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Vol. 12 No. 2 (2013)

Articles

BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF THE POLISH Zucchini yellow mosaic virus ISOLATES

Submitted: December 7, 2020
Published: 2013-04-30

Abstract

The diversity of Zucchini yellow mosaic virus (ZYMV) isolates from cucumber and zucchini plants growing in different regions of Poland was analyzed using biological
tests and molecular biology techniques. The isolates differed in their host range and symptoms induced by them on a series of plant species. In addition, the analysis of the genetic diversity of the coat protein (CP) gene revealed high level of nucleotide variability among the isolates. Comparison of the CP gene sequences of 70 isolates from different geographical regions worldwide showed that the Polish isolates belong to different groups and they do not form a monophyletic cluster with European isolates. Interestingly, among the central European ZYMV isolates lower variability has been observed previously. The ratio of nonsynonymous to synonymous polymorphic sites showed a dominant negative selection however codons which might undergo positive selection were also identified. Moreover, the evidences for recombination in analyzed sequences of the CP gene of the analyzed ZYMV isolates were provided.

References

Berger P.H., Wyatt S.D., Shiel P.J., Silbernagel M.J., Druffel K., Mink G.I., 1997. Phylogenetic analysis of the Potyviridae with emphasis on legume-infecting potyviruse. Arch. Virol. 142, 1979–1999.
Chen J., Chen J., Adams M.J., 2001. A universal PCR primer to detect members of the Potyviridae and its use to examine the taxonomic status of several members of the family. Arch. Virol. 146, 757–766.
Clark M.F., Adams A.N., 1977. Characterization of the microplate method of enzyme-linked immunosorbent assay for detection of plant viruses. J. Gen.Virol. 34, 475–483.
Domier L.L., Shaw J.G., Rhoads R.E., 1987. Potyviral proteins share amino acid sequence homology with picorna-, como- , and caulimoviral proteins. Virology 158, 20–27.
Glasa M., Svoboda J., Novakova S., 2007. Analysis of the molecular and biological variability of Zucchini yellow mosaic virus isolates from Slovakia and Czech Republic. Virus Genes 35, 415–421.
Kosakovsky Pond S.L., Frost S.D.W., 2005. DATAMONKEY: rapid detection of selective pressure on individual sites of codon alignments. Bioinformatics 21, 2531–2533.
Kosakovsky Pond S.L., Frost S.D.W., Muse S.V., 2004. HyPhy: hypothesis testing using phylogenies. Bioinformatics 21, 676–679.
Martin D.P., Lemey P., Lott M., Moulton V., Posada D., Lefeuvre P., 2010. RDP3: a flexible and fast computer program for analyzing recombination. Bioinformatics 26, 2462–2463.
Pfosser M.F., Baumann H., 2002. Phylogeny and geographical differentiation of zucchini yellow mosaic virus isolates (Potyviridae) based on molecular analysis of the coat protein and part of the cytoplasmic inclusion protein genes. Arch. Virol. 147, 1599–1609.
Pospieszny H., Cajza M., Plewa R., 2003. First Report of Zucchini yellow mosaic virus in Cucumber in Poland. Plant Dis. 87 (11), 1399.
Pospieszny H., Hasiów B., Borodynko N., 2007. A Polish Isolate of Zucchini yellow mosaic virus from Zucchini is Distinct from Other European Isolates. Plant Dis. 91, 639.
Revers F., Le Gall O., Candresse T., Maule A.J., 1999. New advances in understanding the molecular biology of plant-potyvirus interactions. Mol. Plant-Microbe Interact. 12, 367–376.
Schrijnwerkers C., Huijberts N., Bos L., Neth L., 1991. Zucchini yellow mosaic virus; two outbreaks in the Netherlands and seed transmissibility. J. Plant Pathol. 97, 187–191.
Shukla D.D., Frenkel M.J., Ward C.W., 1991. Structure and function of the potyvirus genom with special reference to the coat protein coding region. Can. J. Plant Pathol. 97, 187–191.
Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S., 2011. MEGA5: molecular evolutionary genetics using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28 (10), 2731–2739.
Thompson D., Higgins D.G., Gibson T.J., 1994. CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acids Res. 22, 4673–4680.
Urcuqui-Inchima S., Haenni A., Bernardi F., 2001. Potyvirus proteins: a wealth of functions. Virus Res. 74, 157–175.

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