Skip to main navigation menu Skip to main content Skip to site footer

Vol. 29 No. 4 (2011)

Articles

Differentiation of Candida species and Candida rugosa strains with the use of molecular markers in healthy horses

Submitted: April 1, 2021
Published: 2011-12-31

Abstract

The aim of the study was identification of molecular markers useful in differentiating the species of selected yeast-like fungi on the skin and mucous membranes of horses and in identification of Candida rugosa strains. The study material was smears from horse skin and mucous membranes. The isolates were assayed with the use of API 20C AUX (bioMérieux) biochemical tests. The examinations involved C. lusitaniae, C. parapsilosis and C. rugosa. RAPD-PCR analyses included amplification with the use of 20 arbitrary primers (OPG01-OPG20). Genetic distances between the Candida rugosa strains were estimated by the Popgen32 programme. The RAPD-PCR analysis yielded amplification products of all the markers used in the study. The number of speciesspecific DNA fragments ranged from 0 to 6, depending on the primer. At least two specific DNA fragments were found for each of the studied species in the case of primers OPG04, OPG05, OPG06, OPG09, OPG11 and OPG14, which may indicate the possibility of their use for species identification. The present work is the first attempt to describe genetic differences between Candida rugosa strains sampled from healthy Hucul horses. Four genotypes within the Candida rugosa species, whose frequency ranged from 0.06 to 0.62, were obtained upon combination of DNA profiles of three primers (OPG04, OPG05 and OPG11). The genetic distances identified indicated considerable diversity of the studied strains. The genetic diversity may corroborate different environmental epidemiologic sources of the strains and may be related to their non-pathogenic nature.

References

Baires-Varguez L., Cruz-García A., Villa-Tanaka L., Sánchez-García S., Gaitán-Cepeda L.A., Sánchez-Vargas L.O., Quindós G., Hernández-Rodríguez C., 2007. Comparison of a randomly
amplified polymorphic DNA (RAPD) analysis and ATB ID 32C system for identification of clinical isolates of different Candida species. Rev. Iberoam Micol. 24, 148–151.
Banerjee U., 2005. Progress in diagnosis of opportunistic infections in HIV/AIDS. Indian J. Med. Res. 121, 395–406.
Bautista-Muñoz C., Boldo X.M., Villa-Tanaca L., Hernández-Rodríguez C., 2003. Identification of Candida spp. by randomly amplified polymorphic DNA analysis and differentiation between Candida albicans and Candida dubliniensis by direct PCR methods. J. Clin. Microbiol. 41, 414–420.
Chang H.C., Leaw S.N., Huang A.H., Wu T.L., Chang T.C., 2001. Rapid identification of yeasts in positive blood cultures by a multiplex PCR method. J. Clin. Microbiol. 39, 3466–3471.
Colombo, A.L., Melo A.S.A., Rosas R.F.C., Salomao R., Briones M., Hollis R.J., Messer S.A., Pfaller M.A., 2003. Outbreak of Candida rugosa candidemia: an emerging pathogen that may
be refractory to amphotericin B therapy. Diagn. Microbiol. Infect. Dis. 46: 253–257.
Krukowski H., Lisowski A., Rózański P., Skórka A., 2006. Yeasts and algae isolated from cows with mastitis in the Southeastern part of Poland. Pol. J. Vet. Sci. 9,181–4.
Lockhart S.R., Joly S., Pujol C., Sobel J.D., Pfaller M.A., Soll D.R., 1997. Development and verification of fingerprinting probes for Candida glabrata. Microbiology 143, 3733–3746.
Saitou N., Nei M., 1987. The Neighbor-Joining Method – a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406–425.
Santos R.C., Marin J.M., 2005. Isolation of Candida spp. from mastitic bovine milk in Brazil. Mycopathologia 159, 251–3.
Saville S.P., Thomas D.P., López-Ribot J.L., 2005. Use of genome information for the study of the pathogenesis of fungal infections and the development of diagnostic tools. Rev. Iberoam Micol. 22, 238–241.
Seker E., 2010. Identification of Candida species isolated from bovine mastitic milk and their in vitro hemolytic activity in western Turkey. Mycopathologia: 169, 303–308
Steffan P., Vázquez J.A., Boikov D., Xu C., Sobel J.D., Akins R.A., 1997. Identification of Candida species by randomly amplified polymorphic DNA fingerprinting of colony lysates.
J. Clin. Microbiol. 35, 2031–2039.
Taylor, J. W., Geiser D. M., Burt A., Koufopanou V., 1999. The evolutionary biology and population genetics underlying fungal strains typing. Clin. Microbiol. Rev. 12, 126–146.
Tamura K., Dudley J., Nei M., Kumar S., 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24, 1596–1599.
Yeh F.C., Yang R.C., 2000. PopGen32 computer program microsoft windows based freeware for population genetic analysis. Http://www.ualberta.ca/_fyeh/index.htm

Downloads

Download data is not yet available.

Most read articles by the same author(s)

Similar Articles

<< < 1 2 3 4 5 6 7 > >> 

You may also start an advanced similarity search for this article.