Essential oil (ЕО) from the leaves of wild-growing Micromeria thymifolia (Scop.) Fritsch (Lamiaceae) was screened for its chemical composition as well as its possible antioxidant and antibacterial properties. According to the gas chromatography – flame ionization detector (GC-FID) and gas chromatography – mass spectrometry (GC-MS) analyses, twenty-six compounds were identified, representing approximately 99.0% of the chromatographic area. The major component present in the essential oil was pulegone (44.8%), other main constituents were piperitone oxide (14.5%), iso-menthone (9.3%) and limonene (8.0%). The oil was tested for the antioxidant activity in 2,2-diphenyl-1-picryl hydrazyl (DPPH) assay and showed a dose-dependent free radical scavenging activity with EC50 value of 6.71 × 104 μg/ml. The antibacterial effect of the essential oil was tested against Gram-negative and Gram-positive bacteria. The essential oil of M. thymifolia showed antioxidant potential and antibacterial activity.


Micromeria thymifolia; essential oil; GC-FID; GC-MS; antioxidant; antibacterial

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Published : 2021-08-31

Marin, M., & Branković, S. (2021). CHEMICAL COMPOSITION AND BIOLOGICAL ACTIVITY OF THE ESSENTIAL OIL OF WILD-GROWING Micromeria thymifolia (Scop.) Fritsch. Acta Scientiarum Polonorum Hortorum Cultus, 20(4), 69-76.

Marija Marin
Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia  Serbia
Snežana Branković 
Faculty of Science, Institute of Biology and Ecology, University of Kragujevac, Radoja Domanovića 12, 34 000 Kragujevac, Serbia  Serbia

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