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The comparison of antioxidant capacity and HPTLC polyphenolic profile of green fruits and leaves of walnut (Juglans regia L.) cultivars grown in Poland

DOI: https://doi.org/10.24326/asphc.2025.5589
Submitted: 28 August 2025
Published: 08.12.2025

Abstract

Walnut (Juglans regia L.) has a wide range of applications in food, pharmaceutical and cosmetics industries, however both unripe fruits and leaves have not been widely evaluated for varietal variation so far. Due to, leaves and immature fruits of seven walnut cultivars (Lake, Mars, Franquette, U02, SK04/10, Broadview, and Resovia) grown in Poland were compared for the first time in terms of antioxidant capacity (DPPH and FRAP), total phenolic content and qualitative polyphenol profile (HPTLC). The highest phenolic content was found for the Franquette (p < 0.05), both for leaves and nuts. In general, lower variability was observed for leaves, in the case of green nuts the results were more diversified by cultivar. The rapid and cost-saving HPTLC method was used to compare the polyphenolic profiles of the extracts. Cultivar-dependent differences in phenolic acids (mainly syringic) and flavonoids (mainly derivatives of quercetin) presence were found. Despite of observed differences between cultivars, it was found that both part of walnut are abundant in polyphenols. However it has been confirmed that the selection of a phytochemically appropriate cultivar may be important in providing a particularly valuable raw material for the food, pharmaceutical and cosmetic applications, thus the further quantitative studies are required.

References

  1. Amaral, J.S., Seabra, R.M., Andrade, P.B., Valentão, P., Pereira, J.A., Ferreres, F. (2004). Phenolic profile in the quality control of walnut (Juglans regia L.) leaves. Food Chem., 88(3), 373–379. https://doi.org/10.1016/j.foodchem.2004.01.055
  2. Balapanov, I., Artykhova, L. (2021). Management of walnut genetic resources to improve the efficiency of the breeding process in the South of Russia. E3S Web Conf., 254. https://doi.org/10.1051/e3sconf/202125401024
  3. Britton, M.T., Leslie, C.A., Dandekar, A.M., McGranahan, G.H., Caboni, E. (2008). Persian walnut. In: Compendium of Transgenic Crop Plants, Blackwell Publishing Ltd. 285–300. https://doi.org/10.1002/9781405181099.k0410
  4. Carvalho, M., Ferreira, P.J., Mendes, V.S., Silva, R., Pereira, J.A., Jerónimo, C., Silva, B.M. (2010). Human cancer cell antiproliferative and antioxidant activities of Juglans regia L. Food Chem. Toxicol., 48(1), 441–447. https://doi.org/10.1016/j.fct.2009.10.043
  5. Choma, I.M., Olszowy, M., Studziński, M., Gnat, S. (2019). Determination of chlorogenic acid, polyphenols and antioxidants in green coffee by thin-layer chromatography, effect-directed analysis and dot blot – comparison to HPLC and spectrophotometry methods. J. Separat. Sci., 42(8), 1542–1549. https://doi.org/10.1002/jssc.201801174
  6. Cosmulescu, S., Trandafir, I. (2011). Seasonal variation of total phenols in leaves of walnut (Juglans regia L.). J. Med. Plants Res., 5(19), 4938–4942.
  7. Cosmulescu, S., (2013). Phenotypic diversity of walnut (Juglans regia L.) in Romania – opportunity for genetic improvement. South West. J. Hortic. Biol. Environ., 4(2), 117–126.
  8. Cosmulescu, S., Trandafir, I., Nour, V., Ionica, M., Tutulescu, F. (2014). Phenolics content, antioxidant activity and color of green walnut extracts for preparing walnut liquor. Not. Bot. Horti Agrobot. Cluj-Napoca, 42(2), 551–555. https://doi.org/10.15835/nbha4229649
  9. Dżugan, M., Miłek, M., Grabek-Lejko, D., Hęclik, J., Jacek, B., Litwińczuk, W. (2021). Antioxidant activity, polyphenolic profiles and antibacterial properties of leaf extract of various Paulownia spp. clones. Agronomy, 11(10). https://doi.org/10.3390/agronomy11102001
  10. Einali, A., Azizian-Shermeh, O., Ghasemi, A. (2018). Phytochemical screening and antimicrobial activities of Periploca aphylla Decne, Persian walnut (Juglans regia L.) and oleander (Nerium indicum Mill.) leaf extracts. J. Food Meas. Charact., 12(2), 1350–1359. https://doi.org/10.1007/s11694-018-9749-9
  11. Fernández-Agulló, A., Castro-Iglesias, A., Freire, M. S., González-Álvarez, J. (2020). Optimization of the extraction of bioactive compounds from walnut (Juglans major 209 × Juglans regia) leaves. Antioxidant capacity and phenolic profile. Antioxidants, 9(1), 4–6. https://doi.org/10.3390/antiox9010018
  12. Hamdi, A., Córdoba-Rojano, M.A., Monje-Moreno, J. M., Guillén-Izquierdo, E., Rodríguez-Arcos, R., Jiménez-Araujo, A., Muñoz-Ruiz, M.J., Guillén-Bejarano, R. (2025). Harnessing the potential of walnut leaves from Nerpio. Unveiling extraction techniques and bioactivity through Caenorhabditis elegans studies. Foods, 14(6), 10–12. https://doi.org/10.3390/foods14061048
  13. Hamidirad, M., Sarikhani, S., Nikpendar, A., Sheikhi, A., Roozban, M.R., Ghahramanzadeh, S., Vahdati, K. (2025). Unlocking the walnut genetic resources in Northeastern Iran for late-leafing and nut quality. Genet. Resour. Crop Evol., 72(5), 6129–6143. https://doi.org/10.1007/s10722-024-02324-5
  14. http://szczepionyorzech.pl. Available: https://web.archive.org/web/20161030181358/http://szczepionyorzech.pl/oferta-odmian.html [date of accedd: 30.10.2016]. [In Polish].
  15. Jakopič, J., Veberič, R., Štampar, F. (2009). Extraction of phenolic compounds from green walnut fruits in different solvents. Acta Agric. Slov., 93(1), 11–15. https://doi.org/10.2478/v10014-009-0002-4
  16. Keles, H., Akca, Y., Ercisli, S. (2014). Selection of promising walnut genotypes (Juglans regia L.) from Inner Anatolia. Acta Sci. Pol. Hortorum Cultus, 13(3), 167–175.
  17. Masek, A., Latos-Brozio, M., Chrzescijanska, E., Podsedek, A. (2019). Polyphenolic profile and antioxidant activity of Juglans regia L. leaves and husk extracts. Forests, 10(11), 1–14. https://doi.org/10.3390/f10110988
  18. Medic, A., Zamljen, T., Hudina, M., Veberic, R. (2021). Identification and quantification of naphthoquinones and other phenolic compounds in leaves, petioles, bark, roots, and buds of Juglans regia L., using HPLC-MS/MS. Horticulturae, 7(9). https://doi.org/10.3390/horticulturae7090326
  19. Miłek, M., Ciszkowicz, E., Lecka-Szlachta, K., Miłoś, A., Zaguła, G., Pasternakiewicz, A., Dżugan, M. (2022a). Mineral composition, antioxidant, anti-urease, and antibiofilm potential of juglans regia leaves and unripe fruits. Acta Univ. Cibiniensis E, Food Technol. 26(1), 69–82. https://doi.org/10.2478/aucft-2022-0006
  20. Miłek, M., Marcinčáková, D., Kolesárová, M., Legáthová, D., Dżugan, M. (2022b). The effect of adding spices to green walnut tinctures on their polyphenolic profile, antioxidant capacity and action on renal cells. Appl. Sci., 12(7), 3669. https://doi.org/10.3390/app12073669
  21. Mukarram, S.A., Wandhekar, S.S., Ahmed, A.E.M., Pandey, V.K., Oláh, C., Daróczi, L., Prokisch, J., Endre, H., Kovács, B. (2024). Exploring the ecological implications, gastronomic applications, and nutritional and therapeutic potential of Juglans regia L. (green walnut). A comprehensive review. Nutrients, 16(8), 1183. https://doi.org/10.3390/nu16081183
  22. Nour, V., Trandafir, I., Cosmulescu, S. (2013). HPLC determination of phenolic acids, flavonoids and juglone in walnut leaves. J. Chromatogr. Sci., 51(9), 883–890. https://doi.org/10.1093/chromsci/bms180
  23. Oliveira, I., Sousa, A., Ferreira, I.C.F.R., Bento, A., Estevinho, L., Pereira, J.A. (2008). Total phenols, antioxidant potential and antimicrobial activity of walnut (Juglans regia L.) green husks. Food Chem. Toxicol., 46(7), 2326–2331. https://doi.org/10.1016/j.fct.2008.03.017
  24. Pakrah, S., Rahemi, M., Nabipour, A., Zahedzadeh, F., Kakavand, F., Vahdati, K. (2021). Sensory and nutritional attributes of Persian walnut kernel influenced by maturity stage, drying method, and cultivar. J. Food Process. Preserv., 45(6), 1–12. https://doi.org/10.1111/jfpp.15513
  25. Pereira, J.A., Oliveira, I., Sousa, A., Ferreira, I.C.F.R., Bento, A., Estevinho, L. (2008). Bioactive properties and chemical composition of six walnut (Juglans regia L.) cultivars. Food Chem. Toxicol., 46(6), 2103–2111. https://doi.org/10.1016/j.fct.2008.02.002
  26. Pycia, K., Kapusta, I., Jaworska, G. (2019). Impact of the degree of maturity of walnuts (Juglans regia L.) and their variety on the antioxidant potential and the content of tocopherols and polyphenols. Molecules, 24(16), 2936. https://doi.org/10.3390/molecules24162936
  27. Rabiei, K., Ebrahimzadeh, M. A., Saeedi, M., Bahar, A., Akha, O., Kashi, Z. (2018). Effects of a hydroalcoholic extract of Juglans regia (walnut) leaves on blood glucose and major cardiovascular risk factors in type 2 diabetic patients. A double-blind, placebo-controlled clinical trial. BMC Complement. Altern. Med. 18(1), 1–7. https://doi.org/10.1186/s12906-018-2268-8
  28. Santos, A., Barros, L., Calhelha, R.C., Dueñas, M., Carvalho, A.M., Santos-Buelga, C., Ferreira, I.C.F.R. (2013). Leaves and decoction of Juglans regia L.. Different performances regarding bioactive compounds and in vitro antioxidant and antitumor effects. Ind. Crops Prod. 51, 430–436. https://doi.org/10.1016/j.indcrop.2013.10.003
  29. Sarikhani, S., Vahdati, K., Ligterink, W. (2021). Biochemical properties of superior Persian walnut genotypes originated from southwest of Iran. Int. J. Hortic. Sci. Technol., 9(2), 13–24. https://doi.org/10.22059/ijhst.2020.309363.392
  30. Savić, I.M., Savić Gajić, I.M. (2025). Extraction and characterization of antioxidants and cellulose from green walnut husks. Foods, 14(3), 409. https://doi.org/10.3390/foods14030409
  31. Schwindl, S., Kraus, B., Heilmann, J. (2017). Phytochemical study of Juglans regia L. leaves. Phytochemistry, 144, 58–70. https://doi.org/10.1016/j.phytochem.2017.08.012
  32. Sharafati-Chaleshtori, R., Sharafati-Chaleshtori, F., Rafieian, M. (2011). Biological characterization of Iranian walnut (Juglans regia) leaves. Turk. J. Biol., 35(5), 635–639. https://doi.org/10.3906/biy-1005-1
  33. Sharma, P., Ravikumar, G., Kalaiselvi, M., Gomathi, D., Uma, C. (2013). In vitro antibacterial and free radical scavenging activity of green hull of Juglans regia. J. Pharm. Anal., 3(4), 298–302. https://doi.org/10.1016/j.jpha.2013.01.006
  34. Shi, B., Zhang, W., Li, X., Pan, X. (2018). Seasonal variations of phenolic profiles and antioxidant activity of walnut (Juglans sigillata Dode) green husks. Int. J. Food Prop., 20(3), S2635–S2646. https://doi.org/10.1080/10942912.2017.1381706
  35. Srinivasulu, C., Ramgopal, M., Ramanjaneyulu, G., Anuradha, C.M., Suresh Kumar, C. (2018). Syringic acid (SA) ‒ a review of its occurrence, biosynthesis, pharmacological and industrial importance. Biomed. Pharmacother. 108, 547–557. https://doi.org/10.1016/j.biopha.2018.09.069
  36. Strugstad, M.P., Despotovski, S. (2012). A summary of extraction, synthesis, properties, and potential uses of Juglone. A literature review. J. Ecosystems Manag., 13(3), 1–16. https://doi.org/10.22230/jem.2012v13n3a119
  37. Temizyürek, F., Gündeşli, M.A., Canbay, Y., Özatar, H.O., Gölcü, E., Altun, V.C., Atagül, Ö., Güney, M., Kafkas, E., Ugur, R., Sütyemez, M. (2025). Evaluation of biochemical profiles in walnut (Juglans regia L.) genotypes and predictive modeling using advanced machine learning algorithms. Eur. Food Res. Technol., 251 3105–3122. https://doi.org/10.1007/s00217-025-04826-7
  38. Vahdati, K., Arab, M.M., Sarikhani, S., Sadat-Hosseini, M., Leslie, C.A. Brown, P.J. (2019). Advances in Persian walnut (Juglans regia L.) breeding strategies. In: Advances in plant breeding strategies. Nut and beverage crops, Al-Khayri, J., Jain, S., Johnson, D. (eds). Springer, Cham. https://doi.org/10.1007/978-3-030-23112-5_11
  39. Vieira, V., Pereira, C., Abreu, R.M.V., Calhelha, R.C., Alves, M.J., Coutinho, J.A.P., Ferreira, O., Barros, L., Ferreira, I.C.F.R. (2020). Hydroethanolic extract of Juglans regia L. green husks: A source of bioactive phytochemicals. Food Chem. Toxicol. 137, 111189. https://doi.org/10.1016/j.fct.2020.111189
  40. Wang, X., Zhao, M., Su, G., Cai, M., Sun-Waterhouse, D., Zhou, C., Lin, L. (2016). Antihyperuricemic activities of an ethanolic and aqueous extract of walnut (Juglans regia L.) shell and a new aldehyde xanthine oxidase inhibitor. Int. J. Food Sci. Technol., 51(2), 453–460. https://doi.org/10.1111/ijfs.12995
  41. Zdyb, H. (2003). Orzech włoski [Walnut]. PWRiL, Warszawa. [In Polish].

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