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Tom 79 Nr 2 (2024)

Artykuły

Effect of preparations containing humic substances and pure humic acids on colony growth and spore germination of entomopathogenic fungi from the Beauveria genus

DOI: https://doi.org/10.24326/as.2024.5301
Przesłane: 20 listopada 2023
Opublikowane: 02-12-2024

Abstrakt

W doświadczeniu zbadano wpływ dostępnych na rynku preparatów zawierających substancje humusowe w porównaniu z czystymi kwasami humusowymi na wzrost i kiełkowanie zarodników grzybów entomopatogenicznych z rodzaju Beauveria           w warunkach in vitro. Preparaty AmiAGRA, HumiAGRA, AlgoHUM (zalecana dawka polowa) oraz czyste kwasy humusowe, które zostały wyekstrahowane z torfu, węgla brunatnego oraz podłoża popieczarkowego dodano do podłoża hodowlanego Sabourauda. Obserwację wzrostu kolonii testowanych gatunków grzybów owadobójczych prowadzono co 5 dni, aż do dnia 20 mierząc ich średnicę (mm). W drugim etapie doświadczenia, podłoże hodowlane z dodatkiem preparatów i czystych kwasów humusowych nanoszono cienką warstwą na powierzchnię szkiełek podstawowych i wprowadzono 0,1 ml wodnego roztworu z zarodnikami. Obserwację prowadzono po 24 i 48 godzinach, a uzyskane wyniki wyrażono procentowo w stosunku do kontroli. Przeprowadzone badania wykazały, że w 20 dniu hodowli (średnio) preparaty zawierające substancje humusowe działały stymulująco, natomiast czyste kwasy nieznacznie ograniczały wzrost kolonii testowanych izolatów. Wzrost kolonii grzyba B. basianna najsilniej stymulował preparat AmiAGRA, a B. brongniartii HumiAGRA. Udział skiełkowanych zarodników testowanych izolatów, po 48 godzinach kontaktu z podłożem był większy niż po 24. Stwierdzono iż, więcej zarodników kiełkowało na podłożach z dodatkiem preparatów zawierających substancje humusowe, niż czyste kwasy humusowe.                         

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