Skip to main navigation menu Skip to main content Skip to site footer

Vol. 29 No. 4 (2011)

Articles

Cytomolecular assay of size nucleolar organizer regions (NORs) polymorphism in Pietrain pigs

Submitted: March 31, 2021
Published: 2011-12-31

Abstract

The aim of this study was to identify and classify the size variants of nucleolar organizing regions (NORs) as markers useful for genetic characteristics of Pietrain breed pigs. On the
basis of cytomolecular analysis carried out by silver staining (Ag-I) and fluorescence in situ hybridization (FISH) techniques, four size variants of rDNA-FISH-signals and Ag-NORs silver
deposits were classified. Results of cytomolecular assay of size NORs polymorphism can be used in inter-breed or inter-species comparative surveys and studies of evolutionary relationships in Suidae family.

References

Danielak-Czech B., Kaczor U., Sharan M., 2009. Chromosome markers survey of Duroc pigs. Anim. Biol. 11, 237–241.
Danielak-Czech B., Słota E., Babicz M., Kozubska-Sobocińska A., Rejduch B., 2006. Ocena wielkości regionów jąderkotwórczych (NOR) u świń rasy puławskiej. Rocz. Nauk. Zoot. 33, 13–19.
Danielak-Czech B., Słota E., Lindblad K., Gustavsson I., 1999. Size polymorphism of nucleolar organizer regions in pigs bred in Poland as determined by FISH and silver staining technique.
Anim. Sci. Pap. Rep. 17, 163–171.
Harding R.M., Boyce A.J., Clegg J.B., 1992. The evolution of tandemly repetitive DNA: Recombination rules. Genetics 132, 847–859.
Lomholt B., Christenssen K., Hallenberg C., Frederiksen J., 1995. Porcine 5S rRNA genes mapped to 14q23 revealing syntenic relation to human HSPA6- and 7. Mamm. Genome 6, 439–441.
Mellink C.H.M., Bosma A.A., De Haan N.A., Wiegant J., 1991. Distribution of rRNA genes in breeds of domestic pig studied by non-radioactive in situ hybridization and selective silverstaining.
Genet. Sel. Evol. 23, suppl. 1, 168–172.
Mellink C.H.M., Bosma A.A., De Haan N.A., 1994. Variation in size of Ag-NORs and fluorescent rDNA in situ hybridization signals in six breeds of domestic pig. Hereditas 120 (2), 141–149.
Mellink C.H.M., Bosma A.A., De Haan N.A., Zijlstra C., 1996. Physical localization of 5S rRNA genes in the pig by fluorescence in situ hybridization. Hereditas 124, 95–97.
Miyake M.L., O’brien S.J., Kaneda Y., 1988. Regional localization of rDNA genes on pig chromosome 10 by in situ hybridization. Jap. J. Vet. Sci. 50, 341–345.
Pinkel D., Straume T., Gray J.W., 1986. Cytogenetic analysis using quantitative, high sensitive, fluorescence hybridization. Proceed. Nation. Acad. Sci. USA 83, 2934–2938.
Słota E., 1998. Polymorphism of pig chromosomes. Rocz. Nauk. Zoot. (Habilitation Thesis), 1–59.
Solinas Toldo S., Pieńkowska A., Fries R., Świtoński M., 1992. Localization of nucleolar organizer regions in farm animals by in situ hybridization method with a probe from a human rRNA gene. Proceedings of 10th European Colloquium on Cytogenetics of Domestic Animals, Utrecht, 228–231.
Świtoński M., Pietrzak A., 1992. Cytogenetic survey of AI boars; distribution of C-band and Ag-NORs polymorphism. Anim. Sci. Pap. Rep. 9, 91–96.
Świtoński M., Komisarek J., Pietrzak A., 1997a. The Polish Pig Genome Project: III. Chromosomal markers in generation F1. Anim. Sci. Pap. Rep. 15 (2), 93–99.
Świtoński M., Pietrzak A., Buczyński J., 1997b. Chromosomal markers (C-band and Ag-NOR) in the Zlotnicka Spotted pig. Anim. Sci. Pap. Rep. 15 (3), 173–178.
Wachtler F., Hopman A.H.N., Wiegant J., Schwarzacher H.G., 1986. On the position of nucleolus organizer regions (NORs) in interphase nuclei. Studies with a new, non-Autoradiographic in
situ Hybridization Method. Exp. Cell Res. 167, 227–240.

Downloads

Download data is not yet available.

Most read articles by the same author(s)

1 2 3 4 5 > >> 

Similar Articles

<< < 4 5 6 7 8 9 10 11 > >> 

You may also start an advanced similarity search for this article.