Regulation of mitochondrial manganese superoxide dismutase (MnSOD) gene expression in cereals by copper and manganese excess

MICHAŁ NOWAK; JUSTYNA LEŚNIOWSKA-NOWAK; MAGDALENA SOZONIUK

doi:10.24326/as.2020.2.5

Tom 75 Nr 2 (2020)

Artykuły

Zmiana ekspresji genu mitochondrialnej manganowej dysmutazy ponadtlenkowej (MnSOD) w zbożach pod wpływem miedzi i manganu

MICHAŁ NOWAK⁺⁻
JUSTYNA LEŚNIOWSKA-NOWAK⁺⁻
MAGDALENA SOZONIUK⁺⁻

PDF (English)

DOI: https://doi.org/10.24326/as.2020.2.5
Przesłane: 25 listopada 2019
Opublikowane: 17-07-2020

Abstrakt

Spośród wielu różnych działań cytotoksycznych metali ciężkich w komórkach roślinnych jedno z najważniejszych wiąże się z wytwarzaniem reaktywnych form tlenu (ROS). Mechanizm obrony komórek roślinnych przed ROS i wolnymi rodnikami ma kompleksowy charakter. Celem prezentowanej pracy jest charakterystyka zmian w poziomie transkryptów genu mitochondrialnej manganowej dysmutazy ponadtlenkowej (MnSOD), które wystąpiły w siewkach pszenicy zwyczajnej (Triticum aestivum L.) i jęczmienia zwyczajnego (Hordeum vulgare L.) podczas traktowania ich miedzią i manganem. W badanym materiale w większości przypadków obserwowano obniżenie ekspresji genu MnSOD w wyniku stresu oksydacyjnego wywołanego nadmiarem miedzi. Z kolei traktowanie siewek nadmiarem manganu powodowało zróżnicowaną reakcję, co wskazuje na dużą rolę tła genetycznego badanych odmian w odpowiedzi na wywołane warunki stresowe.

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