Functional characterization of ZjPYL8 from sour jujube: enhancing the sensitivity of stomata and roots to ABA in Arabidopsis thaliana
Peiyan Wang
1 Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy, Shijiazhuang, Hebei 050200, China; 2 College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, Chinahttps://orcid.org/0000-0001-9535-4861
Lanting Qi
College of Life and Environment Science, Minzu University of China, Beijing 100081, Chinahttps://orcid.org/0000-0003-4400-2035
Junna Song
1 Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy, Shijiazhuang, Hebei 050200, China; 2 College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, Chinahttps://orcid.org/0009-0001-1165-5964
Ruojia Zhu
1 Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy, Shijiazhuang, Hebei 050200, China; 2 College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, Chinahttps://orcid.org/0009-0009-5029-109X
Xiaowei Han
1 Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy, Shijiazhuang, Hebei 050200, China; 2 College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, Chinahttps://orcid.org/0000-0002-9445-2299
Yu Liu
College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, Chinahttps://orcid.org/0009-0008-1754-6954
Xianyun Wang
Cell Therapy Laboratory, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei 050031, Chinahttps://orcid.org/0000-0003-2224-4770
Yuguang Zheng
1 Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy, Shijiazhuang, Hebei 050200, China; 2 College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, Chinahttps://orcid.org/0000-0003-3411-5191
Zhao Liu
1 Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy, Shijiazhuang, Hebei 050200, China; 2 College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, Chinahttps://orcid.org/0009-0001-3408-966X
Abstract
Abscisic acid (ABA) is a plant hormone that plays a crucial role in regulating plant growth, development, and adaptation to stress. The growth of Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. Chou, commonly known as Suanzao in Chinese, is significantly influenced by environmental factors, particularly drought and salt stresses. In this study, we isolated and characterized a putative ABA receptor, ZjPYL8, from Sour jujube. To investigate the effects of ZjPYL8 overexpression on ABA-responsive pathways, we introduced it into Arabidopsis thaliana (A. thaliana) and examined the resulting phenotypes. Our results demonstrated that overexpression of ZjPYL8 in A. thaliana led to a significant reduction in stomatal aperture and root length under ABA treatment, while the wild type (WT) was relatively insensitive to ABA. Moreover, ZjPYL8 transgenic plants exhibited shorter roots under salt treatment than the WT did. These findings suggest that the overexpression of ZjPYL8 in A. thaliana enhances the plant's resistance to stress and support the hypothesis that ZjPYL8 serves as a putative ABA receptor in Sour jujube, which may improve the plant's adaptability to drought and salt stresses. ZjPYL8 appears to mediate plant responses to ABA, similar to most ABA receptors in A. thaliana, such as stomatal closure and root length.
Keywords:
ZjPYL8, osmotic stress, salt stress, abscisic acid, sour jujubeReferences
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1 Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy, Shijiazhuang, Hebei 050200, China; 2 College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, China https://orcid.org/0000-0001-9535-4861
College of Life and Environment Science, Minzu University of China, Beijing 100081, China https://orcid.org/0000-0003-4400-2035
1 Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy, Shijiazhuang, Hebei 050200, China; 2 College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, China https://orcid.org/0009-0001-1165-5964
1 Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy, Shijiazhuang, Hebei 050200, China; 2 College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, China https://orcid.org/0009-0009-5029-109X
1 Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy, Shijiazhuang, Hebei 050200, China; 2 College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, China https://orcid.org/0000-0002-9445-2299
College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, China https://orcid.org/0009-0008-1754-6954
Cell Therapy Laboratory, The First Hospital of Hebei Medical University, Shijiazhuang, Hebei 050031, China https://orcid.org/0000-0003-2224-4770
1 Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy, Shijiazhuang, Hebei 050200, China; 2 College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, China https://orcid.org/0000-0003-3411-5191
1 Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province, College of Pharmacy, Shijiazhuang, Hebei 050200, China; 2 College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, Hebei 050200, China https://orcid.org/0009-0001-3408-966X
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