Application of chosen Mus musculus mtDNA gene primers in amplification of Chinchilla lanigera parallel loci
Justyna Jarczak
Department of Animal Sciences, Institute of Genetics and Animal Breeding Polish Academy of Sciences, Jastrzębiec, Postępu 1, 05-552 MagdalenkaBrygida Ślaska
Department of Biological Bases of Animal Production, University of Life Sciences Akademicka 13, 20-950 LublinMagdalena Surdyka
Department of Biological Bases of Animal Production, University of Life Sciences Akademicka 13, 20-950 LublinAbstrakt
The purpose of the study was to evaluate the usefulness of primer sequences designed for amplification of mitochondrial genes of Mus musculus to analyze analogous loci in Chinchilla
lanigera's genome. After isolation of DNA from chinchilla's hair and its quantitative and qualitative estimation, the amplification of DNA by the PCR method was carried out. Fragments of 4 genes of mitochondrial genome were under consideration. Universal primers and primers designed on the basis of M. musculus genome were used for amplification of analogous loci of Ch. lanigera's genome. PCR products of 4 examined mtDNA sequences were received. Two of the four products showed homology of flank sequences of DNA individual fragments of both studied species belonging to Rodentia order. PCR optimization, including changes of polymerase, the concentrations of MgCl2 and primers as well as the melting temperature were determined for two of the four DNA sequences.
Słowa kluczowe:
Chinchilla lanigera, primer sequences, PCR, optimizationBibliografia
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Department of Animal Sciences, Institute of Genetics and Animal Breeding Polish Academy of Sciences, Jastrzębiec, Postępu 1, 05-552 Magdalenka
Department of Biological Bases of Animal Production, University of Life Sciences Akademicka 13, 20-950 Lublin
Department of Biological Bases of Animal Production, University of Life Sciences Akademicka 13, 20-950 Lublin
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Autor podpisuje oświadczenie o oryginalności dzieła i wkładzie poszczególnych osób.
