Abstrakt
Rdza brunatna powodowana przez grzyb Puccinia triticina oraz mączniaka prawdziwego Blumeria graminis są jednymi z najgroźniejszych chorób pszenicy zwyczajnej (Triticum aestivum L.). Najskuteczniejszą metodą kontrolowania i ograniczania skutków wywołanych przez te patogeny jest wprowadzanie do uprawy odmian charakteryzujących się zwiększoną odpornością. Celem prezentowanych badań była identyfikacja genów odporności na rdzę brunatną – Lr21 oraz mączniaka prawdziwego – Pm4b w polskich odmianach pszenicy zwyczajnej oraz opracowanie warunków multiplex PCR w celu jednoczesnej identyfikacji tych genów. Przedmiotem badań było 30 polskich odmian pszenicy zwyczajnej. Wyizolowane DNA zostało poddane jednoczesnej amplifikacji ze starterami specyficznymi dla obu analizowanych genów. Wykazano, że opracowane warunki multiplex PCR umożliwiają jednoczesną identyfikację genów Lr21 i Pm4b. Multiplex PCR może być wykorzystany w hodowli odpornościowej pszenicy zwyczajnej wspomaganej markerami do identyfikacji tych genów.
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