EVALUATION OF GENETIC VARIABILITY WITHIN SWEET CHERRY (Prunus avium L.) GENETIC RESOURCES BY MOLECULAR SSR MARKERS
Josef Patzak
Hop Research Institute, Žatec, Czech Republic Research and BreedingAlena Henychová
Hop Research Institute, Žatec, Czech Republic Research and BreedingFrantišek Paprštein
Institute of Pomology, Holovousy, Czech RepublicJiří Sedlák
Institute of Pomology, Holovousy, Czech RepublicAbstract
Sweet cherry is a vegetatively propagated, perennial plant with high level of heterozygosity and ancient breeding history. Therefore, it is necessary to keep, conserve and evaluate known genetic resources for fu- ture breeding programs and fruit production stability. In present, the utilization of DNA molecular genetic analyses is the best suitable method for evaluation of individual accessions, thus we eliminated duplications and characterized the genetic relationships. In our work, we used PCR primer combinations for 19 SSR and 2 EST-SSR loci for analyses of 123 current, old and local sweet cherry cultivars from Czech genetic resources of Research and Breeding Institute of Pomology in Holovousy. In total, 115 polymorphic fragments were amplified, which we used for hierarchical cluster analysis of genetic variability. The result dendrograms were divided into three main clusters and ten subgroups. Clustering corresponded to genealogical and geobotanical characteristics of individual accessions as breeding history of several known accessions.
Keywords:
sweet cherry (Prunus avium L.), SSR and EST-SSR markers, genetic diversity, cultivars, hierarchical cluster analysisReferences
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Hop Research Institute, Žatec, Czech Republic Research and Breeding
Hop Research Institute, Žatec, Czech Republic Research and Breeding
Institute of Pomology, Holovousy, Czech Republic
Institute of Pomology, Holovousy, Czech Republic
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