Improving faba bean seed yield, protein and chlorophyll content by foliar application of humic acid


Humic acid (HA) can be considered as an important alternative to chemical fertilizers in sustainable agriculture. For this purpose, an experiment was conducted for two years (2014–2015) as a factorial in the form of randomized complete block design (RCBD) with three replications in Chalous city. First factor included time of application (T1 – application of HA before planting, T2 – spraying HA at middle of vegetative growth, T3 – spraying HA at early time of reproductive growth). Second factor included the amount of HA as foliar application at four levels (0 (control), 200, 300 and 400 mg l–1 ha–1). The results of the mean comparison show that the highest seed yield was obtained by spraying HA at 200 and 300 mg l–1 ha–1 at the beginning time of reproductive growth and recorded 2821 and 2773 kg ha–1, respectively. The results also show that the highest percentage of protein were recorded by foliar application of HA at 300 mg l–1 ha–1 during T2 treatment. The use of HA at 200 and 400 mg l–1 ha–1 treatments and T2 treatment had the highest protein percentage and protein yield. The results showed that the use of HA at different concentrations had a positive effect on chlorophyll a, b and total chlorophyll, seed yield and protein yield. Also, the time of application of HA had a significant impact on the measured traits. It seems that foliar application at the early period of reproductive growth has improved growth, increased flower fertility, and seed yield by increasing the absorption of nutrients. According to the results, foliar spraying with 300 mg l–1 ha–1 HA at the T3 is the most appropriate treatment for proper faba bean.


foliar application time; humic concentrations; protein yield; photosynthetic pigments; Vicia faba

Abdel-Mawgoud, A.M.R., El-Greadly, N.H.M., Helmy, Y.I., Singer, S.M. (2007). Responses of tomato plants to different rates of humic based fertilizer and NPK fertilization. J. Appl. Sci. Res., 3(2), 169–174.
Albayrak, S., Camas, N. (2005). Effects of different levels and application times of humic acid on root and leaf yield and yield components of forage turnip (Brassica rapa L.). J. Agron., 4, 130–133.
Arnon, D.I. (1967). Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Am. Soc. Plant Physiol., 24, 1–10.
Atiyeh, R.M., Lee, S., Edwards, C.A., Arancon, N.Q., Metzger, J.D. (2002). The influence of humic acids derived from earthworm-processed organic wastes on plant growth. Biores. Technol., 84(1), 7–14.‏
Blandino, M., Badeck., F.W., Giordano., D., Marti., A., Rizza., F., Scarpino. V., Vaccino, P. (2020). Elevated CO2 impact on common wheat (Triticum aestivum L.) yield, wholemeal quality, and sanitary risk. J. Agric. Food Chem., 68, 10574–10585.
Bremner, J.M., Mulvaney, C.S. (1982). Nitrogen – total. In: Methods of soil analysis. Part. 2. Chemical and microbiological properties, Page, A.L., Miller, R.H., Keeney, D.R. (eds.). American Society of Agronomy, Soil Science Society of America, Madison, Wisconsin, 595–624.‏
Bulent-Asik, B., Turan, A., Celik, H., Vahap-Katkat, A. (2009). Effects of humic substances on plant growth and mineral nutrients uptake of wheat (Triticum durum cv. Salihli) under conditions of salinity. Asian J. Crop Sci., 1, 87–95.
Chamani, Khodabandeh, F., Habibi, N., Asgharzadeh, D., DavoudiFard, M. (2012). Effects of salinity stress on yield and yield components of inoculated wheat by plant growth promoting bacteria (Azotobacter chroocccum, Azospirillum lipoferum and Pseudomonase putida) and humic acid. Iran. J. Agron. Plant Breed., 8(1), 37–25.
Cordeiro, F.C., Catarina, C.S., Silveira, V., De Souza, S.R. (2011). Humic acid effect on catalase activity and the generation of reactive oxygen species in corn (Zea mays). Biosci. Biotechnol. Biochem., 75(1), 70–74.
Dawood, M.G., Abdel-Baky, Y.R., El-Awadi, M.E.S., Bakhoum, G.S. (2019). Enhancement quality and quantity of faba bean plants grown under sandy soil conditions by nicotinamide and/or humic acid application. Bull. Natl. Res. Cen., 43(1), 1–8.‏
Delfine, S., Tognetti, R., Desiderio, E., Alvino, A. (2005). Effect of foliar application of N and humic acids on growth and yield of durum wheat. Agron. Sustain. Dev., 25, 183–191.
El-Bassiony, A.M., Fawzy, Z.F., Abd El-Baky, M.M.H., Mahmoud A.R. (2010). The response of snap bean plants to mineral fertilizers and humic acid application. Res. J. Agric. Biol. Sci, INS. Int. Publication., 6(2), 169–175.
El-Desuki, M. (2004). Response of onion plants to humic acid and mineral fertilizer application. Ann. Agric. Sci., 42(4), 1955–1964.
EL-Ghozoli, A.A. (2003). Influence of humic acid on faba bean plants grown in cadmium polluted soil. Ann. Agric. Sci. Moshtohor, 41(4), 1787–1800.
El-Nemr, M.A., El-Desuki, M., El-Bassiony, A.M., Fawzi, Z.F. (2012). Response of growth and yield of cucumber plants (Cucumis sativus L.) to different foliar application of humic acid and bio-stimulators. Aust. J. Basic Appl. Sci., 6(3), 630–637.
Emami, A. (1996). In the description of methods of analysis. Tech. Pub., Soil Res. Inst. Water, 1(982), 91–128.
Ghorbani, S., Khazaei, H., Kafi, M., Banayan Awal, M. (2010). Effect of humic acid and irrigation water on yield and yield components of maize. Agric. Ecol. J., 2(1), 123–131. (In Persian with English abstract)
Giasuddin, A.B., Kanel, S.R., Choi, H. (2007). Adsorption of humic acid onto nanoscale zerovalent iron and its effect on arsenic removal. Environ. Sci. Technol., 41(6), 2022–2027.‏
Karakurt, Y., Unlu, H., Unlu, H., Padem, H. (2009). The influence of foliar and soil fertilization of humic acid on yield and quality of pepper. Acta Agric. Scand. B Soil Plant Sci., 59(3), 233–237.‏
Khan, A., Guramni, A.R., Khan, M.Z., Hussain, F., Akhtar, M.E., Khan, S. (2012). Effect of humic acid on growth, yield, nutrient composition, photosynthetic pigment and total sugar contents of peas (Pisum sativum L.). J. Chem. Soc. Pak., 6, 56–63.
Majnun Husseini, N. (2008). Agriculture and grain production. Jihad-Daneshghahi Pub., Univ. Tehran, 283P. (In Persian)
Meganid, A.S., Al-Zahrani, H.S., EL-Metwally, M.S. (2015). Effect of humic acid application on growth and chlorophyll contents of common bean plants (Phaseolus vulgaris L.) under salinity stress conditions. Int. J. Inn. Res. Sci. Eng. Technol., 4(5), 2651–2660.
Nardi, S., Pizzeghello, D., Muscolo, A., Vianello, A. (2002). Physiological effects of humic substances on higher plants. Soil Biol Biochem., 34(11), 1527–1536.‏
Neri, D., Lodolini, E.M., Savini, G., Sabbatini, P., Bonanomi, G., Zucconi, F. (2002). Foliar application of humic acid on strawberry (cv. Onda), Proceeding 1S on the foliar nutrient, M. Tagliavini et al. (eds.). Acta Hortic., 594, 297–302.
Sabzevari, S., Khazaei, H.R., Kafi, M. (2010). Effects of humic acid on germination of four wheat cultivars (Triticum aestivum L.). Iran. J. Field Crops Res., 8(3), 473–480. (In Persian with English Abstract)
Said-Al Ahl, H.A.H., El Gendy, A.G., Omer, E.A. (2016). Humic acid and in dole acetic acid affect yield and essential oil of dill grown under two different locations in Egypt. J. Pharm. Sci. Res., 8(8), 146–157.
Salimon, J., Salih, N., Yousif, E. (2012). Biolubricant base stocks from chemically modified ricinoleic acid. J. King Saudi Univ., 24, 11–17.
Samavat, S., Malakuti, M. (2005). The important use of organic acid (humic and fulvic) to increase quantity and quality agriculture productions. Water Soil Res. Tech., 463, 1–13.
Saruhan, V., Kusvuran, A., Babat, S. (2011). The effect of different humic acid fertilization on yield and yield components performances of common millet (Panicum miliaceum L.). Sci. Res. Essays, 6(3), 663–669. https://
Schmidt, R.E., Zhang, X. (1998). How humic substances help turfgrass grow. Golf Course Manag., 67(7), 65–68.
Singh, A.K., Bhatt, B.P., Upadhyaya, A., Kumar, S., Sundaram, P.K., Singh, B.K., Chandra, N., Bharati, R.C. (2012). Improvement of faba bean (Vicia faba L.) yield and quality through biotechnological approach: a review. Afr. J. Biotechnol., 11(87), 15264–15271.
Srivastava, H.N. (1995). Mineral nutrition, plant physiology. 7th ed. Pradeep Publications, Jalandhar, pp 137.
Turpin, J.E., Robertson, M.J., Hillcoat, N., Herridge, D.E. (2002). Faba bean (Vicia faba L.) in Australia’s northern grains belt: canopy development biomass and nitrogen accumulation and partitioning. Crop Past. Sci., 53(2), 227–237.
Ulukan, H. (2008). Effect of soil applied humic acid at different sowing times on some yield components in wheat (Triticum spp.) hybrids. Int. J. Bot., 4(2), 164–175.

Published : 2022-04-29

Roudgarnejad, S., Samdeliri, M., Mirkalaei, A., & Moghaddam, M. (2022). Improving faba bean seed yield, protein and chlorophyll content by foliar application of humic acid. Acta Scientiarum Polonorum Hortorum Cultus, 21(2), 115-121.

Samaneh Roudgarnejad 
Department of Agronomy, Chalous Branch, Islamic Azad University, Chalous, Iran  Iran, Islamic Republic of
Morteza Samdeliri
Department of Agronomy, Chalous Branch, Islamic Azad University, Chalous, Iran‏.  Iran, Islamic Republic of
Amirabas Mousavi Mirkalaei 
Department of Agronomy, Chalous Branch, Islamic Azad University, Chalous, Iran  Iran, Islamic Republic of
Mojtaba Nasheai Moghaddam 
Department of Agronomy, Chalous Branch, Islamic Azad University, Chalous, Iran  Iran, Islamic Republic of

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.


Articles are made available under the conditions CC BY 4.0 (until 2020 under the conditions CC BY-NC-ND 4.0).
Submission of the paper implies that it has not been published previously, that it is not under consideration for publication elsewhere.

The author signs a statement of the originality of the work, the contribution of individuals, and source of funding.