Agronomy Science, przyrodniczy lublin, czasopisma up, czasopisma uniwersytet przyrodniczy lublin

Effect of increasing doses of zinc in combination with organic materials on the occurrence of entomopathogenic fungi in the soil

Anna Barbara Majchrowska-Safaryan

Institute of Agriculture and Horticulture, Faculty of Agricultural Sciences, University of Siedlce, Prusa Street 14, 08-110 Siedlce, Poland

Cezary Tkaczuk

Instytut Rolnictwa i Ogrodnictwa, Uniwersytet Przyrodniczo-Huanistyczny w Siedlcach

Beata Kuziemska

Instytut Rolnictwa i Ogrodnictwa, Uniwersytet Przyrodniczo-Huanistyczny w Siedlcach

Eustachio Tarasco

Department of Soil, Plant and Food Sciences, Section of Entomology and Zoology, University of Bari


The aim of the study was to determine the effect of zinc application in different doses with organic fertilization on the genera composition and the number of CFU (colony-forming units) of entomopathogenic fungi (EPF) in the soil. The experiment was carried out in greenhouse condi-tions. Soil samples for testing were collected in the third year of the experiment (spring, autumn), where the research objects were I – dose of zinc: control – lack (0) and 200, 400 and 600 mg Zn kg–1 of soil; II – no fertilization – 0 (CO), spent mushroom substrate (SMS), chicken (ChM) and cattle manure (CM). Entomopathogenic fungi were isolated using the method of isolation on a selective medium. In the course of the research, EPF of the genus: Beauveria, Metarhizium, Cordyceps and Lecanicillium were determined. The conducted research showed that entomopatho-genic fungi of the Metarhizium genera formed the most CFU in spring. In the autumn there were three times less of them. Statistical analysis showed that the number of CFUs of the identified genus of fungi (on average) in soil samples significantly depended on the dose of zinc applied, organic fertilization and the genus of fungus, but only for Metarhizium spp.

Słowa kluczowe:

zinc, organic materials, entomopathogenic fungi, CFU

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Anna Barbara Majchrowska-Safaryan 
Institute of Agriculture and Horticulture, Faculty of Agricultural Sciences, University of Siedlce, Prusa Street 14, 08-110 Siedlce, Poland
Cezary Tkaczuk 
Instytut Rolnictwa i Ogrodnictwa, Uniwersytet Przyrodniczo-Huanistyczny w Siedlcach
Beata Kuziemska 
Instytut Rolnictwa i Ogrodnictwa, Uniwersytet Przyrodniczo-Huanistyczny w Siedlcach
Eustachio Tarasco 
Department of Soil, Plant and Food Sciences, Section of Entomology and Zoology, University of Bari


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