EVALUATION OF GENETIC VARIABILITY WITHIN SWEET CHERRY (Prunus avium L.) GENETIC RESOURCES BY MOLECULAR SSR MARKERS
Josef Patzak
Hop Research Institute, Žatec, Czech Republic Research and BreedingAlena Henychová
Hop Research Institute, Žatec, Czech Republic Research and BreedingFrantišek Paprštein
Institute of Pomology, Holovousy, Czech RepublicJiří Sedlák
Institute of Pomology, Holovousy, Czech RepublicAbstract
Sweet cherry is a vegetatively propagated, perennial plant with high level of heterozygosity and ancient breeding history. Therefore, it is necessary to keep, conserve and evaluate known genetic resources for fu- ture breeding programs and fruit production stability. In present, the utilization of DNA molecular genetic analyses is the best suitable method for evaluation of individual accessions, thus we eliminated duplications and characterized the genetic relationships. In our work, we used PCR primer combinations for 19 SSR and 2 EST-SSR loci for analyses of 123 current, old and local sweet cherry cultivars from Czech genetic resources of Research and Breeding Institute of Pomology in Holovousy. In total, 115 polymorphic fragments were amplified, which we used for hierarchical cluster analysis of genetic variability. The result dendrograms were divided into three main clusters and ten subgroups. Clustering corresponded to genealogical and geobotanical characteristics of individual accessions as breeding history of several known accessions.
Keywords:
sweet cherry (Prunus avium L.), SSR and EST-SSR markers, genetic diversity, cultivars, hierarchical cluster analysisReferences
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Testolin, R., Marrazzo, T., Cipriani, G., Quarta, R., Verde, I., Dettori, M.T., Pancaldi, M., Sansavini, S. (2000). Microsatellite DNA in peach (Prunus persica L. Batch) and its use in fingerprinting and testing the genetic origin of cultivars. Genome, 43, 512–520. DOI: 10.1139/g00-010
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Vaughan, S.P., Russell, K. (2004). Characterization of novel microsatellites and development of multiplex PCR for large-scale population studies in wild cherry, Prunus avium. Mol. Ecol. Notes, 4, 429–431. DOI: 10.1111/j.1471-8286.2004.00673.x
Wünsch, A. (2009). Cross-transferable polymorphic SSR loci in Prunus species. Sci. Hortic., 120, 348–352. DOI: 10.1016/j.scienta.2008.11.012
Clarke, J.B., Tobutt, K.R. (2003). Development and characterization of polymorphic microsatellites from Prunus avium ‘Napoleon’. Mol. Ecol. Notes, 3, 578–580. DOI: 10.1046/j.1471-8286.2003.00517.x
De Rogatis, A., Ferrazzini, D., Ducci, F. Guerri, S., Carnevale, S., Belletti, B. (2013). Genetic variation in Italian wild cherry (Prunus avium L.) as characterized by nSSR markers. Forestry, 86, 391–400. DOI: 10.1093/forestry/cpt009
Dirlewanger, E., Cosson, P., Tavaud, M., Aranzana, M.J., Poizat C., Zanetto, A., Arús, P., Laigret, R. (2002). Development of microsatellite markers in peach (Prunus persica (L.) Batsch) and their use in genetic diversity analysis in peach and sweet cherry (Prunus avium L.). Theor. Appl. Genet., 105, 127–138. DOI: 10.1007/s00122-002-0867-7
Fernandez i Marti, A., Athanson, B., Koepke, T., Forcada, C.F., Dhingra, A, Oraguzie, N. (2012). Genetic diversity and relatedness of sweet cherry (Prunus avium L.) cultivars based on single nucleotide polymorphic markers. Front. Plant Sci., 3, 116. DOI: 10.3389/fpls.2012.00116
Ganopoulos, I., Tsaballa, A., Xanthopoulou, A., Madesis, P., Tsaftaris, A. (2013). Sweet Cherry Cultivar Identification by High-Resolution-Melting (HRM) Analysis Using Gene-Based SNP Markers. Plant Mol. Biol. Rep., 31, 763–768. DOI: 10.1007/s11105-012-0538-z.
Gasic, K., Han, Y., Kertbundit, S., Shulaev, V., Iezzoni, A.F., Stover, E.W., Bell, R.L., Wisniewski, M.E., Korban, S.S. (2009). Characteristics and transferability of new apple EST-derived SSRs to other Rosaceae species. Mol. Breed., 23, 397–411. DOI: 10.1007/s11032-008-9243-x
Goulao, L., Cabrita, C.M., Oliviera, C.M, Leitao, J.M. (2001). Comparing RAPD and AFLP analysis in discrimination and estimation of genetic similarities among apple (Malus × domestica Borkh.) cultivars. Euphytica, 119, 259–270. DOI: 10.1023/A:1017519920447
Lacis, G., Rashal, I., Ruisa, S., Trajkovski, V., Iezzoni, A.F. (2009). Assessment of genetic diversity of Latvian and Swedish sweet cherry (Prunus avium L.) genetic resources collections by using SSR (microsatellite) markers. Scientia Hortic., 121, 451–457. DOI: 10.1016/j.scienta.2009.03.016
Lacis, G., Rashal, I., Trajkovski, V. (2010). Comparative analysis of sweet cherry (P. avium) genetic diversity revealed by two methods of SSR marker detection. Proceedings of the Latvian Academy of Sciences, Section B. Natural, Exact, and Applied Sciences, 64, 149–158. DOI: 10.2478/v10046-010-0024-7
Mariette, S., Tavaud, M., Arunyawat, U., Capdeville, G., Millan, M., Salin, F. (2010). Population structure and genetic bottleneck in sweet cherry estimated with SSRs and the gametophytic self-incompatibility locus. BMC Genetics, 11, 77. DOI: 10.1186/1471-2156-11-77
Olmstead, J.W., Sebolt, A.M., Cabrera, A., Sooriyapathirana, S.S., Hammar, S., Iriarte, G., Wang, D., Chen, C.Y., Van Der Knaap, E., Iezzoni, A.F. (2008). Construction of an intra-specific sweet cherry (Prunus avium L.) genetic linkage map and synteny analysis with the Prunus reference map. Tree Genet. Genomes, 4, 897–910. DOI: 10.1007/s11295-008-0161-1
Paprštein, F., Kloutvor, J. (2015). Historical cultivars of sweet and sour cherries grown in territory of the Czech Republic. Research and Breeding Institute of Pomology Holovousy Ltd., Holovousy, 165 pp.
Patzak, J. (2001). Comparison of RAPD, STS, ISSR and AFLP molecular methods used for assessment of genetic diversity in hop (Humulus lupulus L.). Euphytica, 121, 9–18. DOI: 10.1023/A:1012099123877
Raymond, M., Rousset, F. (1995). GENEPOP, version 1.2: population genetics sofware for exact tests and ecuminicism. J. Hered., 86, 248–249.
Struss, D., Ahmad, R., Southwick, S.M., Boritzki, M. (2003). Analysis of sweet cherry (Prunus avium L.) cultivars using SSR and AFLPmarkers. J. Am. Soc. Hortic. Sci., 128, 904–909.
Testolin, R., Marrazzo, T., Cipriani, G., Quarta, R., Verde, I., Dettori, M.T., Pancaldi, M., Sansavini, S. (2000). Microsatellite DNA in peach (Prunus persica L. Batch) and its use in fingerprinting and testing the genetic origin of cultivars. Genome, 43, 512–520. DOI: 10.1139/g00-010
Turkoglu, Z., Bilgener, S., Ercisli, S., Yildirim, N. (2012). Simple sequence repeat (SSR) analysis for assessment of genetic variability in wild cherry germplasm. J. Appl. Bot. Food Qual., 85, 229–233.
Vaughan, S.P., Russell, K. (2004). Characterization of novel microsatellites and development of multiplex PCR for large-scale population studies in wild cherry, Prunus avium. Mol. Ecol. Notes, 4, 429–431. DOI: 10.1111/j.1471-8286.2004.00673.x
Wünsch, A. (2009). Cross-transferable polymorphic SSR loci in Prunus species. Sci. Hortic., 120, 348–352. DOI: 10.1016/j.scienta.2008.11.012
Josef Patzak
Hop Research Institute, Žatec, Czech Republic Research and Breeding
Hop Research Institute, Žatec, Czech Republic Research and Breeding
Alena Henychová
Hop Research Institute, Žatec, Czech Republic Research and Breeding
Hop Research Institute, Žatec, Czech Republic Research and Breeding
František Paprštein
Institute of Pomology, Holovousy, Czech Republic
Institute of Pomology, Holovousy, Czech Republic
Jiří Sedlák
Institute of Pomology, Holovousy, Czech Republic
Institute of Pomology, Holovousy, Czech Republic
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