Abstract
The proteins from the family HSPB (small heat shock proteins) play a functional role in the regulation of intracellular processes (apoptosis, inflammatory response, chaperone activity concerning the protein folding and aggregation control) responsible for the protection from environmental stress factors. Mutations of genes encoding these proteins are the reason for neuronal cells dysfunctions, leading to myopathies, motor neuropathies and neurodegenerative disorders. The aim of the study was cytogenetic mapping of the HSPB genes in the pig genome with an application of FISH technique and the probes obtained from BAC clone containing sequences of HSPB1, HSPB2, CRYAB (alternative name HSPB5), HSPB6, HSPB8 genes, derived from the CHORI-242 Porcine BAC Library. Prior to in situ hybridization, carried out on metaphase chromosomes stained by DAPI bands technique, the presence of the studied genes in the selected clone was confirmed by means of PCR method with the use of the gene-specific primers. As a result of the experiments FISH signals in the chromosome regions SSC3p15 (HspB1), SSC9p21 (HspB2 and CRYAB), SSC6q12 (HspB6) and HspB8 SSC14q21 (HspB8) were obtained, which enabled to designate cytogenetic localization of the studied HSPB genes on the domestic pig genome map. The results obtained may help to elucidate the role of the HSPB genes in the pathomechanisms of myopathies and neuropathies in breeding animals
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