland
Dorota Tchórzewska 
Department of Cell Biology, Institute of Biological Sciences, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland


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