Abstrakt
In the preceding research, stevia has been typically cloned in vitro using two media, on which the shoots were formed (3–6 weeks), and on the other they were rooted (3–5 weeks). This study aimed at finding the possibility for rapid stevia propagation from large nodal explants using the MS basal medium [Murashige and Skoog 1962], with low auxin concentrations (0.5, 1 and 2 mg‧dm–3). The plants were obtained as soon as after three weeks. The best results were obtained from media with various concentrations of the indole-3-acetic acid (IAA) and the highest concentration of phenylacetic acid (PAA). Plants were formed by 83.9-86.0% of explants, they had high weight (234−253 mg), two shoots measuring 2.07−2.37 cm and 5.8−8.3 roots measuring 1.00–1.24 cm. Mean plant weight was lowest on the media with indole-3-butyric acid (IBA) (185–192 mg). Both explant buds formed single shoots, but their development was typically uneven. The differences in the length and weight of shoots were lowest on the media with IAA and at lower PAA concentrations. Plants from the media with IAA and the control medium were distinguished by a higher number of nodes. The percentage share of shoots in the total plant weight was highest on the media with PAA (62.1–62.7%), and lowest at higher concentrations of α-naphthaleneacetic acid (NAA) (47.9 and 48.9%). Parts of explants immersed in media developed callus, and the highest amounts of this tissue were found in the media with NAA. 92.3% of plants survived the acclimatization. The applied procedure may be used for rapid in vitro cloning of selected stevia genotypes. The use of one medium enables reduction of seedling production costs. Moreover, cyclical cloning and extending the production scale is possible.
Bibliografia
- Ahmed, M.B., Salahin, M., Karim, R., Razvy, M. A., Hannan, M.M., Sultana, R., Hossain, M., Islam, R. (2007). An efficient method for in vitro clonal propagation of a newly introduced sweetener plant (Stevia rebaudiana Bertoni) in Bangladesh. Am.-Euras. J. Sci. Res., 2(2), 121–125.
- Anbazhagan, M., Kalpana, M., Rajendran, R., Natarajan, V., Dhanavel, D. (2010). In vitro production of Stevia rebaudiana Bertoni. Emir. J. Food Agric., 22(3), 216–222.
- Brandle, J.E., Starratt, A.N., Gijzen, M. (1998). Stevia rebaudiana: its agricultural, biological and chemical properties. Can. J. Plant Sci., 78, 527–536.
- Carneiro, J.W.P., Muniz, A.S., Guedes, T.A. (1997). Greenhouse bedding plant production of Stevia rebaudiana (Bert) bertoni. Can. J. Plant Sci., 77, 473–474.
- Das, A., Gantait, S., Mandal, N. (2011). Micropropagation of an elite medicinal plant: Stevia rebaudiana Bert. Int. J. Agric. Res., 6(1), 40–48.
- Driver, J.A., Kuniyuki, A.H. (1984). In vitro propagation of Paradox walnut rootstock. Hort. Sci., 19, 507–509.
- Genus, J.M., Buyse, J., Vankeirsbilck, A., Temme, E.H. (2007). Metabolism of stevioside by healthy subjects. Exp. Biol. Med., 232, 163–173.
- Grenby, T.H. (1991). Update on low-calorie sweeteners to benefit dental health. Int. J. Dent., 41, 217–224.
- Huxtable, R.J. (2002). Pharmacology and toxicology of stevioside, rebaudioside A, and steviol. In: Stevia, the genus Stevia. Medicinal and aromatic plants − industrial profiles, Kinghorn A. D. (Ed.). Vol. 19. Tylor Francis Group, London−New York, 160–178.
- Khalil, S.A., Zamir, R., Ahmed, N. (2014). Selection of suitable propagation method for consistent plantlets production in Stevia rebaudiana (Bertoni). Saudi J. Biol. Sci., 21, 566–573.
- Lata, H., Chandra, S., Wang, Y.H., Raman, V., Khan, I.A. (2013). TDZ-induced high frequency plant regeneration through direct shoot organogenesis in Stevia rebaudiana Bertoni: an important medicinal plant and a natural sweetener. Am. J. Plant Sci., 4, 117–128.
- Lemus-Mondaca, R., Vega-Galez, A., Zura-Bravo, L., Ah-Hen, K. (2012). Stevia rebaudiana Bertoni, source of a high-potency natural sweetener: A comprehensive review on the biochemical, nutritional and functional aspects. Food Chem., 132, 1121–1132.
- Lloyd, G., McCown, B. (1980). Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shot-tip culture. Inter Plant Prop. Soc. Proc., 30, 421–445.
- Mathur, M., Begum, T. (2015). Shootles regeneration and tissue culture studies on Stevia rebaudiana Bertoni and Terminalia bellerica Roxb. Int. J. Rec. Biotech., 3(1), 25–35.
- Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15, 473–497.
- Peixe, A., Calado, J., Carlos, R. (2015). A one-step procedure for in vitro micropropagation of stevia (Stevia rebaudiana Bertoni). Acta Hortic., 1083, 295–301.
- Rafiq, M., Dahot, M.U., Mangrio, S.M., Naqvi, H.A., Qarshi, I.A. (2007). In vitro clonal propagation and biochemical analysis of field established Stevia rebaudiana Bertoni. Pak. J. Bot., 39(7), 2467–2474.
- Ramesh, K., Singh, V., Megeji, N.W. (2006). Cultivation of Stevia [Stevia rebaudiana (Bert.) Bertoni]: a comprehensive review. Adv. Agron., 89, 137–177.
- Razak, U.N.A.A., Ong, C.B., Yu, T.S., Lau, L.K. (2014). In vitro micropropagation of Stevia rebaudiana Bertoni in Malaysia. Braz. Arch. Biol. Technol., 57(1), 23–28.
- Rosales, C., Brenes, J., Salas, K., Arce-Solano, S., Abdelnour-Esquivel, A. (2018). Micropropagation of Stevia rebaudiana in the temporary immersion systems as an alternative horticultural production method. Rev. Chapingo Ser. Horticultura 24(1), 69–84.
- Savita, S.M., Shella, K., Sunanda, S., Shankar, A.G., Ramakrishna, P. (2004). Stevia rebaudiana – a functional component for food industry. J. Human Ecol. 15, 261–264.
- Soejarto, D.D. (2002). Botany of Stevia rebaudiana. In: Stevia, the genus Stevia. Medicinal and aromatic plants − industrial profiles, Kinghorn A. D. (Ed.). Vol. 19. Tylor Francis Group, London−New York, 18−39.
- Taleje, N., Hamidoghli, S., Hamidoghli, Y. (2012). In vitro plantlet propagation of Stevia rebaudiana Bertoni. South West. J. Hortic. Biol. Environ., 3(1), 99–108.
- Tateo, F., Mariotti, M., Bononi, M., Lubian, E., Martello, S., Cornara, L. (1998). Stevioside content and morphological variability in a population of Stevia rebaudiana (Bertoni) from Paraguay. Ital. J. Food. Sci. 10(3), 261–267.
- Verma, S., Yadav, K., Singh, N. (2011). Optimization of the protocols for surface sterilization, regeneration and acclimatization of Stevia rebaudiana Bertoni. Am.-Eur. J. Agric. Environ. Sci., 11(2), 221–227.
- Yücesan, B., Büyükgöçmen, R., Mohammed, A., Sameeullah, M., Altuğ, C., Gürel, S., Gürel, E. (2016). An efficient regeneration system and steviol glycoside analysis of Stevia rebaudiana Bertoni, a source of natural high-intensity sweetener. In Vitro Cell. Dev. Biol.-Plant, 52, 330–337.
Downloads
Download data is not yet available.
-
Natalia Dimitrova,
Lilyana Nacheva,
Diyana Aleksandrova,
Marieta Nesheva,
Małgorzata Berova,
Effects of meta–topolin riboside and meta–methoxy topolin riboside on the in vitro micropropagation of Pyrus communis L.
,
Acta Scientiarum Polonorum Hortorum Cultus: ONLINE FIRST
-
Natalia Miler,
Iwona Jędrzejczyk,
Paweł Trafara,
Janusz Winiecki,
Effect of high-energy ionizing radiation on the DNA content and genetic variation in chrysanthemum plants regenerated from irradiated ovaries
,
Acta Scientiarum Polonorum Hortorum Cultus: Tom 22 Nr 4 (2023)
-
Aboubaker H. Brayek,
Ranko R. Čabilovski,
Klara M. Petković,
Nenad P. Magazin,
Dragan B. Čakmak,
Maja S. Manojlovic,
EFFICIENCY OF DIFFERENT METHODS AND FORMS OF MICROELEMENTS APPLICATION IN FUNCTION OF N FERTILIZER IN APPLE TREES
,
Acta Scientiarum Polonorum Hortorum Cultus: Tom 18 Nr 5 (2019)
-
Joanna Gałązka,
Renata Słomnicka,
Katarzyna Góral-Radziszewska,
Katarzyna Niemirowicz-Szczytt,
FROM POLLINATION TO DH LINES – VERIFICATION AND OPTIMIZATION OF PROTOCOL FOR PRODUCTION OF DOUBLED HAPLOIDS IN CUCUMBER
,
Acta Scientiarum Polonorum Hortorum Cultus: Tom 14 Nr 3 (2015)
-
Fusun Yurekli,
Oguz A. Kirecci,
Ilknur Celik,
THE EFFECTS OF NITRIC OXIDE ON SOME ANTIOXIDANT ENZYME ACTIVITIES UNDER SALT STRESS IN SUNFLOWER PLANTS
,
Acta Scientiarum Polonorum Hortorum Cultus: Tom 18 Nr 5 (2019)
-
Sevinç Kiran,
ALLEVIATION OF ADVERSE EFFECTS OF SALT STRESS ON LETTUCE (Lactuca sativa var. crispa) BY APPLICATION OF VERMICOMPOST
,
Acta Scientiarum Polonorum Hortorum Cultus: Tom 18 Nr 5 (2019)
-
Barbara Marcinek,
Marzena Parzymies,
Monika Poniewozik,
Danuta Kozak,
Wojciech Durlak,
THE INFLUENCE OF GROWTH REGULATORS ON DAHLIA PROPAGATION IN TISSUE CULTURE AND ACCLIMATIZATION OF PLANTS IN ex vitro CONDITIONS
,
Acta Scientiarum Polonorum Hortorum Cultus: Tom 18 Nr 5 (2019)
-
Andrzej Kalisz,
Agnieszka Sękara,
Aneta Grabowska,
Konrad Sulak,
Ewa Capecka,
Andrzej Libik,
Rita Jurkow,
Bogdan Kulig,
PERFORMANCE OF BIODEGRADABLE FLOATING DIRECT COVERS IN THE FIELD PRODUCTION OF BUTTERHEAD LETTUCE DURING SPRING AND AUTUMN TRIALS
,
Acta Scientiarum Polonorum Hortorum Cultus: Tom 20 Nr 2 (2021)
-
Anastasia A. Papadaki,
Kalliopi Ladomenou,
Heavy metal accumulation in various tissues of radish (Raphanus sativus) grown under different ratios of organic amendments
,
Acta Scientiarum Polonorum Hortorum Cultus: Tom 18 Nr 4 (2019)
-
Tugce OZSAN,
Elif Gulsun VURAL,
Ahmet Naci ONUS,
DO JASMONIC ACID AND ACTIVATED CHARCOAL INCREASE THE in vitro DEVELOPMENT OF ORANGE CARROT (Daucus carota L. subsp. sativus Hoffm.) AND PURPLE CARROT (Daucus carota L. ssp. sativus var. atrorubens Alef.)?
,
Acta Scientiarum Polonorum Hortorum Cultus: Tom 19 Nr 5 (2020)
<< < 14 15 16 17 18 19 20 21 22 23 > >>
Możesz również Rozpocznij zaawansowane wyszukiwanie podobieństw dla tego artykułu.