IMPROVEMENT OF in vitro PROPAGATION AND ACCLIMATION OF Helichrysum arenarium L. Moench
Anna Figas
University of Science and Technology, Bydgoszcz, PolandMagdalena Tomaszewska-Sowa
University of Science and Technology, Bydgoszcz, PolandAnna Sawilska
University of Science and Technology, Bydgoszcz, PolandAnna J. Keutgen
University of Natural Resources and Life Sciences, Vienna, AustriaAbstract
Explants of apical buds of sandy everlasting Helichrysum arenarium L. Moench sterilized with calcium hypochlorite – Ca(OCl2) were placed onto Murashige- -Skoog (MS) growth medium enriched with 1 mg∙dm-3 KIN (kinetin) in two consecutive passages. To optimize the procedure of in vitro micropropagation, the axillary shoots of Helichrysum arenarium L. Moench were transferred during the third passage onto 9 dif-ferent combinations of MS medium without plant growth regulators (PGRs-free medium), with different concentrations of KIN (1.0, 3.0, 4.0, 5.0 mg∙dm-3) and KIN (1.0, 3.0, 4.0, 5.0 mg∙dm-3) with IAA (indole-3-acetic acid) (0.5 mg dm-3). The highest mean number of shoots (24.7) was observed on MS medium with 5 mg∙dm-3 KIN and 0.5 mg∙dm-3 IAA. The achieved branched shoots were rooted and acclimatized. Rhizogenesis was intensified by the presence of growth hormones: 0.5 mg∙dm-3 IAA or 0.5 mg∙dm-3 IBA (indole-3-butyric acid). At the stage of acclimation of plantlets the application of a water solution of MS salts (25%) for watering the plants increased the efficacy of plant acclimation from 56 to 75%.
Keywords:
sandy everlasting, phytohormones, rooting, in vivo conditions, medicinal plantReferences
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Sawilska, A., Figas, A. (2006). Micropropagation of Helichrysum arenarium (L.) Moench. Inter-national Conference Biotechnology 2006. Česke Budějovice, Czech Republic, 15–16 Feb. 2006, Scientific Pedagogical Publishing, 721–723.
Sawilska, A.K., Mielcarek, S. (2009). The content of flavonoids and polyphenolic acids in inflorescences of Sandy Everlasting (Helichrysum arenarium (L.) Moench) from natural stands and plantations. Herba Pol., 55(3), 118–126.
Sawilska, A.K., Włodarczyk, A. (2014). Sposób otrzymywania sadzonek kocanek piaskowych Helichrysum arenarium (L.) Moench pochodzących z kultur in vitro oraz inicjowania uprawy z siewu niełupek do zmodyfikowanego podłoża. Patent PL 216755; B3. O udzieleniu patentu ogłoszono 30.05.2014 r.
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Stanojević, D., Ćomić, L.J., Stefanović, O., Solujić-Sukdoloak, S. (2010). In vitro synergistic antibacterial activity of Helichrysum arenarium, Inula helenium, Cichorium intybus and some preservatives. Ital. J. Food Sci., 22(2), 210–216.
Tomaszewska-Sowa, M., Figas, A. (2011). Optimization of the processes of sterilization and micropropagation of cup plant (Silphium perfoliatum L.) from apical explants of seedlings in in vitro cultures. Acta Agrobot., 64(4), 3–10.
Albayrak, S., Aksoy, A., Sagdic, O., Hamzaoglu, E. (2010a). Compositions, antioxidant and antimicrobial activities of Helichrysum (Asteraceae) species collected from Turkey. Food Chem., 119, 114–122.
Albayrak, S., Aksoy, A., Sagdic, O., Budak, U. (2010b). Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. Turk. J. Biol., 34, 463–473.
Anbazhagan, M., Dhanavel, D., Kalpana, M., Natarajan, V., Rajendran, R. (2010). In vitro production of Stevia rebaudiana Bertoni. Emir. J. Food Agric., 22, 216–222.
Assim, M., Khawar, K.M., Özcan, S. (2008). In vitro micropropagation from shoot meristems of Turkish cowpea (Vigna unguiculata L.) CV. Akkiz. Bangl. J. Bot., 37(2), 149–154.
Beyl, C.A. (2011). PGRs and their use in micropropagation. In: Plant tissue culture, development, and biotechnology, Trigiano, R.N., Gray, D.J. (eds). CRC Press, Taylor & Francis Group, 33–56. Bryksa-Godzisz, M., Pawełczak, A. (2010). In vitro propagation of the yellow everlasting (Helichrysum arenarium (L.) Moench) from root explants. Propag. Ornam. Plants, 10(1), 14–17.
Chinou, I.B., Roussis, V., Perdetzoglou, D., Loukis, A. (1996). Chemical and biological studies on two Helichrysum species of greek origin. Planta Med., 62, 377–379.
Clasquin, S., Henry, M. (2002). Micropropagation of Helichrysum arenarium L. Moench. Acta Bot. Gallica, 149(2), 189–195.
Czinner, E., Kéry, Á., Hagymási, K., Blázovics, A., Lugasi, A., Szöke, É., Lemberkovics, É. (1999). Biologically active compounds of Helichrysum arenarium (L.) Moench. Eur. J. Drug. Metab. PH., 24(4), 309–313.
Czinner, E., Hagymasi, K., Blazovics, A. (2000). In vitro antioxidant properties of Helichrysum arenarium (L.) Moench. J. Ethnopharm., 73, 437–443.
Eroğlu, H.E., Hamzaoğlu, E., Aksoy, A., Budak, U., Albayrak, S. (2010). Cytogenetic effects of Helichrysum arenarium in human lymphocytes cultures. Turk. J. Biol., 34, 253–259.
Hu, C.Y., Wang, P.J. (1983). Meristem, shoot-tip and bud culture. In: Handbook of plant cell culture, Evans, D.A., Sharp, W.R., Ammirato, P.V., Yamada, Y. (eds). 1, Macmillan, New York, 177–277.
Jitendra, M., Monika, S., Ratan, S.D., Priyanka, G., Priyanka, S., Kiran, D.J. (2012). Micropropagation of an anti diabetic plant – Stevia rebaudiana Bertoni (natural sweetener) in Hadoti Region of South-East Rajasthan, India. Int. J. Biol. Sci., 1(3), 37–42.
Kanwar, J.K., Kumar, S. (2008). In vitro propagation of Gerbera – a review. Hortic Sci. (Prague), 35(1), 35–44.
Lemberkovics, E., Czinner, E., Szentmihalyi, K., Balazs, A., Szoke, E. (2002). Comparative evaluation of Helichrysi flos herbal extracts as dietary sources of plant polyphenols, and macro- and microelements. Food Chem., 78, 119–127.
Murashige, T., Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15, 473–497.
Pawełczak, A., Bryksa-Godzisz, M. (2008). Mikrorozmnażanie kocanek piaskowych (Helichrysum arenarium (L.) moench) z pąków kątowych. Zesz. Probl. Post. Nauk Roln., 527, 247–254.
Pawlaczyk, I., Czerchawski, L., Pilecki, W., Lamer-Zarawska, E., Gancarz, R. (2009). Polyphenolic-polysaccharide compounds from selected medicinal plants of Asteraceae and Rosaceae families: Chemical characterization and blood anticoagulant activity. Carbohyd. Polym., 77, 568–575.
Piękoś-Mirkowa, H., Mirek, Z. (2006). Flora Polski. Rośliny chronione. Multico Oficyna Wydawn., Warszawa, ss. 417.
Radusiene, J., Judzentiene, A. (2008). Volatile composition of Helichrysum arenarium field accessions with differently colored inflorescences. Biologija, 54(2), 116–120.
Sawilska, A., Figas, A. (2006). Micropropagation of Helichrysum arenarium (L.) Moench. Inter-national Conference Biotechnology 2006. Česke Budějovice, Czech Republic, 15–16 Feb. 2006, Scientific Pedagogical Publishing, 721–723.
Sawilska, A.K., Mielcarek, S. (2009). The content of flavonoids and polyphenolic acids in inflorescences of Sandy Everlasting (Helichrysum arenarium (L.) Moench) from natural stands and plantations. Herba Pol., 55(3), 118–126.
Sawilska, A.K., Włodarczyk, A. (2014). Sposób otrzymywania sadzonek kocanek piaskowych Helichrysum arenarium (L.) Moench pochodzących z kultur in vitro oraz inicjowania uprawy z siewu niełupek do zmodyfikowanego podłoża. Patent PL 216755; B3. O udzieleniu patentu ogłoszono 30.05.2014 r.
Sawilska, A. (2015). Biologiczne i ekologiczne uwarunkowania introdukcji kocanek piaskowych Helichrysum arenarium (L.) Moench do uprawy polowej. In: Cenne składniki flory i roślinności na obszarze Pomorza i Kujaw. Monografia, Załuski, T., Krasicka-Korczyńska, E., Ratyńska, H., Sawilska, A.K. (eds). Wyd. Pol. Tow. Botan., Oddz. w Bydgoszczy, 5–20.
Stanojević, D., Ćomić, L.J., Stefanović, O., Solujić-Sukdoloak, S. (2010). In vitro synergistic antibacterial activity of Helichrysum arenarium, Inula helenium, Cichorium intybus and some preservatives. Ital. J. Food Sci., 22(2), 210–216.
Tomaszewska-Sowa, M., Figas, A. (2011). Optimization of the processes of sterilization and micropropagation of cup plant (Silphium perfoliatum L.) from apical explants of seedlings in in vitro cultures. Acta Agrobot., 64(4), 3–10.
Anna Figas
University of Science and Technology, Bydgoszcz, Poland
University of Science and Technology, Bydgoszcz, Poland
Magdalena Tomaszewska-Sowa
University of Science and Technology, Bydgoszcz, Poland
University of Science and Technology, Bydgoszcz, Poland
Anna Sawilska
University of Science and Technology, Bydgoszcz, Poland
University of Science and Technology, Bydgoszcz, Poland
Anna J. Keutgen
University of Natural Resources and Life Sciences, Vienna, Austria
University of Natural Resources and Life Sciences, Vienna, Austria
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