CLONAL PROPAGATION OF Gypsophila aretioides, AN IDEAL ROCK GARDEN PLANT SPECIES

Leila Samiei; Mahboubeh Davoudi Pahnekolayi; Zahra Karimian

doi:10.24326/asphc.2019.1.4

Vol. 18 No. 1 (2019)

Articles

CLONAL PROPAGATION OF Gypsophila aretioides, AN IDEAL ROCK GARDEN PLANT SPECIES

Leila Samiei⁺⁻
Mahboubeh Davoudi Pahnekolayi⁺⁻
Zahra Karimian⁺⁻

pdf

DOI: https://doi.org/10.24326/asphc.2019.1.4
Submitted: February 19, 2019
Published: 2019-02-19

Abstract

Gypsophila aretioides, a cushion form evergreen plant, is a high potential wild species ideal for the use in rock garden, or as a ground cover in sunny dry areas. This plant has the competence to be developed as a new ornamental species. The purpose of this experiment was to provide an efficient micropropagation protocol for G. aretioides in order to facilitate the availability of this species for further studies of domestication. The influence of various concentrations of 6-benzylaminopurine (BAP) and thidiazuron (TDZ) was investigated for multiplication stage. TDZ at low concentration of 0.05 mg dm⁻³ resulted in the maximum shoot (9.7) and leaf (42.3) number. The shoots were best rooted on MS medium containing 0.6 mg dm^–3 indolebutyric acid (IBA) with 7.8 roots per shoot. Despite achievement of a successful protocol for in vitro multiplication and root induction of Gypsophila, low survival rate was obtained when rooted explants were exposed to ex vitro conditions. This is an important issue, which requires particular consideration and further studies. The possible reasons contributing to the low acclimatization rate of this species are being discussed.

References

Ahmad, N., Anis, M. (2007). Rapid clonal multiplication of a woody tree, Vitex negundo L. through axillary shoots proliferation. Agrofor. Syst., 71,195–200.
Ahmad, N., Faisal, M., Anis, M. (2013). Role of PGR on in vitro shoot propagation in Cyamopsis tetragonoloba L. (Taub.): a drought tolerant grain legume. Rend. Lincei, 24, 7–12.
Ahroni, A., Zuker, A., Rozen, Y., Shejtman, H., Vainstein, A. (1997). An efficient method for adventitious shoot regeneration from stem-segment explants of Gypsophila. Plant Cell Tiss. Org., 49, 101–106.
Chandra, S., Bandopadhyay, R., Kumar, V., Chandra, R. (2010). Acclimatization of tissue cultureed plantlets: from laboratory to land. Biotechnol. Lett., 32, 1199–1205.
Daniels, S.J. (1994). The physiology of Dionysa aretiodes during micropropagation. Durham University.
Davoudi Pahnekolay, M., Samiei, L., Tehranifar, A., Shoor, M. (2015). The effect of medium and plant growth regulators on micropropagation of Dog rose (Rosa canina L.). J. Plant Mol. Breed., 3, 61–71.
Desjardins, Y., Dubuc J.F., Badr, A. (2009). In vitro culture of plants: a stressful activity! Acta Hortic., 812, 29–50.
Dewir, Y.H., Indoliya, Y., Chakrabarty, D., Paek, K.Y. (2014). Biochemical and physiological aspects of hyperhydricity in liquid culture system. In: Production of biomass and bioactive compounds using bioreactor technology, Paek, K.Y., Murthy, H.N., Zhong, J.J. (eds.). Springer, Berlin, 693–703.
Faisal, M., Alatar, A., Ahmad, N., Anis, M., Hegazy, A.K. (2012). An efficient and reproducible method for in vitro clonal multiplication of Rauvolfia tetraphylla L. and evaluation of genetic stability using DNA-based markers. Appl. Biochem. Biotechnol., 168, 1793–1752.
Fraga, M., Alonso, M., Ellul, P., Borja, M. (2004). Micropropagation of Dianthus gratianopolitanus. Hortscience, 39, 1083–1087.
Gantait, S., Mandal, N., Bhattacharyya, S., Das, P.K. (2010). An elite protocol for accelerated quality-cloning in Gerbera jamesonii Bolus cv. Sciella. In Vitro Cell Dev. Biol. Plant, 46, 537–548.
Goncalves, S., Martins, N., Romano, A. (2017) Physiological traits and oxidative stress markers during acclimatization of micropropagated plants from two endangered Plantago species: P. algarbiensis Samp. and P. almogravensis Franco. In Vitro Cell. Dev. Biol. Plant, 53, 249–255.
Han, B.H., Yu, H.J., Yae, B.W., Peak, K.Y. (2004). In vitro micropropagation of Lilium longiflorum ‘Georgia’ by shoot formation as influenced by addition of liquid medium. Sci. Hortic., 103, 39–49.
Hare, P.D., Van Staden, J. (1994). Inhibitory effect of thidiazuron on the activity of cytokinin oxidase isolated from soybean callus. Plant Cell Physiol., 35,1121–1125.
Hazarika, B.N. (2003). Acclimatization of tissue-cultured plants. Curr. Sci., 85, 1704–1712.
Huetteman, C.A., Preece, J.E. (1993). Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss. Org., 33, 105–119.
Kiani, M., Zarghami, H., Memariani, F., Tehranifar, A. (2013). In vitro propagation and conservation of Diaphanoptera khorasanica (Caryophyllaceae), a threatened endemic and potential ornamental species in northeast of Iran. J. Cell Mol. Res., 4, 89–96.
Kritskaya, T., Kashin, A., Spivak, V., Firstov, V. (2016). Features of clonal micropropagation of Silene cretacea (Caryophyllaceae) in in vitro culture. Russ. J. Dev. Biol., 47, 359–366.
Meiners, J., Schwab, M., Szankowski, I. (2007) Efficient in vitro regeneration systems for Vaccinium species. Plant Cell Tiss. Org., 89, 169–176.
Melzheimer, V. (1980). Caryophyllaceae. In: Flora Iranica, No.163, Rechinger, K.H. (ed.). Akademische Druck-U. Verlagsanstalt, Austria, 353–508.
Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15, 473–497.
Nhut, D.T., Hai, N.T., Phan, M.X. (2010). A highly efficient protocol for micropropagation of Begonia tuberous. In: Protocols for in vitro propagation of ornamental plants, Jain, S.M., Ochatt, S.J. (eds.). Springer, New York−Dordrecht−Heidelberg−London, 15–20.
Padmanabhan, P., Murch, S.J., Sullivan, J.A., Saxena, P.K. (2015). Micropropagation of Primulina dryas (Dunn) Mich. Möller & A. Webber: High frequency regeneration from leaf explants. Sci. Hortic., 192, 250–255.
Parveen, S., Shahzad, A. (2010). TDZ-induced high frequency shoot regeneration in Cassia sophera Linn. via cotyledonary node explants. Physiol. Mol. Biol. Plants, 16, 201–206.
Posposilova, J., Ticha, I., Kadlecek, P., Haisel, D., Plzakova, S. (1999). Acclimatization of micropropagated plants to ex vitro conditions. Biol. Plant., 42, 481–497.
Prakash, J. (2009). Micropropagation of ornamental perennials: progress and problems. Acta Hortic., 812, 289–294.
Rady, M. (2006). In vitro culture of Gypsophila paniculata L. and random amplified polymorphic DNA analysis of the propagated plants. Biol. Plant., 50, 507–513.
Rai, M.K., Shekhawat, N.S., Gupta, A.K., Phulwaria, M., Ram, K., Jaiswal, U. (2011). The role of abscisic acid in plant tissue culture: a review of recent progress. Plant Cell Tiss. Org., 106, 179–190.
Samiei, L., Tehranifar, A., Aghdaei, M., Vessal, S. (2017). Control of in vitro contaminations in Dionysia tapetodes for effective micropropagation. J. Ornam. Plants., 7(4), 223–228.
Shaik, N.M., Arha, M., Nookaraju, A., Gupta, S.K., Srivastava, S., Yadav, A.K., Kulkarni, P.S. Abhilash, O., Vishwakarma, R.K., Singh, S. (2009). Improved method of in vitro regeneration in Leucaena leucocephala – a leguminous pulpwood tree species. Physiol. Mol. Biol. Plants, 15, 311–318.
Singh, C.K., Raj, S.R., Jaiswal, P.S., Patil, V.R., Punwar, B.S., Chavda, J.C., Subhash, N. (2016). Effect of plant growth regulators on in vitro plant regeneration of sandalwood (Santalum album L.) via organogenesis. Agrofor. Syst., 90, 281–288.
Smith, M., Mcclelland, M., Timmermann, R. (1991). Anomalous root structure on woody plants in vitro. J. Environ. Hortic. (USA), 9, 61–64.
Wochok, Z.S. (1981). The role of tissue culture in preserving threatened and endangered plant species. Biol. Conserv., 20, 83–89.
Zuker, A., Ahroni, A., Shejtman, H., Vainstein, A. (1997). Adventitious shoot regeneration from leaf explants of Gypsophila paniculata L. Plant Cell Rep., 16, 775–778.