Skip to main navigation menu Skip to main content Skip to site footer

Vol. 15 No. 6 (2016)

Articles

IDENTIFICATION OF NON-PATHOGENIC FUNGI OF RICE AND THE EVALUATION OF THEIR EFFECT ON BIOLOGICAL CONTROL OF Pyricularia grisea, THE CAUSAL AGENT OF RICE BLAST DISEASE in vitro

Submitted: November 3, 2020
Published: 2016-12-31

Abstract

Rice blast disease, caused by Pyricularia grisea, is an important rice disease in Iran and throughout the world. Out of 150 infected samples collected from paddy fields of Guilan province, 57 fungal isolates were isolated for which PDA and W.A media were used. Morphological characteristics were used for identification of these fungi. It was found out that the isolated fungi were belonged to Pyricularia grisea, Ulocladium alternariae, Ulocladium cf. alternariae, Ulocladium cf. consortiale, Ulocladium sp., Curvularia pallescens, Preussia sp., Epicoccum sp. and Trichoderma harzianum. According to pathogenicity tests, all isolates of P. grisea were proved to be pathogenic on rice and fungal isolates belonging to other fungal genera which did not cause disease on rice were selected for biological control studies and to do so, were used different methods. Results showed that eight isolates of T. harzianum, U. cf. consortiale, Ulocladium sp., Epicoccum sp., Preussia sp., C. pallescens, U. alternariae and U. cf. alternariae, had the highest in-hibitory effect in mycelial growth of P. grisea, respectively. In hyperparasitism test, hyphae of T. harzianum did not coil around the mycelium of P. grisea. In these experiments, T. harzianum and U. cf. consortiale isolates had the highest inhibitory effect on growth rate of P. grisea and was known as the most effective isolates. Based on results, volatile metabolites method was best method in control of P. grisea in vitro.

References

Abdel-Fattah, G.M., Shabana, Y.M., Ismail, A.E., Rashad, Y.M. (2007). Trichoderma harzianum: a biocontrol agent against Bipolaris oryzae. Mycopathologia, 164(2), 81–89.
Amini, E., Tajik Ghanbari, M.A., Hasanzadeh, N. (2012). Biological control of rice blast disease by Streptomyces erythraea in vitro and greenhouse conditions. Proceeding of 6th National Conference New Ideas in Agriculture, 29–30 February, Khvorasgan, Iran, 234–238.
Arenal, F., Platas, G., Peláez, F. (2004). Variability of spore length in some species of the genus Preussia (Sporormiella). Mycotaxon, 89, 137–151.
Arenal, F, Platas, G, Pelaez, F (2007). A new endophytic species of Preussia (Sporomiaceae) inferred from morphological observations and molecular phylogenetic analysis. Fung. Di-vers., 25,1–17.
Boff, P., Köhl, J., Gerlagh, M., de Kraker, J. (2002). Biocontrol of grey mould by Ulocladium atrum applied at different flower and fruit stages of strawberry. BioContr., 47(2), 193–206.
Cain, R.F. (1961). Studies of coprophilous ascomycetes. VII. Preussia. Can. J. Bot., 39, 1633–1666.
De Figueirêdo, G.S., De Figueirêdo, L.C., Cavalcanti, F.C.N., Dos Santos, A.C., Da Costa, A.F., De Oliveira, N.T. (2010). Biological and chemical control of Sclerotinia sclerotiorum using Trichoderma spp. and Ulocladium atrum and pathogenicity to bean plants. Braz. Arch. Biol. Tech., 53(1), 1–9.
Dennis, C., Webster, J. (1971 a). Antagonistic properties of species-groups of Trichoderma. I. Production of nonvolatile antibiotics. T. Brit. Mycol. Soc., 57(1), 25–39.
Dennis, C., Webster, J. (1971 b). Antagonistic properties of species-groups of Trichoderma. II. Production of volatile antibiotics. T. Brit. Mycol. Soc., 57(1), 41–48.
Ellis, M.B. (1971). Dematiaceous Hyphomycetes. CMI, Kew, England.
Gams, W., Bissett, J. (1998). Morphology and identification of Trichoderma. In: Trichoderma and Gliocladium. Vol. 1. Basic biology, taxonomy and genetics, Kubicek, C.P., Harman, G.E. (eds)., Taylor and Francis Ltd., London, 3–34.
Gouramanis, G. (1997). Biological and chemical control of rice blast disease (Pyricularia oryzae) in Northern Greece. Cah. Options Méditter., 15(3), 61–68.
Hajano, J.U.D., Lodhi, A.M., Pathan, M.A., Khanzada, M.A., Serwar Shah, G. (2012). In vitro evaluation of fungicides, plant extracts and biocontrol agents against rice blast pathogen Magnaporthe oryzae. Pakistan J. Bot., 44, 1775–1778.
IRRI (1989). Biological control; Blast control, International Rice Research Institute (IRRI), Annual Report for 1988. The Philippines, 231–232.
Jaiganesh, V., Eswaran, A., Balabaskar, P., Kannan, C. (2007). Antagonistic activity of Serratia marcescens against Pyricularia oryzae. Not. Bot. Hort. Agrobot. Cluj-Na., 35(2), 48–54.
Javadi, L., Naeimi, Sh., Rezaee, S., Khosrav, V. (2014). Biological control of rice blast disease with native Trichoderma isolates in Mazandaran province. Biocont. Plant Protect., 2(1), 1–15.
Javan Nikkhah, M. (2001). Study on the genetic diversity of Magnaporthe grisea, rice blast pathogen by using molecular, pathogenicity and vegetative compatibility characters in Guilan province, Iran. Ph.D thesis, University of Tehran, Karaj.
Karthikeyan, V., Gnanamanickam, S.S. (2008). Biological control of Setaria blast (Magnaporthe grisea) with bacterial strains. Crop Prot., 27(2), 263–267.
Khalili, E., Sadravi, M., Naeimi, Sh., Khosravi, V. (2012). Biological control of rice brown spot with native isolates of three Trichoderma species. Braz. J. Microb., 43(1), 297–305.
Khodaparast, S.A., Sahragard, A. (2004). Rice diseases. The University of Guilan Press.
Khosravi, V., Naeimi, Sh., Rostami, M., Omrani, M., Bahramei, M. (2011). Evaluation of three commercial biological products on the rice blast disease in Mazandaran. Proceeding of 19th Iranian Plant Protection Congress, 31 July–3 August, Tehran, Iran, 828.
Mohammadian, S. (2013). Biological control of Bipolaris spp., the causal agent of brown spot disease of rice by some antagonistic fungi in Guilan province. Master’s thesis, Islamic Azad University, Sci. and Res. Branch, Guilan, Rasht.
Naeimi, Sh., Okhovvat, S.M., Javan-Nikkhah, M., Vágvölgyi, C., Khosravi, V., Kredicks, L. (2011). Biological control of Rhizoctonia solani AG1-1A, the causal agent of rice sheath blight with Trichoderma strains. Phytopathol. Meditterr., 49(3), 287–300.
Ningthoujam, D.S., Sanasam, S., Tamreihao, K., Nimaichand, S. (2009). Antagonistic activities of local actinomycetes isolates against rice fungal pathogen. Afr. J. Microbiol. Res., 3(11), 737–742.
Ou, S.H. (1985). Rice diseases. Commonwealth Mycological Institute, 2nd ed.
Ouazzani Touhami, A., Mouria, A., Douira, A., Benkirane, R., Mlaik, A., El-Yachioui, M. (1998). In vitro effect of pH and temperature on the ability of Trichoderma spp. to reduce the growth of Pyricularia oryzae. Al Awania, 96, 19–24.
Padasht Dehkaei, F. (2001). The final report identifying biological factors controlling rice blast disease. Rice Research Institute of Iran.
Padasht Dehkaei, F., Izadyar, M. (2007). Study on the biological control of rice blast disease in the field conditions. J. Agri. Sci. Nat. Res., 13, 84–92.
Rostami, M., Momeni, A., Khosravi, V., Zare, L., Darvishzadeh, N., Omrani, M., Ghalandari, M. (2012). Identification and investigation of effects of antagonistic bacterial agents on rice blast control. Proceeding of 20th Iranian Plant Protection Congress, 25–29 August. Shiraz, Iran, 221–225.
Safari Motlagh, M.R., Padasht Dehkaee, F., Hedjaroud, G.A. (2005). Rice brown spot disease and evaluation of the response of some rice cultivars to it. J. Sci. Tech. Agri. Nat. Res., 9(2), 171–182.
Safari Motlagh, M.R. (2010). Isolation and characterization of some important fungi from Echi-nochloa spp., the potential agents to control rice weeds. Aust. J. Crop Sci., 4(6), 457–460.
Sena, A.P., Chaibub, A.A., Côrtes, M.V., Silva, G.B., Silva-Lobo, V.L., Prabhu, A.S., Filippi, M.C.C., Araújo, L.G. (2013). Increased enzymatic activity in rice leaf blast suppression by crude extract of Epicoccum sp. Trop. Plant Pathol., 38(5), 387–397.
Simmons, E.G. (1967). Typification of Alternaria, Stemphylium, and Ulocladium. Mycologia, 59(1), 67–92.
Sivakumar, D., Wilson Wijeratnam, R.S., Wijesundera, R.L.C., Marikar, F.M.T., Abeyesekere, M. (2000). Antagonistic effect of Trichoderma harzianum on postharvest pathogens of Ram-butan (Nephelium lappaceum). Phytoparasitica, 28(3), 240–247.
Sivanesan, A. (1987). Gramainicolous species of Bipolaris, Curvularia, Drechslera, Exserohilum and their telemorphs. CAB International Mycological Institute.
Sutton, D.A., Fothergill, A.W., Rinaldi, M.G. (1998). Guide to clinically significant fungi (1th ed.).Williams & Wilkins Publish., Baltimore.

Downloads

Download data is not yet available.

Similar Articles

<< < 8 9 10 11 12 13 14 15 16 17 > >> 

You may also start an advanced similarity search for this article.