Skip to main navigation menu Skip to main content Skip to site footer
ASPHC Baner

Vol. 18 No. 6 (2019)

Articles

GENETIC DIVERSITY OF Brassica rapa GERMPLASM OF KHYBER PAKHTUNKHWA PAKISTAN REVEALED BY MOLECULAR MARKERS

DOI: https://doi.org/10.24326/asphc.2019.6.6
Submitted: December 17, 2019
Published: 2019-12-17

Abstract

A total of 96 indigenous Brassica rapa accessions were collected from different locations of Khyber Pakhtunkhwa, Pakistan. Simple Sequence Repeats (SSR) markers were used to identify the most diverse genotypes among the collected lots. Twenty six (26) different SSR primers were used for (genetic) variability among collected genotypes. These primers were selected from literature based on their previous results. These primers produced 135 scorable bands of which 75 were polymorphic, with an average of 55.5% polymorphic loci, and reflected the broader genetic background of the collected genotypes. An average 2.88 polymorphic bands with an average PIC value of 0.49 was recorded. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) divided all genotypes into three main groups. Group one contained three clusters, while group two and three had four and two clusters each. Based on the UPGMA dendrogram, genotypes collected from Kohat, Bannu, Swat and Haripur showed considerable amount of variation. From the present study, it is concluded that SSR markers can be proved as the best tool for the genetic variability of other local and exotic B. rapa genotypes.

References

  1. Anderson, J.A., Churchil, G.A., Autrique, J.E., Tanksley, S.O., Sorrels, M.E. (1993). Optimizing parent selection for genetic linkage maps. Genome, 36, 181–186.
  2. Anjum, M.A., Rauf, A., Bashir, M.A., Ahmad, R. (2018). The evaluation of biodiversity in some indigenous Indian jujube (Zizyphus mauritiana) germplasm through physico-chemical analysis. Acta Sci., Pol. Hortorum Cultus, 17(4), 39–52. DOI: 10.24326/asphc.2018.4.4
  3. Axelsson, T., Bowman, C.M., Sharpe, A.G., Lyndiate, D.J., Lagercrantz, G. (2000). Amphidiploid Brassica juncea contains conserved progenitor genomes. Genome, 43, 679–788.
  4. Cansian, R.L. Echeverrigaray, S. (2000). Discrimination among cultivars of cabbage using randomly amplified polymorphic DNA markers. Hortic. Sci, 35, 1155–1158.
  5. Das, R.S.J., Bhatia, S., Srivastava, P.S., Lakshmikumaran, M. (1999). Assessment of genetic variation within Brassica campestris cultivars using AFLP and RAPD markers. J. Biol. Sci., 24, 433–440.
  6. Doyle, J.J. Doyle, J.L. (1990). Isolation of plant DNA from fresh tissue. Focus, 12, 13–15.
  7. Gawroński, J., Kaczmarska, E., Dyduch-Siemińska, M. (2017). Assessment of genetic diversity between Vaccinium corymbosum L. cultivars using RAPD and ISSR markers. Acta Sci. Pol. Hortorum Cultus, 16(3), 129–140. DOI: 10.24326/asphc.2017.3.13
  8. Gupta, Z., Sajad, M., Moni, G., Gupta, S.K. (2014). Assessment of genetic variation in Indian mustard (Brassica juncea L.) using PCR based markers. Mol. Plant Breed., 5, 10–17.
  9. Hammer, M.F., Karafet, M.T., Redd, A.J., Jarjanazi, H., Zegura, S.L. (2001). Hirarchical patterns of global human Y-chromosome diversity. Mol. Biol. Evol., 18, 1189–1203.
  10. Hasan, M., Seyis, F., Badani, A.G., Snowdon, R.J. (2006). Analysis of genetic diversity in the Brassica napus L. gene pool using SSR markers. Genet. Resour. Crop Evol., 53, 793–802.
  11. Jan, S.A., Shinwari, Z.K., Ali, N., Rabbani, M.A. (2018). Morphometric analysis of Brassica carinata elite lines reveals variation for yield related traits. Pak. J. Bot., 50(4), 1521–1524.
  12. Khan, Q., Khan, A.S., Khurshid, H., Arif, M. (2016). Exploring durable genetic resistance against leaf rust through phenotypic characterization and LR34 linked STS marker in wheat germplasm. Biosci. J., 32(4), 986–998.
  13. Lanner, H.C., Gustafsson, M., Falt, A.S., Bryngelsson, T. (1996). Diversity in natural populations of wild Brassica oleracea as estimated by isozyme and RAPD analysis. Genet. Resour. Crop Evol., 43, 13–23.
  14. Narain, A. Prakash, S. (1972). Investigations on the artificial synthesis of amphidiploids of Brassica tournefortii Gouan with the other elementary species of Brassica. 1. Genomic relationships. Genetica, 43, 90–97.
  15. Nei, M., Li, M.H. (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Nat. Acad. Sci., 76, 5269–5273.
  16. Perry, M.C., McIntosh, M.S. (1991). Geographical patterns of variation in the USDA soybean germplasm collections: I. Morphological traits. Crop Sci., 31, 1350–1355.
  17. Pires, J.C., Zhao, J.W., Schranz, M.E., Leon, E.J., Quijada, P.A. (2004). Flowering time divergence and genomic rearrangements in resynthesized Brassica polyploids (Brassicaceae). Biol. J. Linn. Soc. London, 82, 675–688.
  18. Shah, J.A., Mujtaba, M., Khurshid, H. (2018). Estimation of spatial genetic structure in inter-regional populations of Trigonella foenum-graceum L. species through phenotypic variation and seed protein profiling. Genetika, 50(1), 171–185. DOI.org/10.2298/GENSR1801171S
  19. Stephanie, U., Celucia, R.C., Pena., Villa, N.O. (2009). Genetic characterization of Brassica rapa chinensis L., B. rapa parachinensis (L.H. Bailey) Hanelt, and B. oleracea alboglabra (L.H. Bailey) hamlet using simple sequence repeat markers. Phil. J. Sci., 138, 141–152.
  20. Thakur, K.A., Kunwar, S.H., Lal, S., Joghee, N., Yasin, K.J., Dhiraj, S. (2018). SSR marker variations in Brassica species provide insight into the origin and evolution of Brassica amphidiploids. Hereditas, 6, 155. DOI 10.1186/s41065-017-0041-5
  21. Thakur, K.A., Kunwar, S.H., Lal, S., Joghee, N., Yasin, K.J., Dhiraj, S. (2017). Patterns of subspecies genetic diversity among oilseed Brassica rapa as revealed by agro-morphological traits and SSR markers. J. Plant Biochem. Biotechnol., 26, 282–292.
  22. Turi, N.A., Farhatullah, Rabbani, M.A., Shinwari, Z.K. (2012). Genetic diversity in the locally collected brassica species of Pakistan based on microsatellite markers. Pak. J. Bot., 44, 1029–1035.
  23. www. Brassicainfo.com
  24. Xu, J., Qian, X., Wang, X., Li, R. (2010). Construction of an integrated genetic linkage map for the A genome of Brassica napus using SSR markers derived from sequenced BACs in B. rapa. BMC Genomics, 11, 594. DOI 10.1186/1471-2164-11-594

Downloads

Download data is not yet available.

Most read articles by the same author(s)