The influence of toxic concentration of Cu2+ ions on the MnSOD gene expression in common wheat, triticale and rye plants
MICHAŁ NOWAK
Instytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie ul. Akademicka 15, 20-950 LublinAbstract
The basic mechanism of the toxic effect of copper ions on plant cells is reactive oxygen species (ROS) generation and oxidative stress. The first line of live cells defense against ROS is superoxide dismutase (SOD) enzymes class. Enhancement of the expression of superoxide dismutases encoding genes is one of the main mechanisms of live cell defense against oxidative stress. The aim of the paper was determination of the changes in mitochondrial manganese superoxide dismutase gene transcription level during abiotic stress for three crops: common wheat, triticale and rye. The studies showed that modification of the MnSOD gene expression is a part of response to abiotic stress caused by toxic copper ions activity in cereals. However, significant differences between analyzed species were noticed.
Keywords:
mitochondrial manganese superoxide dismutase (MnSOD), abiotic stress, common wheat, triticale, ryeReferences
Baek K.H., Skinner D.Z., 2004. Quantitative real-time PCR method to detect changes in specific transcript and total RNA amounts. Electron. J. Biotechnol. 7(1), 55–60.
Baek K.H., Skinner D.Z., Ling P., Chen X., 2006. Molecular structure and organization of the wheat genomic manganese superoxide dismutase gene. Genome 49, 209–218.
Bowler C., Alliotte T., Van Den Bulcke M., Bauw G., Vandekerckhove J., Van Montagu M., Inze D., 1989. A plant manganese superoxide dismutase is efficiently imported and correctly processed by yeast mitochondria. Proc. Natl. Acad. Sci. USA 86, 3237–3241.
Cheng H.Y., Song S.Q., 2006. Species and organ diversity in the effects of hydrogen peroxide on superoxide dismutase activity in vitro. J. Integr. Plant Biol. 48(6), 672–678.
Chomczyński P., Sacchi N., 1987. Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156–159.
Fernandes J.C., Henriques F.S., 1991. Biochemical, physiological and structural effects of excess copper in plants. Bot. Rev. 57(3), 246–273.
Fink R.C., Scandalios J.G., 2002. Molecular evolution and structure-function relationships of the superoxide dismutase gene families in angiosperms and their relationship to other eukaryotic and prokaryotic superoxide dismutases. Arch. Biochem. Biophys. 399(1), 19–36.
Lai L.S., Chang P.C., Chang C.T., 2008. Isolation and characterization of superoxide dismutase from wheat seedlings. J. Agric. Food Chem. 56, 8121–8129.
Luo H., Li H., Zhang X., Fu J., 2011. Antioxidant responses and gene expression in perennial ryegrass (Lollium perenne L.) under cadmium stress. Ecotoxicology 20, 770–778.
Mittler R., 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci. 7, 405–410.
Møller M., 2001. Plant mitochondria and oxidative stress: Electron transport, NADPH turnover, and metabolism of reactive oxygen species. Annu. Rev. Plant Physiol. Plant Mol. Biol. 52, 561–591.
Murzaeva S.V., 2004. Effect of heavy metals on wheat seedlings: activation of antioxidant enzymes. Appl. Biochem. Microbiol. 40(1), 98–103.
Naydenov N.G., Khanam S., Siniauskaya M., Nakamura C., 2010. Profiling of mitochondrial transcriptome in germinating wheat embryos and seedlings subjected to cold, salnity and osmotic stresses. Genes Genet. Syst. 85, 31–42.
Neuman P.R., Hart G.E., 1986. Genetic control of the mitochondrial form of superoxide dismutase in hexaploid wheat. Biochem. Genet. 24(5–6), 435–446.
Panda S.K., Matsumoto H., 2010. Changes in antioxidant gene expression and induction of oxidative stress in pea (Pisum sativum L.) under Al stress. Biometals 23, 753–762.
Schweizer P., 2008. Tissue-specific expression of a defence-related peroxidase in transgenic wheat potentiates cell death in pathogen-attacked leaf epidermis. Mol. Plant Pathol. 9(1), 45–57.
Untergasser A., Nijveen H., Rao X., Bisseling T., Geurts R., Leunissen J.A.M., 2007. Primer3Plus, an enhanced web interface to Primer3. Nucleic Acids Res. 35, W71–W74.
Wu G., Wilen R.W., Robertson A.J., Gusta L.V., 1999. Isolation, chromosomal localization, and differential expression of mitochondrial manganese superoxide dismutase and chloroplastic copper/zinc superoxide dismutase genes in wheat. Plant Physiol. 120, 513–520.
Yuan J.S., Reed A., Chen F., Neal Stewart Jr C., 2006. Statistical analysis of real-time PCR data. BMC Bioinformatics 7, 85.
Instytut Genetyki, Hodowli i Biotechnologii Roślin, Uniwersytet Przyrodniczy w Lublinie ul. Akademicka 15, 20-950 Lublin
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