Abstract
The purpose of the study was to determine which fungal species colonize the shoots of apple, pear, cherry, plum, apricot and peach in south-eastern Poland and whether Diaporthe (Phomopsis) isolates there are among them. The study was conducted in 2010–2012 and the plant material was collected from five orchards.
In three of these chemical controls were carried out, while there was no protection applied in the other two. The results showed that species composition of the fungi was very similar regardless of host plant and year of study. Alternaria spp., Fusarium spp. and Phoma spp. were isolated most frequently from the studied shoots. Fungi cultures known for pathogenicity towards fruit trees such as Botrytis cinerea, Colletotrichum gloeosporioides, Leucostoma spp., Monilinia spp. and Neofabraea spp. were also isolated. Moreover, there were isolates of Diaporthe (Phomopsis) among the fungi present in the shoots. Cultures of these fungi were obtained both from shoots with disease symptoms and from visually healthy ones but mainly from shoots originating from unprotected orchards, what indicates their greater threat to fruit trees grown without chemical protection. This is the first documented report of the occurrence of Diaporthe (Phomopsis) on fruit trees in Polish conditions.
References
Adams, G.C., Surve-Iyver, R.S., Iezzoni, A.F. (2002). Ribosomal DNA sequence divergence and group I introns within the Leucostoma species L. cinctum, L. persoonii, and L. parapersoonii sp. nov., ascomycetes that cause Cytospora canker of fruit trees. Mycologia, 94(6), 947–967.
Arsenijevič, M., Gavrilovič V. (2005). Phomopsis perniciosa Grove – uzročnik truleži uskladištenih plodova jabuke. Pestic. Fitomed., 20(3), 189–194.
Castillo-Pando, M.S., Nair, N.G., Emmett, R.W., Wicks, T.J. (1997). Inhibition in pycnidial viability of Phomopsis viticola on canes in situ as an aid to reducing inoculum potential of cane and leaf blight disease of grapevines. Austral. Plant Pathol., 26, 21–25.
Danielewicz, B., Gwiazdowski, R., Bednarek-Bartsch, A. (2013). Wpływ wybranych fungicydów na ograniczanie wzrostu kultur rodzaju Fusarium. Prog. Plant Prot. / Post. Ochr. Roślin, 53, 4, 759–61.
Farr, D.F., Castlebury, L.A., Pardo-Schultheiss, R.A. (1999). Phomopsis amygdali causes peach shoot blight of cultivated peach trees in the southeastern United States. Mycologia, 91, 1008–1015.
Farr, D.F., Castlebury, L.A., Rossman, A.Y. (2002). Morphological and molecular characterization of Phomopsis vaccinii and additional isolates of Phomopsis from blueberry and cranberry in the eastern United States. Mycologia, 94, 494–504.
Frużyńska-Jóźwiak, D., Andrzejczak, R. (2010). Wpływ wybranych preparatów na zdrowotność ozdobnych drzew i krzewów iglastych. Prog. Plant Prot./ Post. Ochr. Roślin, 50(4), 2067–2071.
Gao, L.L., Hang, Q., Sun, X.Y., Jiang, L., Hang, R., Sun, G.Y., Zha, Y.L., Bigos, A.R. (2013). Etiology of moldy core, core browning and core rot of Fuji apple in China. Plant Dis., 97, 4, 510–516.
Grabowski, M. (1993). Study on occurrence of Nectria galligena mycelium in wood of affected shoots of apple-trees at various points situated above and below the place of infection. Phytopathol. Pol., 5, 23–29.
Grabowski, M. (1996). Grzyby kolonizujące zrakowacenia na pędach jabłoni w sadach chemicznie chronionych oraz ich wpływ na rozwój procesu chorobowego wy-wołanego przez Nectria galligena Bres. Acta Agrar. Silv., Ser. Agrar., 34, 35–41.
Grabowski, M. (2002). Badania nad grzybami zasiedlającymi rozdrobnione pędy jabłoni pozostawione w sadzie po cięciu. Acta Agrobot., 55, 1, 79–87.
Grabowski, M., Leś, B. (1996). Fungi inhabiting cancerations on apple shoots and their application against Nectria galligena Bres. Phytopathol. Pol., 11, 15–22.
Gramaje, D., Agustí-Brisach, C., Pérez-Sierra, A., Moralejo, E., Olmo, D., Mostert, L., Damm, U., Armengol, J.(2012). Fungal trunk pathogens associated with wood decay of almond trees on Mallorca (Spain). Persoonia, 28, 1–13.
Gomes, R.R., Glienke, C., Videira, S.I.R., Lombard, L., Groenewald, J.Z., Crous, P.W. (2013). Diaporthe: a genus of endophitic, saprobic and plant pathogenic fungi. Persoonia, 31, 1–41.
Halleen, F., Crous, P.W., Petrini, O. (2003). Fungi associated with healthy grapevine cuttings in nurseries, with special reference to pathogens involved in the decline of young vines. Australas. Plant Pathol., 32, 47–52.
Kačergius, A., Gabler, J., Jovaišienè, Z. (2004). Determi-nation of Phomopsis canker and dieback of highbush blueberries and cranberries in Lithuania. Agron. Vēstis/ Latv. J. Agron. 7, 71–78.
Kanematsu, S., Minaka, N., Kobayashi, T., Kudo, A., Ohtsu, Y. (2000). Molecular phylogenetic analysis of ribosomal DNA internal transcribed spacer regions and comparison of fertility in Phomopsis isolates from fruit trees. J. Gen. Plant Pathol., 66, 191–201.
Karaoglanidis, G.S., Bardas, G. (2006). First report of Phomopsis fruit decay on apple caused by Phomopsis mali in Greece. Plant Dis., 90(3), 375.
Kawchuk, L.M., Hutchison, L.J., Verhaeghe, C.A., Lynch, D.R., Bains, P.S., Holey, J.D. (2002). Isolation of β-tubulin gene and characterization of thiabendazole resistance in Giberella pulicaris. Can. J. Plant Pathol., 24(2), 233–238.
Kiecana, I., Mielniczuk, E. (2010). Fusarium head blight of winter rye. Acta Agrobot., 63(1), 129–135.
Kiecana, I., Cegiełko, M., Mielniczuk, E., Perkowski, J. (2012). The occurrence of Fusarium poae (Peck) Wollenw. on oat (Avena sativa L.) panicles and its harmfulness. Acta Agrobot., 65(4), 169–178.
Kirk, P.M., Cannon, P.M., Minter, D.W., Stalpens, J.A. (2008). Ainsworth & Bisby’s Dictionary of Fungi. 10th ed. CAB International, Wallingford, UK, 640 pp.
Król, E. (2002). Determination of genetic variability within Phomosis spp. using RAPD method. Phytopathol. Pol., 25, 35–46.
Król, E. (2005). Identification and differentiation of Phomopsis spp. isolates from grapevine and some other plant species. Phytopathol. Pol., 35, 151–156.
Król, E. (2006). Grzyby zasiedlające zdrowe łozy winorośli (Vitis spp.) w wybranych szkółkach. Acta Agrobot., 59(2), 163–173.
Król, E. (2007). Phomopsis viticola Sacc. jako patogen winorośli na świecie i w Polsce. Post. Nauk Roln., 4, 85–96.
Król, E., Kowalik, B. (2010). Charakterystyka grzybów rodzaju Phomopsis wyizolowanych z roślin sadowniczych. Prog. Plant Prot./ Post. Ochr. Roślin, 554, 53–59.
Król, E., Kowalik, B. (2011). Grzyby z rodzaju Phomopsis występujące na pędach roślin sadowniczych. Post. Nauk Rol., 2, 31–42.
Latorre, B.A., Rioja, M.E., Lillo, C., Muñoz, M. (2002). The effect of temperature and wetness duration on infection and a warning system for European canker (Nectria galligena) of apple in Chile. Crop Prot., 21(4), 285–291.
Machowicz-Stefaniak, Z. (1993). Phomopsis viticola Sacc. (Sphaeropsidales Deuteromycotina) nowy w Polsce patogen pędów winorośli. Acta Mycol., 28, 157–160.
Michailides, T.J., Thomidis, T. (2006). First report of Phomopsis amygdali causing fruit rot on peaches in Greece. Plant Dis., 90(12), 1551.
Mirabolfathy, M., Hoseinian, L., and Mirhoseini Moghadam, A. (2013). First report of Phomopsis amygdali (Del.) Tuset & Portilla causing galls on common hazel (Corylus avellana) twigs in Iran. Iran. J. Plant Pathol., 49(1), 43–45.
Mostert, L., Denman, S., Crous, P.W. (2000). In vitro screening of fungicides against Phomopsis viticola and Diaporthe perjuncta. S. Afr. J. Enol. Vitic., 21, 62–65.
Mostert, L, Crous, P.W., Kang, C.J., Phillips, A.J.L. (2001). Species of Phomopsis and a Libertella sp. occurring on grapevines with specific reference to South Africa: morphological, cultural, molecular and pathological characterization. Mycologia, 93, 145–166.
Osowski, J. (2004). Skuteczność różnych fungicydów zawierających mankozeb w ograniczaniu rozwoju al-ternariozy ziemniaka w badaniach polowych i laboratoryjnych. Biul. Inst. Hod. Aklimat. Rośl., 233, 295–302.
Pres, N.A., Timmer, L.W. (2006). Evaluation of the Alter-Rater model for spray timing for control of Alternaria brown spot on Murcott tangor in Brazil. Crop Prot., 25, 454–460.
Regner, K.M., Johnson, D.A., Gross, D.C. (1990). Etiology of canker and dieback of sweet cherry trees in Washington State. Plant Dis., 74, 430–433.
Romanazzi, G., Mancini, V., Murolo, S. (2012). First report of Leucostoma cinctum on sweet cherry and European plum in Italy. Phytopathol. Mediterr., 51(2), 365−368.
Rossman, A.Y., Udayanga, D., Castlebury, L.A., Hyde, K.D. (2014). Proposal to conserve the name Diaporthe eres, with a conserved type, against all other competing names (Ascomycota, Diaporthales, Diaporthaceae). Taxon 63, 934–935.
Stuart, M.R., Bastianel, F.A.A., Machado, M.A. (2009). Alternaria brown spot. Laranja, 30, 1–2, 29–44.
Spotts, R.A., Facteau, T.J., Cervantes, L.A., Chestnut, N.E. (1990). Incidence and control of Cytospora canker and bacterial canker in a young sweet cherry orchard in Or-egon. Plant Dis., 74, 577–580.
Szmagara, M. (2009). Biodiversity of fungi inhabiting the highbush blueberry stems. Acta Sci. Pol. Hortorum Cultus, 8(1), 37–50.
Szmagara, M., Machowicz-Stefaniak, Z. (2005). Grzyby porażające pędy borówki wysokiej (Vaccinium corymbosum L.). Prog. Plant Prot./ Post. Ochr. Roślin, 45(2), 1130–1133.
Timmer, L.W., Zitko, S.E. (1997). Evaluation of fungicides for control of Alternaria brown spot and citrus scab. Proc. Fla. State Hort. Soc., 110, 71–76.
Tylkowska, K., Grabarkiewicz-Szczesna, J., Iwanowska, H. (2003). Production of toxins by Alternaria alternata and A. radicina and their effects on germination of car-rot seeds. Seed Sci. Technol., 31, 309–316.
Udayanga, D., Liu, X.X., McKenzie, E.H.C., Chukeatirote, E., Bahkali, A.H., Hyde, K.D. (2011). The genus Phomopsis: biology, applications, species concepts and names of common phytopathogens. Fungal Divers., 50, 189–225.
Udayanga, D., Castlebury, L.A., Rossman, A. Y., Chukeatirote, E., Hyde, K.D. (2014). Insights into the genus Diaporthe: phylogenetic species delimitation in the D. eres species complex. Fungal Divers., 67, 203–229.
Uddin, W., Stevenson, K.L., Pardo-Schultheiss, R.A. (1997). Pathogenicity of a species of Phomopsis caus-ing a shoot blight on peach in Georgia and evaluation of possible infection courts. Plant Dis., 81, 983–989.
Uddin, W., Stevenson, K.L., Pardo-Schultheiss, R.A., Rehner, S.A. (1998). Pathogenic and molecular characterization of three Phomopsis isolates from peach, plum and Asian pear. Plant Dis., 82, 732–737.
Valiuškaitê, A., Raudonis, L. (2008). Epidemiology of bark diseases of apple tree in Lithuania. Sodinink. Daržinink., 27(4), 51–57.
Walkowiak, W., Krzyśko-Łupicka, T. (2014). Nowe rozwiązania w ochronie zbóż przed fuzariozami. Prog. Plant Prot./Post. Ochr. Rośl., 54(2), 127–134.
Wenneker, M., Pham, K.T.K., Lemmers, M.E.C., de Boer, F.A., van der Lans, A.M., van Leeuwen, P.J., Hollinger, T.C. (2016). First report of Fusarium avenaceum causing wet care rot of “Elstar” apples in the Netherlands. Plant Dis., 100(7), 1501.
Živković, S.T., Stojanović, S.D., Balaź, J., Gavrilović, V.P. (2007). Characteristics of Phomopsis sp. isolates of plum trees origin. Proc. Nat. Sci., Matica Srpska Novi Sad, 113, 83–91.
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