GENETIC DIVERSITY OF Brassica rapa GERMPLASM OF KHYBER PAKHTUNKHWA PAKISTAN REVEALED BY MOLECULAR MARKERS


Abstract

A total of 96 indigenous Brassica rapa accessions were collected from different locations of Khyber Pakhtunkhwa, Pakistan. Simple Sequence Repeats (SSR) markers were used to identify the most diverse genotypes among the collected lots. Twenty six (26) different SSR primers were used for (genetic) variability among collected genotypes. These primers were selected from literature based on their previous results. These primers produced 135 scorable bands of which 75 were polymorphic, with an average of 55.5% polymorphic loci, and reflected the broader genetic background of the collected genotypes. An average 2.88 polymorphic bands with an average PIC value of 0.49 was recorded. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) divided all genotypes into three main groups. Group one contained three clusters, while group two and three had four and two clusters each. Based on the UPGMA dendrogram, genotypes collected from Kohat, Bannu, Swat and Haripur showed considerable amount of variation. From the present study, it is concluded that SSR markers can be proved as the best tool for the genetic variability of other local and exotic B. rapa genotypes.


Keywords

Brassica rapa; genetic variability; Khyber Pakhtunkhwa; polymorphic bands; simple sequence repeats; Pakistan

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Published : 2019-12-17


Ali, N., Ali, S., Khan, N., Jan, S., Rabbani, M., & Hussain, I. (2019). GENETIC DIVERSITY OF Brassica rapa GERMPLASM OF KHYBER PAKHTUNKHWA PAKISTAN REVEALED BY MOLECULAR MARKERS. Acta Scientiarum Polonorum Hortorum Cultus, 18(6), 57-65. https://doi.org/10.24326/asphc.2019.6.6

Naushad Ali 
Department of Plant Breeding & Genetics, University of Haripur, Khyber Pakhtunkhwa, Pakistan  Pakistan
Sardar Ali 
Department of Plant Breeding & Genetics, University of Haripur, Khyber Pakhtunkhwa, Pakistan  Pakistan
Naqib Ullah Khan 
Department of Plant Breeding and Genetics, The University of Agriculture Peshawar, Pakistan  Pakistan
Sohail Ahmad Jan  sjan.parc@gmail.com
Department of Biotechnology, Hazara University, Mansehra, Khyber Pakhtunkhwa, Pakistan  Pakistan
Malik Ashiq Rabbani 
Bio-Resources Conservation Institute, NARC, Islamabad, Pakistan  Pakistan
Ijaz Hussain 
Department of Horticulture, University of Haripur, Khyber Pakhtunkhwa, Pakistan  Pakistan


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