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Vol. 25 No. 4 (2026):

Research paper

Effects of foliar-applied fulvic acids on yield, biomass distribution, and nitrate accumulation in hydroponically grown radish (Raphanus sativus L.)

DOI: https://doi.org/10.24326/asphc.2026.5693
Submitted: 12 April 2026
Published: 28.08.2026

Abstract

Fulvic acids are humic-derived biostimulants that can modulate nutrient uptake, carbon metabolism, and biomass allocation in horticultural crops; however, their effect on assimilate partitioning and nitrate accumulation in hydroponically grown root vegetables remains poorly understood. This study evaluated the effect of weekly foliar application of a fulvic acid-based biostimulant (K-TIONIC®, 4 mL L–1) on yield, biomass distribution, nitrate content, and quality of radish (Raphanus sativus L. Crimson Giant) cultivated in an ebb-and-flow hydroponic system under greenhouse conditions in Chihuahua, Mexico. A completely randomized design with two treatments (control and fulvic acids) and 36 experimental units per treatment was used. Fulvic acid application increased equatorial diameter by 8.50%, fresh storage root weight by 28%, and total yield by 41.66%. In contrast, vegetative growth parameters and photosynthetic pigments were unaffected. Regarding quality attributes, nitrate concentration in edible tissues decreased by 4.96% and water content by 0.57%,  pH, total soluble solids, color index were not significantly affected, and visual quality index improved by 98%. These results indicate that foliar-applied fulvic acids improve radish productivity and commercial quality in soilless systems primarily by promoting biomass distribution  to the storage organ.

References

  1. Ampong, K., Thilakaranthna, M. S., Gorim, L.Y. (2022). Understanding the role of humic acids on crop performance and soil health. Front. Agron., 4, 848621. https://doi.org/10.3389/fagro.2022.848621 DOI: https://doi.org/10.3389/fagro.2022.848621
  2. Asif, A., Ali, M., Qadir, M., et al. (2023). Enhancing crop resilience by harnessing the synergistic effects of biostimulants against abiotic stress. Front. Plant Sci. https://doi.org/10.3389/fpls.2023.1276117 DOI: https://doi.org/10.3389/fpls.2023.1276117
  3. Barzegar, T., Mahmoodi, S., Nekounam, F., et al. (2022). Effects of humic acid and cytokinin on yield, biochemical attributes, and nutrient elements of radish (Raphanus sativus L.) cv. Watermelon. J. Plant Nutr., 45(10), 1582–1598. https://doi.org/10.1080/01904167.2021.2003395 DOI: https://doi.org/10.1080/01904167.2021.2003395
  4. Canellas, L.P., Olivares, F.L., Aguiar, N.O., et al. (2015). Humic and fulvic acids as biostimulants in horticulture. Sci. Hort., 196, 15–27. https://doi.org/10.1016/j.scienta.2015.09.013 DOI: https://doi.org/10.1016/j.scienta.2015.09.013
  5. Cataldo, E., Fucile, M., Mattii, G.B. (2022). Biostimulants in viticulture: A sustainable approach against biotic and abiotic stresses. Plants, 11(2), 162. https://doi.org/10.3390/plants11020162 DOI: https://doi.org/10.3390/plants11020162
  6. Colla, G., Hoagland, L., Ruzzi, M., et al. (2017). Biostimulant action of protein hydrolysates: unraveling their effects on plant physiology and microbiome. Front. Plant Sci., 9,1652. https://doi.org/10.3389/fpls.2017.02202 DOI: https://doi.org/10.3389/fpls.2017.02202
  7. Colla, G., Kim, H.J., Kyriacou, M.C., et al. (2018). Nitrate in fruits and vegetables. Sci. Hort., 237, 221–238. https://doi.org/10.1016/j.scienta.2018.04.016 DOI: https://doi.org/10.1016/j.scienta.2018.04.016
  8. CONTAM – Panel on Contaminants in the Food Chain (2008). Scientific Opinion on nitrate in vegetables. EFSA J., 6(6), 689, 1–79. https://doi.org/10.2903/j.efsa.2008.689 DOI: https://doi.org/10.2903/j.efsa.2008.689
  9. Conversa, G., Bonasia, A., Lazzizera, C., et al. (2021). Reduction of nitrate content in baby-leaf lettuce and Cichorium endivia through the soilless cultivation system, electrical conductivity and management of nutrient solutions. Front. Plant Sci., 12, 645671. https://doi.org/10.3389/fpls.2021.645671 DOI: https://doi.org/10.3389/fpls.2021.645671
  10. Cordoba-Novoa, H.A., Gómez, S.V., Ñústez, C.E. (2018). Yield and phenology evaluation of three tomato cherry genotypes (Solanum lycopersicum L.) under greenhouse conditions. Rev. Colomb. Cienc. Hortíc, 12, 113–125. https://doi.org/10.17584/rcch.2018v12i1.7348 DOI: https://doi.org/10.17584/rcch.2018v12i1.7348
  11. De Guzman, R., Coronel, J, Dumlao, M., et al. (2026). Tuber yield of radish (Raphanus sativus L.) as affected by different frequencies of hydroponic waste nutrient solution application. Intern. J. Multidisc. Appl. Bus. Educ. Res., 7(4), 1702–1707. https://doi.org/10.11594/ijmaber.07.04.18 DOI: https://doi.org/10.11594/ijmaber.07.04.18
  12. Di Gioia, F., Renna, M., Santamaria, P. (2017). Sprouts, microgreens and “baby leaf” vegetables. In: F., Yildiz, R.C., Wiley (eds.). Minimally processed refrigerated fruits and vegetables. Boston, MA: Springer US., 403–432. https://doi.org/10.1007/978-1-4939-7018-6_11 DOI: https://doi.org/10.1007/978-1-4939-7018-6_11
  13. Gamba, M., Asllanaj, E., Raguindin, P.F., et al. (2021). Nutritional and phytochemical characterization of radish (Raphanus sativus): A systematic review. Trends Food Sci. Technol., 113, 205–218. https://doi.org/10.1016/j.tifs.2021.04.045 DOI: https://doi.org/10.1016/j.tifs.2021.04.045
  14. Jamil, M., Lee, K.J., Kim, J.M., et al. (2007). Salinity reduced growth PS2 photochemistry and chlorophyll content in radish. Sci. Agric., 64, 111–118. https://doi.org/10.1590/S0103-90162007000200002 DOI: https://doi.org/10.1590/S0103-90162007000200002
  15. Kaya, G. (2025). A plant-derived biostimulant Aminolom Enzimatico® application stimulates chlorophyll content, electrolyte leakage, stomata density and root yield of radishes under salinity stress. PeerJ, 13, e18804. https://doi.org/10.7717/peerj.18804 DOI: https://doi.org/10.7717/peerj.18804
  16. Kechasov, D., Verheul, M.J., Paponov, M., et al. (2021). Organic waste-based fertilizer in hydroponics increases tomato fruit size but reduces fruit quality. Front. Plant Sci., 12, 680030. https://doi.org/10.3389/fpls.2021.680030 DOI: https://doi.org/10.3389/fpls.2021.680030
  17. Keskin, B., Akhoundnejad, Y., Dasgan, H., et al. (2025). Fulvic acid, amino acids, and vermicompost enhanced yield and improved nutrient profile of soilless iceberg lettuce. Plants, 14. https://doi.org/10.3390/plants14040609. DOI: https://doi.org/10.3390/plants14040609
  18. Lichtenthaler, H.K., Wellburn, A.R. (1983). Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem. Soc. Trans. 11(5), 591–592. https://doi.org/10.1042/bst0110591 DOI: https://doi.org/10.1042/bst0110591
  19. Liu, CJ., Lyu, C.Y., Ai, X.Z., et al. (2022). Effects of fulvic acid on photosynthetic characteristics, yield and quality of cucumber under drought stress. Ying Yong Sheng Tai Xue Bao, 33(5), 1300–1310. https://doi.org/10.13287/j.1001-9332.202205.014
  20. Lockley, R.A., Beacham, A.M., Grove, I.G., et al. (2021). Postharvest temperature and water status influence postharvest splitting susceptibility in summer radish (Raphanus sativus L.). J. Sci. Food Agric., 101(2), 536–541. https://doi.org/10.1002/jsfa.10662 DOI: https://doi.org/10.1002/jsfa.10662
  21. Lupu, M., Nedeff, V., Panainte-Lehadus, M., et al. (2024). The textural and physical characteristics of red radishes based on a puncture test. Processes, 12(2), 282. https://doi.org/10.3390/pr12020282 DOI: https://doi.org/10.3390/pr12020282
  22. Morgan, L. (2000). The pH factor in hydroponics. The Best of The Growing Edge. New Moon Publ. Inc. Corvallis, 47–51.
  23. Mosa, W., Sas-Paszt, L., Górnik, K., et al. (2021). Vegetative growth, yield, and fruit quality of guava (Psidium guajava L.) cv. Maamoura as affected by some biostimulants. BioResources. https://doi.org/10.15376/biores.16.4.7379–7399 DOI: https://doi.org/10.15376/biores.16.4.7379-7399
  24. Nardi, S., Pizzeghello, D., Schiavon, M., et al. (2016). Plant biostimulants: physiological responses induced by protein hydrolyzed-based products and humic substances in plant metabolism. Sci. Agric., 73, 18–23. https://doi.org/10.1590/0103-9016-2015-0006 DOI: https://doi.org/10.1590/0103-9016-2015-0006
  25. Saldinger, S., Rodov, V., Kenigsbuch, D., et al. (2023). Hydroponic agriculture and microbial safety of vegetables: Promises, challenges, and solutions. Horticulturae. https://doi.org/10.3390/horticulturae9010051. DOI: https://doi.org/10.3390/horticulturae9010051
  26. dos Santos, C., de Almeida, G.M., Rubio, R.S., et al. (2023). Containers and doses of controlled release fertilizer in the production of radish in a protected environment. Com. Sci., 14. https://doi.org/10.14295/CS.v14.4106 DOI: https://doi.org/10.14295/cs.v14.4106
  27. Šlosár, M., Galovičová, L., Fabianová, J., et al. (2025). Effect of humic biostimulant agriful on agronomic and nutritional parameters of radish (Raphanus sativus). Agriculture, 15(6), 595. https://doi.org/10.3390/agriculture15060595 DOI: https://doi.org/10.3390/agriculture15060595
  28. Song, H., Zhu, W., Guo, Z., et al. (2025). The impact of fulvic acid on the growth physiology, yield, and quality of tomatoes under drought conditions. Agronomy, 15(7), 1528. https://doi.org/10.3390/agronomy15071528 DOI: https://doi.org/10.3390/agronomy15071528
  29. Sun, W., Shahrajabian, M.H. (2023). The application of arbuscular mycorrhizal fungi as microbial biostimulant, sustainable approaches in modern agriculture. Plants, 12(17), 3101. https://doi.org/10.3390/plants12173101 DOI: https://doi.org/10.3390/plants12173101
  30. Tegeder, M., Masclaux‐Daubresse, C. (2018). Source and sink mechanisms of nitrogen transport and use. New Phytol., 217(1), 35–53. https://doi.org/10.1111/nph.14876 DOI: https://doi.org/10.1111/nph.14876
  31. The Jamovi Project, 2023. Jamovi (Version 2.4.8) [Computer Software].
  32. Toscano, S., Cavallaro, V., Ferrante, A., et al. (2021). Effects of different light spectra on final biomass production and nutritional quality of two microgreens. Plants, 10(8), 1584. https://doi.org/10.3390/plants10081584 DOI: https://doi.org/10.3390/plants10081584
  33. Toscano, S., Romano, D., Patanè, C. (2023). Effect of application of biostimulants on the biomass, nitrate, pigments, and antioxidants content in radish and turnip microgreens. Agronomy, 13(1), 145. https://doi.org/10.3390/agronomy13010145 DOI: https://doi.org/10.3390/agronomy13010145
  34. Uddin, R., Thakur, M., Uddin, M., et al. (2021). Study of nitrate levels in fruits and vegetables to assess the potential health risks in Bangladesh. Sci. Rep., 11(1), 4704. DOI: https://doi.org/10.1038/s41598-021-84032-z
  35. Wei, Q., Wang, J., Wang, W., et al. (2022). Identification of QTLs controlling radish root shape using multiple populations. Horticulturae, 8(10), 931. https://doi.org/10.3390/horticulturae8100931 DOI: https://doi.org/10.3390/horticulturae8100931
  36. Xu, G., Fan, X., Miller, A.J. (2012). Plant nitrogen assimilation and use efficiency. Annu. Rev. Plant Biol., 63, 153–182. https://doi.org/10.1146/annurev-arplant-042811-105532 DOI: https://doi.org/10.1146/annurev-arplant-042811-105532
  37. Yassin, A., Al-Zubaidi, N. (2025). Effect of fertilizing with agricultural sulfur and foliar feeding with humic and fulvic acids on some qualitative and quantitative characteristics in cucumber Cucumis sativus L. under protected cultivation conditions. IOP Conference Series: Earth and Environmental Science, 1487. https://doi.org/10.1088/1755-1315/1487/1/012049 DOI: https://doi.org/10.1088/1755-1315/1487/1/012049
  38. Zhang, P., Zhang, H., Wu, G., et al. (2021). Dose-dependent application of straw-derived fulvic acid on yield and quality of tomato plants grown in a greenhouse. Front. Plant Sci., 12, 736613. https://doi.org/10.3389/fpls.2021.736613 DOI: https://doi.org/10.3389/fpls.2021.736613
  39. Zhao, Z., Wang, C., Yu, X., et al. (2022). Auxin regulates source-sink carbohydrate partitioning and reproductive organ development in rice. Proc. Nat. Acad. Sci., 119(36), e2121671119. https://doi.org/10.1073/pnas.2121671119 DOI: https://doi.org/10.1073/pnas.2121671119

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